HPLC-fluorescence detection and adsorption of bisphenol A, 17β-estradiol, and 17α-ethynyl estradiol on powdered activated carbon

Yoon, Yeomin; Westerhoff, Paul; Snyder, Shane A.; Esparza, Mario

doi:10.1016/s0043-1354(03)00239-2

Cited by 289 publications

(149 citation statements)

References 37 publications

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“…Similar wavelengths were recorded by Van Emmerik et al, (2003) and Yoon et al, (2003). The HP 1050 HPLC -System consisted of a 79852A Quaternary Pump equipped with a 79855A 21-vial position autosampler, a G1303A vacuum helium degasser, computer and monitor, chemstation, 7125 injector valve and a 1046A fluorescence detector, using methanol and water of a 55:45 ratio as mobile phase.…”

Section: Analysis Of Edcsmentioning

confidence: 96%

Mechanistic evaluation of the sorption properties of endocrine disrupting chemicals in sewage sludge biomass

Ifelebuegu

Theophilus

Bateman

2010

Int. J. Environ. Sci. Technol.

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This study investigated the sorption behaviour of two endocrine disrupting chemicals; 17β-estradiol (E2) and 17β-ethinylestradiol and their thermodynamic properties in an activated sludge biomass. The partition coefficient values measured for E2 and EE2 at varying temperatures range from 245 -604 L/kg ( log K d 2.39 -2.78) and 267 -631 L/kg (Log K d 2.43 -2.80), respectively. The K d values were inversely related to temperature. The average percentages of E2 and EE2 adsorbed to the solid phase at 4.3 % dry solid were 87.2 % and 92.5 %, respectively. Sorption of E2 and EE2 to the activated sludge biomass was found to be spontaneous and entropy retarded with ∆G values in the range of -13 to -16 KJ/mol and ∆S value of -105.2J/mol/K and 96.7 J/mol/k for E2 and EE2, respectively. The enthalpy changes for E2 and EE2 were -45.7KJ/mol and -43.4KJ/mol respectively, demonstrating that the sorption process is exothermic. The values of the enthalpy changes also show that the mechanism of sorption is physisorption with some element of chemisorption.

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Section: Analysis Of Edcsmentioning

confidence: 96%

Mechanistic evaluation of the sorption properties of endocrine disrupting chemicals in sewage sludge biomass

Ifelebuegu

Theophilus

Bateman

2010

Int. J. Environ. Sci. Technol.

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“…Concentrations of BPA were measured using a modification of the method of Yoon et al (2003), with HPLC and fluorescence detection. Water samples (35 L) were injected onto a Synergi-Hydro RP-C18 column (2 × 50 mm; Phenomenex) and eluted isocratically with 40% acetonitrile/phosphate buffer (15 mM monobasic, pH 3.3) at a flow rate of 0.20 mL/min at 40 • C. The BPA was detected using excitation and emission wavelengths of 275 and 300 nm, respectively.…”

