HPLC Sample Injection and Column Switching

Harvey, Mack C.; Stearns, Stanley D.

doi:10.1007/978-1-4612-5306-8_7

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Either an air actuated Valco Model CV-6-UHPa-N6O six-port valve or an electrically actuated, Valco Model ACF 4U.2 (0.2 p1) valve was used for injection. With the latter valve, a moving injection technique (13) was employed in order to minimize extracolumn dispersion in the injection valve.…”

Section: Methodsmentioning

confidence: 99%

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Gradient liquid chromatography/mass spectrometry using microbore columns and a moving belt interface

Hayes¹,

Schwartz²,

Vouros³

et al. 1984

Anal. Chem.

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1229X lo4 C cm-2 for T = 0.03 s, 0.06 s, and 0.12 s, respectively. (This gives a diffusion coefficient of 1.26 X cm2 s-l for tert-butyl p-toluate). This indicates that the process responsible for the second electron observed in the coulometry experiments is negligible in the potential step experiment. The discrepancies between the two experiments are probably due to different time scales and initial ester concentrations: 50 mM and 2 h for coulometry vs. 1.05 mM and 0.12 s for the longest potential step experiment.A better indication of the validity of an assumed mechanism is obtained by analyzing numerous current ratio points from each experiment. Using parameter values similar to those found assuming an EC mechanism, ECE DISPl data files were simulated and then analyzed by the EC nonlinear least-squares method. As shown in Figure 3, the resulting "best fit" did not match the simulated data. Even worse matches were obtained for simulated ECE data.Registry No. tert-Butyl p-toluate, 13756-42-8. LITERATURE CITED(1) Schwarz, W. M.: Shah I.

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Section: Methodsmentioning

confidence: 99%

“…The heat is supplied electrically and controlled by a feedback system to maintain a constant level of vaporization. The optimal temperature (12)(13)(14).…”

Section: Determination Of Nonderivatized Peptides By Thermospray Liqumentioning

confidence: 99%

Gradient liquid chromatography/mass spectrometry using microbore columns and a moving belt interface

Hayes¹,

Schwartz²,

Vouros³

et al. 1984

Anal. Chem.

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“…Atualmente, válvulas de até 4 nL podem ser encontradas comercialmente, no entanto, caso se necessite de volumes de injeção menores que aqueles fornecidos por tais válvulas, pode-se ainda empregar diversas técnicas alternativas de injeção descritas na literatura [24][25][26][27] , as quais serão discutidas detalhadamente em outra parte desta série de artigos.…”

Section: Dispersão Devido à Introdução Da Amostraunclassified

Cromatografia líquida capilar: 2. Alargamento das bandas por efeitos extracoluna

Coutinho¹,

Lanças

2011

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ResumoO alargamento da banda é um fator altamente indesejável em todas as técnicas cromatográficas, uma vez que diminui a eficiência da coluna (menor número de pratos, N) e deteriora a resolução (menor Rs). Este efeito é particularmente crítico na cromatografia líquida capilar (c-LC) devido aos pequenos volumes da instrumentação, conexões, colunas, etc., empregados na técnica. Como parte de uma série de artigos a respeito da c-LC, neste trabalho serão abordados os principais fatores que ocasionam o alargamento das bandas cromatográficas em c-LC, assim como a maneira de evitá-lo. Palavras-chaveCromatografia líquida capilar; alargamento de bandas; fatores extra coluna; micro LC; LC capilar. Capillary liquid chromatography: 2. Band broadening due to extracolumn effects AbstractBand broadening is one of the major factors to be avoided in chromatographic techniques, once it decreases the column efficiency (the number of plates, N) and deteriorates the Resolution (Rs). This effect is particularly critical in capillary liquid chromatography due to the very low volumes, instrumentation, connections and columns employed in this technique. As a part in a series of papers dealing with c-LC, this work describes the major factors responsible for band broadening in chromatography, as well as the ways to minimize it.

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Application of a nested loop system for the flow injection analysis of trace aqueous peroxides

Dasgupta¹,

Hwang²

1985

Anal. Chem.

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HPLC Sample Injection and Column Switching

Cited by 4 publications

References 27 publications

Gradient liquid chromatography/mass spectrometry using microbore columns and a moving belt interface

Gradient liquid chromatography/mass spectrometry using microbore columns and a moving belt interface

Cromatografia líquida capilar: 2. Alargamento das bandas por efeitos extracoluna

Application of a nested loop system for the flow injection analysis of trace aqueous peroxides

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