HPLC separation of antibiotics present in formulated and unformulated samples

Joshi, Sujata

doi:10.1016/s0731-7085(01)00706-3

Cited by 95 publications

(55 citation statements)

References 64 publications

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“…However, the major antibiotics used for humans either belong to the same general classes or have the same mode of action as those used for animals [1]. A questionnaire-based survey conducted preliminarily by the authors in this study showed that the most commonly used antibiotics in livestock among major Lebanese dairy farmers are Gentamicin and Streptomycin which belong to the Aminoglycoside group of antibiotics.…”

Section: Introductionmentioning

confidence: 78%

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Zeina¹,

Pamela²,

Fawwak³

2013

FNS

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The rapid growth of dairy sectors in the Middle East, particularly in Lebanon, led to extensive use of antibiotics to enhance the health and productivity of animals. Prolonged usage may lead to antibiotic residues in foods of animal origin; hence, the emergence of antimicrobial resistant microorganisms. Accurate data on the antibiotic usage in livestock treatment, antibiotic residues and antimicrobial resistances in raw milk in Lebanon are lacking. This study aimed to investigate the types and usages of antibiotics in cattle, their residual levels and the potential microbial resistances in raw milk samples. A questionnaire-based survey identified Gentamicin and Streptomycin as the most frequently used antibiotics. Selected raw milk samples from main dairy farms were then analyzed in duplicate by quantitative ELISA for the antibiotics residual levels. The mean residual levels of Gentamicin and Streptomycin were 90 and 80 μg/L, respectively; which are below the allowable maximum residue limit of 200 μg/L as set by the FAO/WHO. Staphylococcus aureus, Listeria monocytogenes, E. coli and total aerobic microorganisms isolated from the milk samples were then tested for resistance against Gentamicin and Streptomycin by the disc agar diffusion method. All the S. aureus, E. coli, and L. monocytogenes isolates showed high resistance to Gentamicin. However, 95% of S. aureus, 60% of E. coli and 58% of L. monocytogenes isolates were resistant to Streptomycin. The obtained results provide evidence that antimicrobial resistant strains of the above pathogens have become remarkably widespread in raw milk. This requires better management for antibiotic usages among livestock farmers to control sources of food contamination and reduce the health risks associated with the development of resistant microbial strains.

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Section: Introductionmentioning

confidence: 78%

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Zeina¹,

Pamela²,

Fawwak³

2013

FNS

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“…Due to the minor structural differences amongst members of the same group, it is difficult to identify and to determine their respective degradation products (Joshi, 2002). Several analytical methods for quantitative determination of fluoroquinolones in pharmaceutical formulations are described in scientific literature, like capillary electrophoresis (Bhowal, Das, 1991;Flurer, 1997;Sun, Chen, 1997), UV spectrophotometry (Fratini, Schapoval, 1996;Chowdary, Rao, 1997), titrimetry (Belal, Al-Majed, Al-Obaid, 1999), and high performance liquid chromatography (HPLC) (United States Pharmacopeia , 2004;British Pharmacopoeia, 1999;Mazuel, 1991), amongst others.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of ciprofloxacin and norfloxacin in pharmaceutical preparations by high performance liquid chromatography

Kassab¹,

Singh²,

Kedor-Hackmam³

et al. 2005

Rev. Bras. Cienc. Farm.

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“…Due to its toxicity vancomycin was relegated to the role of alternate therapy when antibiotics such as methicillin became available 3 . There are several methods to estimate ceftriaxone, including high performance liquid chromatography (HPLC) [4][5][6][7][8] , high performance thin layer chromatography 9 , capillary electrophoresis 10,11 , and spectrophotometry [12][13][14] . In case of vancomycin, it has been assayed by HPLC [15][16][17] , micellar electrokinetic capillary chromatography 18 , and spectrophotometry 19 .…”

Section: Ceftriaxone Is (6r7r) -3 [(Acetyl -Oxy) Methyl] -7 -[[(2z)-mentioning

confidence: 99%

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Tariq¹,

Siddiqui²,

Kumar³

et al. 2010

ScienceAsia

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A reverse phase-liquid chromatographic method with UV detection at 280 nm is described for simultaneous determination of ceftriaxone sodium and vancomycin hydrochloride. Chromatographic separation of the two drugs was achieved on a Betasil C-1 column using a mobile phase consisting of a binary mixture of acetonitrile and triethylamine buffer adjusted to pH 3.5 ± 0.1 with orthophosphoric acid in a ratio of 20:80. The liquid chromatographic method developed offers symmetric peak shape, good resolution, and reasonable retention time for both drugs. Linearity, accuracy, and precision were found to be acceptable over the concentration ranges 125-750 ppm for ceftriaxone and 62.5-375 ppm for vancomycin. The liquid chromatographic method was successfully applied to the quality control of formulated products, plasma, and cerebrospinal fluid samples containing ceftriaxone and vancomycin.

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HPLC separation of antibiotics present in formulated and unformulated samples

Cited by 95 publications

References 64 publications

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Quantification of Antibiotic Residues and Determination of Antimicrobial Resistance Profiles of Microorganisms Isolated from Bovine Milk in Lebanon

Quantitative determination of ciprofloxacin and norfloxacin in pharmaceutical preparations by high performance liquid chromatography

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