HPLC-MS analysis of Schisandra lignans and their metabolites in Caco-2 cell monolayer and rat everted gut sac models and in rat plasma

Yang, Jiaming; Ip, Siu‐Po; Yeung, Hok-keung J.; Che, Chun‐Tao

doi:10.1016/j.apsb.2011.04.007

Cited by 17 publications

(11 citation statements)

References 21 publications

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“…The chemical ingredients of the Schisandra extract were characterized by a validated HPLC-DAD method (Yang et al, 2011c). The major lignans in the extract were well separated (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study has identified a number of Schisandra lignans, including schisandrin, gomisin A and deoxyschisandrin, in rat plasma after oral administration of S. chinensis extract (Yang et al, 2011c). A previous report also claimed that schisandrin can cross blood-brain barrier and distribute in different regions of brain (Niu et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

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Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Yang

Xian

et al. 2012

Phytomedicine

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Visceral hypersensitivity is an important characteristic feature of functional gastrointestinal disorders, such as irritable bowel syndrome (IBS). This study evaluated the effect of Schisandra chinensis on visceral hyperalgesia induced by neonatal maternal separation (NMS) in an IBS rat model. The visceromotor responses to colorectal balloon distension (CRD) were measured by abdominal withdrawal reflex (AWR) and electromyographic activities (EMG). NMS control rats (receiving vehicle) underwent aggravated visceral pain in response to CRD as compared to normal rats, evidenced by the reduced pain threshold, enhanced AWR scores and EMG responses. Treatment with a 70% ethanol extract of S. chinensis (0.3 g/kg and 1.5 g/kg per day) for seven days resulted in an increase in the pain threshold (NMS control: 19.1 ± 1.0 mmHg vs low-dose: 24.8 ± 1.3 mmHg and high-dose: 25.2 ± 1.8 mmHg, p<0.01), and abolished the elevated AWR and EMG responses to CRD in NMS rats (AUC values of EMG response curve were: 1952 ± 202 in NMS control group vs 1074 ± 90 in low-dose group and 1145 ± 92 in high-dose group, p<0.001), indicating that S. chinensis could reverse the visceral hypersensitivity induced by early-life stress event. The result of ELSA measurement shows that the elevated serotonin (5-HT) level in the distal colon of NMS rats returned to normal level after treatment with S. chinensis. Moreover, the increase in pain threshold in rats treated with S. chinensis was associated with a decline of the mRNA level of 5-HT3 receptor in the distal colon. All available results demonstrate that S. chinensis can reverse visceral hypersensitivity induced by neonatal-maternal separation, and the effect may be mediated through colonic 5-HT pathway in the rat.

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“…The chemical ingredients of the Schisandra extract were characterized by a validated HPLC-DAD method (Yang et al, 2011c). The major lignans in the extract were well separated (Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Yang

Xian

et al. 2012

Phytomedicine

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“…The results of UHPLC-MS/MS analyses are summarized in Table S2. The presence of dibenzocyclooctadiene lignans (schisandrin, gomisin G, schisantherin A and B, deoxyschisandrin and schisandrin C), dibenzylbutane lignans (hernicine B) and aryltetralin lignans (wulignan A1 and A2, epiwulignan A1, enshicine, epienshicine and dimethylwulignan A1) was confirmed (Table S2) (Taguchi and Ikeya 1975;Ikeya et al 1979aIkeya et al , b, 1980Ikeya et al , 1982aIkeya et al , b, 1986Ikeya et al , 1988Ikeya et al , 1990Jia-Sen et al 1988;Liu et al 1988;He et al 1997;Deng et al 2008;Wang et al 2011;Yang et al 2011). Moreover, kadsuric acid and schisanhenric acid, from the triterpendoids group, were confirmed by UHPLC-MS/MS analysis (Table S2) (Jia-Sen et al 1980;Zou et al 2015).…”

