HPLC profiling of Phellinus linteus

Abstract

HPLC chromatograms of MeOH extracts from a fruit body of the wild-grown P. linteus (natural fruit body), from cultivated fungus (cultivated fruit body), and from the cultured mycelia were compared. The extract prepared from the natural fruit bodies revealed a typical HPLC profile referred to as type 1 with a major peak corresponding to meshimakobnol A (1) together with two minor peaks of hypholomine B (3) and inoscavin A (4); the cultivated fruit bodies exhibited a profile referred to as type 2 with major peaks corresponding to 3 and 4 and a minor peak of 1, and the cultured mycelia showed a profile referred to as type 3 without any of these peaks. We also analyzed HPLC chromatograms of commercial products of P. linteus obtained in the markets. Most of the products claimed to be natural fruit bodies exhibited type 1 profiles, except for one product having an intermediate HPLC profile between type 1 and type 2. The products claimed to be cultivated fruit bodies and cultured mycelia revealed type 2 and type 3 profiles, respectively. The present results indicate that the HPLC chromatogram of the methanol extract of P. linteus can be used as a fingerprint to identify whether the product is from natural fruit bodies, cultivated fruit bodies, or cultured mycelia.

Simultaneous Quantification of Antioxidant Compounds in Phellinus igniarius Using Ultra Performance Liquid Chromatography-Photodiode Array Detection-Electrospray Ionization Tandem Mass Spectrometry

“…Moreover, Inoscavin A and Hypholomine B were not well separated using the reported HPLC method [ 14 ], with the running time about 14min. Because the main structure of these two composition were same, their chromatographic behavior were similar, only when the initial ratio of organic solvent was low, can a necessary separation efficiency be gotten.…”

“…High-speed counter-current chromatography (HSCCC) is a support-free liquid-liquid partition chromatography technique that is widely used for the purification of natural products [ 8 , 9 ], including the purification of polyphenols from natural sources [ 10 , 11 ]. The structure of these two polyphenols has been carefully evaluated using nuclear magnetic resonance spectroscopy (NMR), mass spectrometry (MS) [ 12 , 13 ] and ultraviolet spectrometry (UV) [ 14 ]. The data was contained in supporting information and proved being in accordance with the previously researching report [ 12 , 15 ].…”

Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance

“…[69][70][71] Styrylpyrone composition of wild-grown and cultivated P. linteus and the cultured mycelia differed from each other. 72 This may be attributed to environmental factors, such as microbial attack, ultraviolet radiation and oxidative stress. Nevertheless, the cultivated mushroom or cultured mycelia of P. linteus exhibited biological activities comparable to the wild-grown mushroom, and produced an abundant amount of yellow pigments containing mainly styrylpyrones with various biological activities.…”

“…Nevertheless, the cultivated mushroom or cultured mycelia of P. linteus exhibited biological activities comparable to the wild-grown mushroom, and produced an abundant amount of yellow pigments containing mainly styrylpyrones with various biological activities. 28,29,34,[52][53][54][55][56][57]60,64,[72][73][74][75] In addition, the adverse effects of the medicinal mushrooms Phellinus and Inonotus have not been reported until now. Methanolic extracts of I. xeranticus did not show acute toxicity up to 2 g kg À1 in mice, and the LD 50 values of these extracts were above 2 g kg À1 , indicating the safe nature of these mushrooms.…”

A rapid analysis of antioxidants in Sanghuangporus baumii by online extraction-HPLC-ABTS

“…Two peaks, protocatechuic acid (1) and protocatechualdehyde (2), were identied by comparing the retention time and the mass spectrometric data of the chromatographic peaks with that of the reference substances. By verifying the mass spectrometry data of the chromatographic peak with the reported data, [23][24][25][26] 7), and inoscavin A (8). The detailed mass data is provided in Table 2.…”