HPLC Separation and Quantitative Determination of Ginsenosides from Panax ginseng, Panax quinquefolium and from Ginseng Drug Preparations

Abstract

A new HPLC-method for separation and quantitative determination of ginsenosides in Panax ginseng, Panax quinquefolium and in pharmaceutical drug preparations is elaborated. A reversedphase-system with pBondapak C18 column (3.9 mm I. D . x 30 cm) using acetonitrile-water (30:70) 2 ml/min and acetonitrile-water (18:82) 4 ml/min is suitable for the base-line separation of Rb,, Rbz, Rc, Rd, Rf, R k , respectively Re, Rg, in 30 minutes. The ginsenosides are directly detected at 203 nm (without derivatization) with the LC-55 or LC-75 spectrophotometer (Perkin-Elmer) at 100 % transmission. Detection limit is 300 ng at a signal-to-noise ratio of 10:l.The calibration curve of each ginsenoside has a correlation coefficient very near to 1. Relative standard deviation for quantitative determinations depends upon the amount of ginsenosides and is approximately I % for ginsenoside contents of 1 %. This method is adaptable for routine analysis in quality control laboratories.

Quality Control of Panax Species by Monoclonal Antibody and DNA Analysis

“…The concentrations determined by ELISA agreed well with those determined by HPLC (Kitagawa et al, 1987). ELISA was more sensitive than TLC (Tani et al, 1981) or HPLC (Sticher and Sticher, 1979;Soldati and Sticher, 1980). Moreover, Yamaguchi et al (1988) and Samukawa et al (1995) reported the comparative concentrations of ginsenosides in various commercial ginseng radices analyzed by HPLC.…”

Determination of Polyacetylenes and Ginsenosides in Panax Species Using High Performance Liquid Chromatography

“…The detection limits of these ginsenosides were 0.033-0.056 mg (calculated into mg unit) in this study. The detection limits of these ginsenosides were reported to be 0.3 mg by Soldati et al 18) and 0.09-0.20 mg by Samukawa et al 19) Therefore, our method was excellent with regard to detection ability of ginsenosides.…”

Age‐ and individual‐related specificities in the effects of standardized ginseng extract on learning and memory (experiments on rats)

“…GI 15 represents as active principles 4% of the specific for ginseng triterpene saponins called ginsenosides (Sticher and Soldati, 1979;Soldati and Sticher, 1980;Schulten and Soldati, 198 I).…”