HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line

Sun, Suling; Wang, Wei; Wang, Nan; Zhang, Yu; Zhu, Zihang; Li, Xue; Wang, Junhong; Chen, Qihe; Sadiq, Faizan Ahmed; Yang, Hua; Qi, Qianhui; Zhang, Guohua

doi:10.1016/j.jff.2020.104328

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…Other animal-derived peptides from the silkworm pupae (Bombyx mori) proteins have been shown to be a great source of peptides that exert several beneficial effects on human health. Sun et al obtained a silkworm pupae protein hydrolysate by hydrolysis with a neutral proteinase (52 ℃ for 5 h, pH = 7) and observed that the most effective fraction in inhibiting HMG-CoAR activity (53%) contained peptides less than 3 kDa, (89). The peptides HPP, SGQR, QPGP, CQP, and NQR, from this fraction, inhibit HMG-CoAR activity by 84%, 78%, NMAIHPR TNAIPYVR TNAIPYVRYL 56%, 26%, and 17%, respectively.…”

Section: Animal-derived Peptidesmentioning

confidence: 99%

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Food-derived peptides with inhibitory capacity for HMG-CoA reductase activity: a potential nutraceutical for hypercholesterolemia

Sánchez,

López,

España

et al. 2024

Food Science and Human Wellness

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Cardiovascular diseases (CVDs) are the leading global cause of mortality and disease burden. Statins are the most prescribed lipid-lowering drugs to treat hypercholesterolemia and prevent CVDs. The biochemical mechanism of statins consists of competitive inhibition of the 3-hydroxy-3-methylglutaryl-CoA reductase enzyme (HMG-CoAR), the limiting enzyme in cholesterol biosynthesis. Due to statin intolerance in some patient groups, the search for new inhibitors is a field of great interest. This review focusses on the studies reporting the inhibitory effect of protein hydrolysates and biopeptides on the HMG-CoAR enzyme activity. The analysis of the action mechanism and physicochemical characteristics of the HMG-CoAR inhibitory peptides revealed that the molecular weight, amino acid composition, charge, and polarity are key aspects of the interaction with the HMG-CoAR enzyme. In conclusion, this review reveals the potential of using food peptides as new cholesterol-lowering agents and opens a new interesting field of research. However, clinical approaches are mandatory to confirm their therapeutic hypercholesterolemic effect.

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Section: Animal-derived Peptidesmentioning

confidence: 99%

“…The peptides HPP, SGQR, QPGP, CQP, and NQR, from this fraction, inhibit HMG-CoAR activity by 84%, 78%, NMAIHPR TNAIPYVR TNAIPYVRYL 56%, 26%, and 17%, respectively. A molecular docking study revealed that H-bond interactions in the L-domain are responsible for the inhibitory action of HPP and SGQR (89).…”

Section: Animal-derived Peptidesmentioning

confidence: 99%

Food-derived peptides with inhibitory capacity for HMG-CoA reductase activity: a potential nutraceutical for hypercholesterolemia

Sánchez,

López,

España

et al. 2024

Food Science and Human Wellness

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show abstract

“…In HepG2 cells, the HMGCoAR protein displayed substantial upregulation when treated with a PBPH fraction. Sun et al (2021) identified hypocholesterolemic peptides that highly suppress HMGCoAR from silkworm pupae protein hydrolysates that were synthesized by hydrolyzing proteins with neutral proteinase and by purifying various fractions (<3, 3−5, and >5 kDa). Peptides with HMG-CoAR inhibiting potentials were identified employing in silico predictions.…”

Section: Hypocholesterolemic Peptidesmentioning

confidence: 99%

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Patil

Usman

Zhang

et al. 2022

Comp Rev Food Sci Food Safe

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Food‐derived bioactive peptides (BAPs) are recently utilized as functional food raw materials owing to their potential health benefits. Although there is a huge amount of scientific research about BAPs’ identification, purification, characterization, and physiological functions, and subsequently, many BAPs have been marketed, there is a paucity of review on the regulatory requirements, bioavailability, and safety of BAPs. Thus, this review focuses on the toxic peptides that could arise from their primary proteins throughout protein extraction, protein pretreatment, and BAPs’ formulation. Also, the influences of BAPs’ length and administration dosage on safety are summarized. Lastly, the challenges and possibilities in BAPs’ bioavailability and regulatory requirements in different countries were also presented. Results revealed that the human studies of BAPs are essential for approvals as healthy food and to prevent the consumers from misinformation and false promises. The BAPs that escape the gastrointestinal tract epithelium and move to the stomach are considered good peptides and get circulated into the blood using different pathways. In addition, the hydrophobicity, net charge, molecular size, length, amino acids composition/sequences, and structural characteristics of BAPs are critical for bioavailability, and appropriate food‐grade carriers can enhance it. The abovementioned features are also vital to optimize the solubility, water holding capacity, emulsifying ability, and foaming property of BAPs in food products. In the case of safety, the possible allergenic and toxic peptides often exhibit physiological functions and could be produced during the hydrolysis of food proteins. It was also noted that the production of iso‐peptides bonds and undesirable Maillard reaction might occur during protein extraction, sample pretreatments, and peptide synthesis.

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Silkworm pupa protein and its peptides: Preparation, biological activity, applications in foods, and advantages

Wu,

Yang,

Mumby

et al. 2023

Trends in Food Science & Technology

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HPP and SGQR peptides from silkworm pupae protein hydrolysates regulated biosynthesis of cholesterol in HepG2 cell line

Cited by 8 publications

References 42 publications

Food-derived peptides with inhibitory capacity for HMG-CoA reductase activity: a potential nutraceutical for hypercholesterolemia

Food-derived peptides with inhibitory capacity for HMG-CoA reductase activity: a potential nutraceutical for hypercholesterolemia

An updated review on food‐derived bioactive peptides: Focus on the regulatory requirements, safety, and bioavailability

Silkworm pupa protein and its peptides: Preparation, biological activity, applications in foods, and advantages

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