HPLC Separation and Quantitative Determination of Biflavones in Leaves fromGinkgo biloba

Abstract

An HPLC method for separation and quantitative determination of biflavones in crude leaf extracts from GINKGO BILOBA L. is described. A system using a Lichrosorb(R)-Diol column and the ternary elution system: hexane-chloroform-tetrahydrofuran, was suitable for separation of sciadopitysin, ginkgetin, isoginkgetin and bilobetin. The biflavones were detected at 330 nm; their calibration curves were established and their response factors were calculated using acacetin as internal standard. This method was used for the quantitative determination of the biflavones in crude leaf extracts; the seasonal variations of the biflavone content were studied and showed a higher amount in autumn leaves.

Seasonal Variations of the Flavonoid Content fromGinkgo bilobaLeaves

“…All the HPLC traces show five distinct groups of substances: 1) the very polar ones, like phenolic and kynurenic acids (compounds 1-2), 2) the flavonol glycosides (3-8), 3) the acylfiavonol glycosides (9-10), 4) the flavone dimers (11-14), 5) two unidentified peaks (15)(16). amount is near than that of the acylfiavonol glycosides.…”

“…This ancient tree also synthesises dimeric flavonoids derived from amentoflavone (5) the pharmacological properties of which are very interesting (6)(7)(8)(9)(10)(11)(12)(13)(14). In previous papers we studied their separation by HPLC (15) and showed that their seasonal variations presented higher amounts at the end of the vegetative cycle (16).…”

Inhibition of Mast Cell Histamine Release by Flavonoids and Biflavonoids

“…Luteolin was also prepared by hydrolysis of luteolin-7-glycoside, which was extracted from the leaves of Hieracium pilosella L. (45). Sciadopitysin, ginkgetin and bilobetin were purified from a crude mixture extracted from green Ginkgo biloba L. leaves, according to the techniques already developed (46,47). Amentoflavone was prepared from leaves of Viburnum lantana L., using a reported procedure (48).…”

Quantitative determination of bilobetin in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study

“…Bilobetin (Figure 1) is a biflovone isolated from the leaves of Ginkgo biloba (Briançon-Scheid, Lobstein-Guth, & Anton, 1983). The compound exerts a variety of pharmacological activities: (a) it inhibits the production of the inflammatory cytokines, cyclooxygenase and nitric oxide in macrophases (Baek et al, 1999), cAMP-phosphodiesterase in adipose tissue (Saponara & Bosisio, 1998) and human thrombin (Chen et al, 2019); (b) it suppresses lymphocyte proliferation (Lee et al, 1995); (c) it ameliorates hyperlipidemia, lipotoxicity and insulin resistance (Kou et al, 2012); and (d) it stimulates osteoblast differentiation (Lee et al, 2006).…”