Characterization of the interaction of cefadroxil with different metal ions using CE

Abstract

The present study describes the application of CE to investigate the interaction between a cephalosporin, cefadroxil and different metal ions. This study aims at quantifying the interaction of cefadroxil with Zn(2+), Al(3+), Fe(3+), Cu(2+) and Co(2+) ions using the effective electrophoretic mobility as a parameter for calculation of the reaction association constant (K). For this purpose, the electrophoretic mobilities of cefadroxil at different metal ion concentrations in citrate buffer pH 3.6 were determined. A mathematical model to calculate the association constant between cefadroxil and metal ions was used. The results showed that a strong change in the cefadroxil electrophoretic mobility was observed by increasing the metal ion concentrations. The results revealed that by increasing the concentration of metal ions in the running buffer the degree of complexation increases too. On the basis of the results, the strength of the interaction of cefadroxil with the investigated metal ions follows the order Zn(2+)>Cu(2+)>Co(2+)>Fe(3+)>Al(3+). The association constant of the investigated reactions ranged from 451.57 to 1546.52 L/mmol. The results indicate that it is possible to characterize the interaction between cefadroxil and metal ions quantitatively using CE.

Precision in affinity capillary electrophoresis for drug–protein binding studies

“…Recently, CE once more proved advantageous as a rapid and simple screening method [48], which provides quantitative results of the interactions of drugs with metal ions. For high throughput screenings of several drugs with one protein, the present work suggests a reduced experimental design in which single experiment could suffice, later repeating the experiments for substances with interesting binding properties.…”

Effect of Different Metal Ions on the Biological Properties of Cefadroxil

“…We could not investigate the effect of Cu 2+ ions on the flux of cefadroxil due to the instability of its solution with cefadroxil at 37 °C. In a previous study [3] we determined the association constant of the interaction of cefadroxil with zinc and with aluminium ions. Our results indicated that the reaction strength between cefadroxil and metals used follows the order: Zn 2+ > Al 3+ with association constants of 1546.52 and 451.57 L/mmol respectively.…”

“…The details of preparation and characterisation of cefadroxil-metal complexes were mentioned in our previously published paper [3]. The Caco-2 human colon carcinoma cell line was routinely cultured with minimum essential medium supplemented with 10% fetal bovine serum, 1% nonessential amino acid solution, and gentamicin (45 µg/mL) [9].…”

“…It was reported that cefadroxil can form complexes with different metal ions [1,2]. The interaction between cefadroxil and different metal ions was characterized by capillary electrophoresis [3]. …”

Analytical Comparison of Cefadroxil Determination by Square Wave Adsorptive Stripping Voltammetric and Spectrophotometric Methods

“…Several analytical methods such as high performance liquid chromatography (HPLC) [7,11,12], liquid chromatography mass spectrometry (LC-MS) [13], electro chromatography [14,15], capillary zone electrophoresis [16][17][18][19], chemiluminescence [20][21][22], spectrophotometry [23][24][25] and electroanalytical techniques [26,27] were reported in the literature for the quantification and detection of the CFDL. Among them, spectrophotometry and electrochemical methods are preferable because ease of use, ecofriendly, and costeffective techniques for routine analysis of CFDL in complex matrices.…”