HPLC Method for the determination of Paeoniflorin inPaeonia Lactiflare Pall and its preparations

Zhou, Mi; Hong, Cai; Z, Huang; Sun, Yongqi

doi:10.1002/(sici)1099-0801(199801/02)12:1<43::aid-bmc730>3.0.co;2-0

Cited by 15 publications

(5 citation statements)

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“…In the present study, therefore, we investigated the time course of paeoniflorin in rat hippocampus after intravenous administration of Paeoniae Radix extract. Though a few HPLC-UV methods were reported to determine paeoniflorin in crude drugs containing Paeoniae Radix [28,29] and in animals' plasma after oral administration [30,31], little work of paeoniflorin on cerebral nucleus has been carried out due to the lack of methodologies that meet the requirements of sensitivity and specificity for paeoniflorin determination in hippocampus. In the present study, we successfully developed a rapid and simple HPLC method to determine paeoniflorin in rat hippocampus.…”

Section: Introductionmentioning

confidence: 99%

Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract

Xing

Ding

et al. 2004

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract

Xing

Ding

et al. 2004

Journal of Chromatography B

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“…Obtaining useful chemical information from samples containing mixtures of CHMs consisting of hundreds of thousands of components has long been a challenging task to chemists and analysts 4, 5. To date, many analytical methods have been developed for analysis of the constituents in RPR, including high‐performance liquid chromatography (HPLC),6, 7 capillary electrophoresis (CE),8 high‐speed counter‐current chromatography (HSCCC),9 and nuclear magnetic resonance (NMR) 10, 11. These methods provided very limited information on the structural elucidation of monoterpene glycosides and galloyl glucosides.…”

mentioning

confidence: 99%

High‐speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high‐performance liquid chromatography coupled with diode‐array detection and time‐of‐flight mass spectrometry

Liu

et al. 2008

Rapid Comm Mass Spectrometry

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A fast high-performance liquid chromatography (HPLC) method coupled with diode-array detection (DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) has been developed for rapid separation and sensitive identification of major constituents in Radix Paeoniae Rubra (RPR). The total analysis time on a short column packed with 1.8-microm porous particles was about 20 min without a loss in resolution, six times faster than the performance of a conventional column analysis (115 min). The MS fragmentation behavior and structural characterization of major compounds in RPR were investigated here for the first time. The targets were rapidly screened from RPR matrix using a narrow mass window of 0.01 Da to restructure extracted ion chromatograms. Accurate mass measurements (less than 5 ppm error) for both the deprotonated molecule and characteristic fragment ions represent reliable identification criteria for these compounds in complex matrices with similar if not even better performance compared with tandem mass spectrometry. A total of 26 components were screened and identified in RPR including 11 monoterpene glycosides, 11 galloyl glucoses and 4 other phenolic compounds. From the point of time savings, resolving power, accurate mass measurement capability and full spectral sensitivity, the established fast HPLC/DAD/TOFMS method turns out to be a highly useful technique to identify constituents in complex herbal medicines.

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“…Thus investigation of the constituents of herbs may be important in the search for new bioactive compounds. Previously, the characterization of monoterpene glycosides has been reported using high‐performance liquid chromatography,9, 10 capillary electrophoresis (CE),11 high‐speed counter‐current chromatography (HSCCC),12 and nuclear magnetic resonance (NMR) 13, 14. With the development of soft ionization techniques, electrospray ionization tandem mass spectrometry (ESI‐MS/MS) has become a powerful technique for the identification and characterization of constituents of TCM, owing to its high sensitivity, rapid analysis time and low levels of sample consumption.…”

mentioning

confidence: 99%

Structural analysis of monoterpene glycosides extracted from Paeonia lactiflora Pall. using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

Dong

Liu

Song

et al. 2007

Rapid Comm Mass Spectrometry

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Paeoniflorin standard was first investigated by electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) using a sustained off-resonance irradiation (SORI) collision-induced dissociation (CID) method at high mass resolution. The experimental results demonstrated that the unambiguous elemental composition of product ions can be obtained at high mass resolution. Comparing MS/MS spectra and the experimental methods of hydrogen and deuterium exchange, the logical fragmentation pathways of paeoniflorin have been proposed. Then, the extracts of the traditional Chinese medicine Paeonia lactiflora Pall. were analyzed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS). By comparison with the ESI-FTICR-MS/MS data of paeoniflorin, the isomers paeoniflorin and albiflorin in Paeonia lactiflora Pall. have been identified using HPLC/MS with CID in an ion trap and in-source CID. Furthermore, using the characteristic fragmentation pathways, the retention times (t(R)) in HPLC and MS/MS spectra, the structures of three other kinds of monoterpene glycoside compounds have been identified on-line without time-consuming isolation. Thus an HPLC/ESI-MS method for the analysis of constituents in Paeonia lactiflora Pall. has been established.

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HPLC Method for the determination of Paeoniflorin inPaeonia Lactiflare Pall and its preparations

Cited by 15 publications

References 0 publications

Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract

Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract

High‐speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high‐performance liquid chromatography coupled with diode‐array detection and time‐of‐flight mass spectrometry

Structural analysis of monoterpene glycosides extracted from Paeonia lactiflora Pall. using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry

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