HPLC Method Validation for the Estimation of Lignocaine HCl, Ketoprofen and Hydrocortisone: Greenness Analysis Using AGREE Score

Abstract

In the current study, the reversed-phased high-pressure liquid chromatography (RP-HPLC) method was proposed for the estimation of lignocaine hydrochloride (LIG), hydrocortisone (HYD) and Ketoprofen (KET) according to International Conference for Harmonization (ICH) Q2 R1 guidelines, in a gel formulation. The chromatographic evaluation was executed using Shimadzu RP-HPLC, equipped with a C8 column and detected using UV at 254 nm wavelength, using acetonitrile and buffer (50:50) as a mobile phase and diluent, at flow rate 1 mL/min and n injection volume of 20 μL. The retention time for LIG, HYD, and KET were 1.54, 2.57, and 5.78 min, correspondingly. The resultant values of analytical recovery demonstrate accuracy and precision of the method and was found specific in identification of the drugs from dosage form and marketed products. The limit of detection (LOD) for LIG, HYD, and KET were calculated to be 0.563, 0.611, and 0.669 ppm, while the limit of quantification (LOQ) was estimated almost at 1.690, 1.833, and 0.223 ppm, respectively. The AGREE software was utilized to evaluate the greenness score of the proposed method, and it was found greener in score (0.76). This study concluded that the proposed method was simple, accurate, precise, robust, economical, reproducible, and suitable for the estimation of drugs in transdermal gels.

Validation of a Novel RP-HPLC Technique for Simultaneous Estimation of Lignocaine Hydrochloride and Tibezonium Iodide: Greenness Estimation Using AGREE Penalties

“…The 'a' represents the distance of the peak of the drug from the frontal half-side to the mid-point of the peak and is evaluated at 5% altitude. Similarly, 'b' refers to the length from the center of the peak to the trailing side of the peak (estimated at 5% peak height) [35].…”

An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant

“…Previous literature reports have described the quantication of both drugs for various purposes. Methods for quantifying LD were developed in conjunction with preservatives 18 and other drugs, serving as antiseptics, 19 antiparasitic, 20 antifungal, 20 anti-inammatory, 21 analgesic, 22 mucolytic, 23 antiviral 24,25 and corticosteroids. 21 Additionally, other validated methods exist for determining the drug in polymeric matrices, [26][27][28] plasma, 29,30 and in new topical dosage forms.…”

“…Methods for quantifying LD were developed in conjunction with preservatives 18 and other drugs, serving as antiseptics, 19 antiparasitic, 20 antifungal, 20 anti-inammatory, 21 analgesic, 22 mucolytic, 23 antiviral 24,25 and corticosteroids. 21 Additionally, other validated methods exist for determining the drug in polymeric matrices, [26][27][28] plasma, 29,30 and in new topical dosage forms. 25,[31][32][33] Methods for the quan-tication of TZ, on the other hand, were described for analysing the drug alone, 34,35 concurrently with other drugs like muscle relaxants, 36 analgesics, [37][38][39] and anti-inammatories, [40][41][42][43][44][45][46][47][48] and from pharmaceutical formulations.…”

Quantitative Determination of Some Non-Steroidal Anti-Inflammatory Drugsand Their Acid Dissociation Constants by Direct Potentiometry

“…Various methods such as titrimetry, spectrophotometry, and spectrofluorimetry have been reported for the determination of these drugs [12][13][14][15]. Analysis based on capillary zone electrophoresis, HPLC, and GC-MS methods [16][17][18][19][20] are also available. However, recently the use of electrochemical techniques has been proposed, such as voltammetry, where boron-doped diamond electrodes have been used to study and evaluate the different ibuprofen redox processes [21,22].…”