HPTLC Densitogram Profile of Aqueous Distillate (Arka) from Roots of Boerhaavia diffusa

Pooja, .; Lal, V. K.; Verma, Anurag

doi:10.9734/bjpr/2016/19399

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“…The same method was also used in isolation of Boeravinone-D, G and H. Likewise qualitative and quantitative analysis has also been reported through sophisticated and advance chromatographic technique [29][30] . UPLC was used for estimation of eight Boeravinones in methanolic extract of B. diffusa roots using PDA detector 33 .…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Boeravinone-B in dry fruit extract of Boerhaavia diffusa Linn and in its Polyherbal Formulation

Singh

Sharma

Singh

et al. 2017

Journal of Natural Remedies

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Objective: To ascertain the authenticity of Boeravinone-B in Boerhaavia diffusa and its poly-herbal dosage form (capsule) by identification and quantification through precise and robust HPLC method. Materials and Methods: The RP-HPLC analysis was accomplished employing phosphate buffer and acetonitrile as mobile phase at a flow rate of 1.5 ml/min and 270 nm as detection wavelength. Results: The content of Boeravinone B in ingredients (hydro-alcoholic extracts of whole plant) and poly-herbal formulation of B. diffusa was estimated as 0.041% w/w and 0.011% w/w, respectively with good linearity R2>0.9997 and retention time 24.413±0.004 min. Conclusion: The amount of Boeravinone-B present in B.diffusa extracts of raw drug complies with that of reported in literature. Quantification of Boeravinone-B suggested an identification mark for this drug and its different market formulations and may work as quality parameter in standardization. On the basis of percentage of Boeravinone-B the content of B.diffusa in poly-herbal formulation validate the ratio of ingredient in final formulation. This method proved its applicability in quality assessment via quantification of this marker in raw material as well as various phyto-formulations of B. diffusa and widens the acceptability criteria at international level. Identification and Quantification of Journal of natural remedies AbstractIpomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.

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Section: Introductionmentioning

confidence: 99%