A rapid analysis of antioxidants in <i>Sanghuangporus baumii</i> by online extraction-HPLC-ABTS

Shen, Qianhui; Huang, Qi; Xie, Jianliang; Wang, Kun; Qian, Zhengming; Li, Deqiang

doi:10.1039/d1ra04300e

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…Owing to the fact that the chromatogram of the second extraction was stable and no residual peak was detected ( Figure 2 (C)), it was shown that the sample could be completely extracted in the first online extraction. The high extraction efficiency could be attributed to the high pressure of the OLME system, which allows better access of the mobile phase inside the matrix and ensures better extraction [ 17 , 18 ]. The method is green and efficient, with minimal sample consumption, and can be easily applied to the analysis of natural products or other solid materials, especially for those samples that are difficult to obtain or more valuable.…”

Section: Resultsmentioning

confidence: 99%

Online Microextraction Coupled with HPLC‐ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta

Yang

Qian

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive compounds from PB is inadequate. In the current research, an online microextraction (OLME) coupled with a high-performance liquid chromatography coupled with the 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay (HPLC-ABTS) system for rapid analysis of antioxidants from PB was proposed. The PB sample (0.17 mg) was online extracted by mobile phase (acetonitrile and 0.2% acetic acid); a Poroshell 120 SB-Aq column was used for separation; then, an online ABTS assay system was used for screening the antioxidants. Finally, ten components were found in PB, and among them, eight components possessed antioxidant activities. Furthermore, five components (gallic acid, neochlorogenic acid, caffeic acid, chlorogenic acid, and an unknown compound) were proved as major antioxidants when compared with rutin as an antioxidant marker. The results showed that the developed OLME-HPLC-ABTS system was a simple, rapid, green, and efficient instrument for the screening of antioxidants from PB, which provides a powerful tool for the discovery of natural antioxidants in Chinese medicines.

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Section: Resultsmentioning

confidence: 99%

Online Microextraction Coupled with HPLC‐ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta

Yang

Qian

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…However, ethanol extracts also contain other components, such as sesquiterpenoids and carbonyl compounds’ derivatives, suggesting that compounds in the Sanghuang species might not be exclusive to a few categories [ 32 , 101 ]. Some changes of the solvent might occur in some research papers, such as replacing ethanol with 70% methanol or absolute alcohol [ 9 , 25 , 28 ].…”

Section: Extraction Methodsmentioning

confidence: 99%

“…Sanghuang was also mentioned in the Compendium of Materia Medica in the Ming Dynasty as a traditional medicinal mushroom taken orally to eliminate the body’s toxins [ 2 , 21 ]. Entering modern times, pharmaceutical research has demonstrated multiple functions of Sanghuangporus spp., including the ability to improve blood circulation, scavenge free radicals, demonstrate anti-inflammatory effects, anti-carcinogenesis, antioxidative abilities, immunomodulatory effects, antidiabetic activities, antimicrobial abilities, and antiaging effects [ 2 , 4 , 9 , 14 , 20 , 21 , 22 , 24 , 25 , 26 , 27 , 28 , 29 ]. Some research has also provided evidence of antigout, sleep-improving, neuroprotective, and hepatoprotective activities [ 3 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…Mycelia, conversely, demonstrate higher levels of polysaccharides, proteins, and other bioactive content than the fruiting bodies [ 15 , 50 ]. The compounds in these two parts provide Sanghuang with many valuable functions, including antitumor activities and free-radical scavenging abilities [ 6 , 9 , 13 , 16 , 20 , 25 , 26 , 28 , 35 , 37 , 51 , 52 , 53 , 54 ]. Polysaccharides are reported to be the major medical component and possess anti-inflammatory, antioxidative, antitumor, diabetes-ameliorating, sleep-improving, and neuroprotective effects, often demonstrated through pathway regulation, free-radical scavenging, and metabolic regulation [ 3 , 10 , 15 , 32 , 34 , 50 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

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Research Progress of Bioactive Components in Sanghuangporus spp.

Lu,

Su,

Zhou

et al. 2024

Molecules

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The species in Sanghuangporus are a group of edible mushrooms with a long history of oral use in East Asia as a health-improvement method. They should be classified under the genus Sanghuangporus rather than mistakenly in Phellinus or Inonotus. The major components in this genus consist of polysaccharides, polyphenols, triterpenoids, and flavonoids, all of which exist in the fruiting bodies and mycelia. For extraction, studies have shown methods using hot water, ethanol, DES solvent, and alkaline, followed by purification methods including traditional anion column, Sevag solution, macroporous resin, and magnetic polymers. Proven by modern medical technology, these components possess promising anti-inflammatory, antioxidative, antitumor, and immunoregulation effects; additionally, they have health-improving effects including pulmonary protection, hypoglycemic properties, sleep improvement, gout mitigation, antiaging, neuroprotection, and muscle-strengthening abilities. Several toxicity studies have revealed their safety and recommend a dose of 1 g/kg for mice. As a newly emerged concept, functional food can provide not only life-sustaining nutrients but also some health-improving effects. In conclusion, we substantiate Sanghuang as a functional food by comprehensively presenting information on extraction and purification methods, component medical and structural properties, and nontoxicity, hoping to benefit the development of Sanghuang species as a group of functional food.

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“…Rutin is a polyphenolic flavonoid with anti-inflammatory and antioxidant properties and is of significant medical importance [ 24 ]. The quantitative analysis of active ingredients in Sanghuangporus is crucial for evaluating its quality, and identifying antioxidants from it can enhance the accuracy of product quality assessment [ 25 ]. Therefore, searching for potential biomarkers in flavonoids may become a crucial criterion for evaluating the efficacy of Sanghuangporus therapy.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Flavonoids in Fruiting Bodies of Sanghuangporus Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

et al. 2023

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A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of Sanghuangporus was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Separation was achieved using a ZORBAX Eclipse Plus C18 column (1.8 μm, 3.0 mm × 100 mm) with a 15 min gradient of a mobile phase consisting of 0.01% aqueous formic acid and 2 mm/L ammonium formate (mobile phase A), and 0.01% formic acid and 2 mm/L ammonium formate in methanol (mobile phase B). A mass spectrometry analysis was performed using the multiple reaction monitoring (MRM) mode with an electrospray ion source. This method enabled the simultaneous detection of 10 flavonoids (sakuranetin, quercitrin, myricitrin, kaempferol, luteolin, rutin, hyperoside, kaempferol-3-O-rutinoside, catechin, and catechin gallate) in the fruiting bodies of Sanghuangporus. Additionally, we applied this method to analyze the flavonoid content in fruiting bodies of various Sanghuangporus species. The results revealed substantial variations in flavonoid content, up to a 100-fold difference, among different species, with myricitrin, hyperoside, and rutin identified as the most abundant flavonoids. This protocol serves as a valuable tool for quantifying flavonoid compounds in different Sanghuangporus species or under diverse cultivation conditions, particularly for identifying species with high levels of specific flavonoid compounds.

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A rapid analysis of antioxidants in Sanghuangporus baumii by online extraction-HPLC-ABTS

Abstract: Rapid discovery of antioxidants from natural product by online OLE-HPLC-ABTS.

Cited by 5 publications

References 17 publications

Online Microextraction Coupled with HPLC‐ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta

Online Microextraction Coupled with HPLC‐ABTS for Rapid Analysis of Antioxidants from the Root of Polygonum bistorta

Research Progress of Bioactive Components in Sanghuangporus spp.

Quantitative Analysis of Flavonoids in Fruiting Bodies of Sanghuangporus Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

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