Section: Chemical Measurementsmentioning

confidence: 99%

Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

et al. 2010

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a b s t r a c tVarious assays with adult fish have been developed to identify potential endocrine-disrupting chemicals (EDCs) which may cause toxicity via alterations in the hypothalamic-pituitary-gonadal (HPG) axis. These assays can be sensitive and highly diagnostic for key mechanisms such as agonism of the estrogen and androgen receptors (ERs, ARs) and inhibition of steroid synthesis. However, most of the tests do not unambiguously identify AR antagonists. The purpose of this work was to explore the utility of a mixture test design with the fathead minnow (Pimephales promelas) for detecting different classes of EDCs including AR antagonists. Adults of both sexes were exposed via the water to EDCs with diverse mechanisms of action in the absence or presence of 17␤-trenbolone (TB), a potent AR agonist which masculinizes female fathead minnows. Similar to previous studies with the model AR antagonists flutamide and vinclozolin, exposure of females to the AR antagonist cyproterone acetate in the presence of TB decreased expression of an easily-observed masculinization response, nuptial tubercle formation. Mixture studies with TB and the model ER agonists, 17␣-ethinylestradiol and bisphenol A, also showed inhibition of tubercle formation in the females, but unlike the AR antagonists, the estrogens markedly induced synthesis of vitellogenin (VTG: egg yolk protein), particularly in males. The ER agonists also offset TB-induced depressions in plasma VTG concentrations in female fish. Additional mixture experiments were conducted with TB and triclocarban, an anti-microbial reported to enhance AR-mediated responses, or ammonia, a "negative control" with no known direct effects on HPG function. Neither chemical affected VTG status in males or females in the absence or presence of TB; however, both slightly enhanced TB-induced tubercle formation in females. Based on studies described herein and elsewhere with the fathead minnow, a TB co-exposure assay appears to be an effective approach for clearly identifying AR antagonists as well as potential EDCs with other relevant mechanisms of action.Published by Elsevier B.V.

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“…The fluorescence detector was set at 275 nm (excitation wavelength) and 315 mn (emission wavelength) according to a previous report [14].…”

Section: Detection Conditionsmentioning

confidence: 99%

“…However, some catechol estrogens were not sensitively detected by the ECD (e. g., 2-hydroxyestriol). Fluorescence detection [14] and post-labeling detection using fluorescence [15] have been reported for sensitive detection of estrogens. Therefore, a fluorescence detector (FL) was also introduced before the ECD, and could improve the sensitivity for catechol estrogens and also confirm each catechol estrogen peak.…”

Section: Introductionmentioning

confidence: 99%

HPLC for stress‐free screening of potential prostate cancer marker catechol estrogens in urine using a diamond‐electrode electrochemical and a fluorescence detector

Katayama

Takamatsu

Kaneko

et al. 2007

J of Separation Science

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Short CommunicationHPLC for stress-free screening of potential prostate cancer marker catechol estrogens in urine using a diamond-electrode electrochemical and a fluorescence detector Improvement of the sensitivity and specificity of a simultaneous stress-free screening method for catechol estrogens as a potential prostate cancer marker in urine has been accomplished by HPLC with a diamond-electrode electrochemical detector and a fluorescence detector. Since taking urine samples generates less stress (or pain) than the drawing of blood, the method can readily be applied to almost any patient, and will also assist in improving the sensitivity and specificity of the prostatic specific antigen test. Catechol estrogens (2-hydroxyestrone, 4-hydroxyestrone, 2-methoxyestrone, 2-hydroxyestradiol, 4-hydroxyestradiol, 2-methoxyestradiol, and 2-hydroxyestriol) and estrogens (estrone, estradiol, estriol) were separated on an Inertsil ODS-II column with acetonitrile -potassium dihydrogen phosphate (pH 3.0). The diamond-electrode electrochemical detector used had the great advantage of being a maintenance-free system, and could sequentially analyze hundreds of samples. Fluorescence detection improved the sensitivity 10 -500 times (e. g., the LOD of 2-hydroxyestriol was improved 250 times) compared to previous electrochemical detection reports, and dual detection improved peak identification in the urine samples. The proposed method was applied to the simultaneous determination of catechol estrogens in spiked urine in a preliminary study on estrogens and PSA values in biopsy and prostate cancer patients.

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HPLC-fluorescence detection and adsorption of bisphenol A, 17β-estradiol, and 17α-ethynyl estradiol on powdered activated carbon

Cited by 289 publications

References 37 publications

Mechanistic evaluation of the sorption properties of endocrine disrupting chemicals in sewage sludge biomass

Mechanistic evaluation of the sorption properties of endocrine disrupting chemicals in sewage sludge biomass

Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

HPLC for stress‐free screening of potential prostate cancer marker catechol estrogens in urine using a diamond‐electrode electrochemical and a fluorescence detector

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