Section: Uhplc-ms/ms Qualitative Analysesmentioning

confidence: 85%

Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds

Jafernik

Szopa

Barnaś

et al. 2020

Plant Cell Tiss Organ Cult

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We initiated and optimized in vitro culture conditions of the endemic Chinese plant species—Schisandra henryi C. B. Clarke. Different types of in vitro solid cultures (microshoot and callus), cultivation periods (10, 20, and 30 days), and selected concentrations of BA, IBA, GA3 (0 to 3 mg/l) in the Murashige and Skoog (MS) medium were tested. The presence of dibenzocyclooctadiene lignans (schisandrin, gomisin G, schisantherin A and B, deoxyschisandrin and schisandrin C), dibenzylbutane lignans (hernicine B), aryltetralin lignans (wulignan A1 and A2, epiwulignan A1, enshicine, epienshicine and dimethylwulignan A1), and triterpenoids: kadsuric acid and schisanhenric acid was confirmed by UHPLC–MS/MS analysis. Using HPLC–DAD, the qualitative and quantitative profiles of dibenzocyclooctadiene lignans, phenolic acids and flavonoids in methanolic extracts from biomass were estimated. The maximum total amounts of these groups of metabolites were 873.71, 840.89 and 421.98 mg/100 g DW, respectively. The main compounds were: schisantherin B (max. 622.59 mg/100 g DW), schisantherin A (max. 143.74 mg/100 g DW), neochlorogenic acid (max. 472.82 mg/100 g DW), caftaric acid (max. 370.81 mg/100 g DW), trifolin (max. 138.56 mg/100 g DW) and quercitrin (max. 122.54 mg/100 g DW). The highest total amounts of secondary metabolites estimated in the extracts from in vitro cultures were, respectively, 13.0, 7.0, and 1.4 times higher than in the leaf extracts analyzed for comparison. This is the first report on the biosynthetic potential of cells from Schisandra henryi in vitro cultures.

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“…Schisandrin A inhibits dengue viral replication [ 22 ]. These lignans are known to be absorbed in rat everted gut sac, human Caco-2 cell monolayer, and in vivo rat models [ 23 ]. These reports support the fact that the major lignans in Omija beverages fermented with sugar, a commonly consumed product, would be absorbed by the body and exert various biological activities.…”

Section: Resultsmentioning

confidence: 99%

Dibenzocyclooctadiene Lignans in Plant Parts and Fermented Beverages of Schisandra chinensis

Park

Koo

Bae

et al. 2021

Plants

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The fruit of Schisandra chinensis, Omija, is a well-known traditional medicine used as an anti-tussive and anti-diarrhea agent, with various biological activities derived from the dibenzocyclooctadiene-type lignans. A high-pressure liquid chromatography-diode array detector (HPLC-DAD) method was used to determine seven lignans (schisandrol A and B, tigloylgomisin H, angeloylgomisin H, schisandrin A, B, and C) in the different plant parts and beverages of the fruit of S. chinensis grown in Korea. The contents of these lignans in the plant parts descended in the following order: seeds, flowers, leaves, pulp, and stems. The total lignan content in Omija beverages fermented with white sugar for 12 months increased by 2.6-fold. Omija was fermented for 12 months with white sugar, brown sugar, and oligosaccharide/white sugar (1:1, w/w). The total lignan content in Omija fermented with oligosaccharide/white sugar was approximately 1.2- and 1.7-fold higher than those fermented with white sugar and brown sugar, respectively. A drink prepared by immersion of the fruit in alcohol had a higher total lignan content than these fermented beverages. This is the first report documenting the quantitative changes in dibenzocyclooctadiene-type lignans over a fermentation period and the effects of the fermentable sugars on this eco-friendly fermentation process.

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HPLC-MS analysis of Schisandra lignans and their metabolites in Caco-2 cell monolayer and rat everted gut sac models and in rat plasma

Cited by 17 publications

References 21 publications

Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Schisandra chinensis reverses visceral hypersensitivity in a neonatal–maternal separated rat model

Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds

Dibenzocyclooctadiene Lignans in Plant Parts and Fermented Beverages of Schisandra chinensis

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