HPLC investigation of water soluble ternary polyacrylic acid‐Cu<sup>2+</sup>‐protein complexes

Taşdelen, Betül; Bayülken, Seval

doi:10.1002/pat.535

Cited by 6 publications

(5 citation statements)

References 6 publications

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“…2c), the disappearance of the band around 600 8 nm indicates that the doping occurs at the imine segment of the emeraldine chain (Wang et al, 9 2014). The observed bathochromic (red) shift of π-polaron to > 750 nm illustrates that PANI 10 backbone was well doped with -COOH/acetate functional groups in PVIA (Taşdelen, 2017). 11…”

Section: Uv-visible Spectroscopy 30mentioning

confidence: 92%

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

Al-Sagur

Komathi

Karakaş

et al. 2018

Biosensors and Bioelectronics

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The facile preparation of highly sensitive electrochemical bioprobe based on lutetium phthalocyanine incorporated silica nanoparticles (SiO(LuPc)) grafted with Poly(vinyl alcohol-vinyl acetate) itaconic acid (PANI(PVIA)) doped polyaniline conducting nanobeads (SiO(LuPc)PANI(PVIA)-CNB) is reported. The preparation of CNB involves two stages (i) pristine synthesis of LuPc incorporated SiO and PANI(PVIA); (ii) covalent grafting of PANI(PVIA) onto the surface of SiO(LuPc). The morphology and other physico-chemical characteristics of CNB were investigated. The scanning electron microscopy images show that the average particle size of SiO(LuPc)PANI(PVIA)-CNB was between 180-220nm. The amperometric measurements showed that the fabricated SiO(LuPc)PANI(PVIA)-CNB/GOx biosensor exhibited wide linear range (1-16mM) detection of glucose with a low detection limit of 0.1mM. SiO(LuPc)PANI(PVIA)-CNB/GOx biosensor exhibited high sensitivity (38.53µAmMcm) towards the detection of glucose under optimized conditions. Besides, the real (juice and serum) sample analysis based on a standard addition method and direct detection method showed high precision for measuring glucose at SiO(LuPc)PANI(PVIA)-CNB/GOx biosensor. The SiO(LuPc)PANI(PVIA)-CNB/GOx biosensor stored under refrigerated condition over a period of 45 days retains ~ 96.4% glucose response current.

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Section: Uv-visible Spectroscopy 30mentioning

confidence: 92%

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

Al-Sagur

Komathi

Karakaş

et al. 2018

Biosensors and Bioelectronics

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“…Soluble polymer-protein complexes were studied by a wide range of methods, which are well known in colloid and polymer chemistry. 13,[22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] Interaction of polyelectrolytes with proteins in aqueous solutions is found to be sensitive to pH and ionic strength, as well as be dependent on isoelectric points of proteins. 14,37,40 Polymer-protein complexes form as a result of the electrostatic interaction of polyion chains with the oppositely charged groups of the protein molecules.…”

Section: Introductionmentioning

confidence: 99%

“…The complex formation between PEs and proteins has been as subjects of numerous researches. Soluble polymer–protein complexes were studied by a wide range of methods, which are well known in colloid and polymer chemistry 13, 22–39. Interaction of polyelectrolytes with proteins in aqueous solutions is found to be sensitive to pH and ionic strength, as well as be dependent on isoelectric points of proteins 14, 37, 40.…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatography study of water‐soluble complexes and covalent conjugates of polyacrylic acid with bovine serum albumin

Akkiliç

Mustafaeva

Mustafaev

2007

J of Applied Polymer Sci

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Polycomplex formation of bovine serum albumin (BSA) and polyacrylic acid (PAA) was studied by pH titration, fluorescence, and HPLC methods in water solutions. It was shown that the complex formation and the solubility (phase state) of the polycomplexes depended on pH of the solutions and ratio of the components. The stability of PAA-BSA complexes was negligibly weak when pH ¼ 6.0-7.0 [pH > pI (isoelectric pH)]. Stable water-soluble polycomplexes formed at pH ¼ pI (pH 5.0) in a wide range of n BSA /n PAA ratios (0.05-50) and coexisted with free protein molecules at the higher ratios of components. Existence of water-soluble and insoluble PAA-BSA complexes has been observed at pH 4.5. The soluble to insoluble state transition of polycomplexes has been confirmed and binding mode of polyelectrolytes to proteins was assumed dependent on the ratio of components as a result of formation of soluble polycomplexes, complex coacervation, or amorphous precipitates. Soluble complexes were formed as fully homogenized mixtures. Fraction composition of the mixtures and insoluble complexes depended on the protein/polymer ratio and over the critical protein/polymer ratio, the soluble polycomplexes coexisted with free BSA molecules. On the other hand, the comparision of covalent conjugate formation and complexation of PAA with BSA has been revealed.

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“…Many methods were used for the determination of association constant including potentiometric titration 3, HPLC 4, 5, X‐ray absorption near‐edge structure spectroscopy 6 and UV–Vis spectrophotometric titration and 1 H NMR 7.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the interaction of cefadroxil with different metal ions using CE

et al. 2009

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The present study describes the application of CE to investigate the interaction between a cephalosporin, cefadroxil and different metal ions. This study aims at quantifying the interaction of cefadroxil with Zn(2+), Al(3+), Fe(3+), Cu(2+) and Co(2+) ions using the effective electrophoretic mobility as a parameter for calculation of the reaction association constant (K). For this purpose, the electrophoretic mobilities of cefadroxil at different metal ion concentrations in citrate buffer pH 3.6 were determined. A mathematical model to calculate the association constant between cefadroxil and metal ions was used. The results showed that a strong change in the cefadroxil electrophoretic mobility was observed by increasing the metal ion concentrations. The results revealed that by increasing the concentration of metal ions in the running buffer the degree of complexation increases too. On the basis of the results, the strength of the interaction of cefadroxil with the investigated metal ions follows the order Zn(2+)>Cu(2+)>Co(2+)>Fe(3+)>Al(3+). The association constant of the investigated reactions ranged from 451.57 to 1546.52 L/mmol. The results indicate that it is possible to characterize the interaction between cefadroxil and metal ions quantitatively using CE.

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HPLC investigation of water soluble ternary polyacrylic acid‐Cu²⁺‐protein complexes

Cited by 6 publications

References 6 publications

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

High performance liquid chromatography study of water‐soluble complexes and covalent conjugates of polyacrylic acid with bovine serum albumin

Characterization of the interaction of cefadroxil with different metal ions using CE

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HPLC investigation of water soluble ternary polyacrylic acid‐Cu2+‐protein complexes

Cited by 6 publications

References 6 publications

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

A glucose biosensor based on novel Lutetium bis-phthalocyanine incorporated silica-polyaniline conducting nanobeads

High performance liquid chromatography study of water‐soluble complexes and covalent conjugates of polyacrylic acid with bovine serum albumin

Characterization of the interaction of cefadroxil with different metal ions using CE

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HPLC investigation of water soluble ternary polyacrylic acid‐Cu²⁺‐protein complexes