HPLC Fingerprint of Bioactive Compounds and Antioxidant Activities of Viscum album from Different Host Trees

Abstract

Viscum album L. is a medicinal plants used for many years as a remedy in the traditional medicine and in complementary cancer therapies. We investigated the influence of some host trees: Acer campestre (VAA), Fraxinus excelsior (VAF), Populus nigra (VAP), Malus domestica (VAM), and Robinia pseudoacacia (VAR), on the chemical composition and antioxidant activity of leaves and stems from V. album, as well the influence of the solvent (water and ethanol) used for extraction on biological activity. HPLC with photodiode array detector analysis of bioactive compounds from leaves and stems of mistletoes (V. album) hosted by five differents trees was performed. Antioxidant activities, determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), Oxygen Radical Absorbance Capacity (ORAC), (Trolox-equivalent antioxidant capacity (TEAC) methods were compared with the quantification of total phenolics using the Folin -Ciocalteu reagent. V. album hosted by Fraxinus excelsior (VAF), recorded the highest level of total phenolic acids (108.64 µg/g dry matter) while the mistletoe hosted by Malus domestica (VAM) had the lowest level of total polyphenols (39.37 µg/g dry matter). In general, higher antioxidant activity was detected in the alcoholic than in the aqueous extracts. The values obtained for total phenolics in both, aqueous and ethanol extracts, decreased in the order: VAR > VAF > VAP > VAA > VAM. The mistletoe stem extracts contained lower levels of phenolics, as compared to leaves, in both solvents. Our results suggest that mistletoe extracts possess significant antioxidant activities that may be due to their bioactive compounds content, suggesting that this plant may be an important source of natural products with chemopreventive and chemotherapeutic activities.

Phytochemical and Antioxidant Activity of Camellia Mistletoe (Korthalsella Japonica) Extracts

“…Plant flavonoids have antioxidant activity in vitro and also act as antioxidants in vivo [15]. Recently, Vicas et al [16]reported that the TFC of the ethanol extracts of mistletoe V. album harvested from six different hosts were 0.05-2.19 mg RE/100 g, which is 6.8-324 folds lower than that of the ethanol extract (14.9 mg RE/100 g) in our study (Table 1). This implies that differences of secondary metabolites in mistletoes are influenced by the kind of host plants and the type of solvents used for extraction.…”

Antioxidant Activity of European Mistletoe (Viscum album)

“…Also, like in mistletoe hosted by P. Table 1. Quantitative HPLC analysis of bioactive aglicones of phenolics (µg/g dry matter*) from leaves and stems of V. album harvested from different hosts, on July 2009 (Vicas et al,2011a).…”

“…4. HPLC chromatograms, used to fingerprint and evaluate quantitatively phenolic acids from leaves and stems of V.album harvested from Fraxinus excelsior (VAF) (Vicas et al, 2011a). 3 -protocatechuic acid 5 -chlorogenic acid 6 -para-OH benzoic acid 7 -cafeic acid 8 -syringic acid 9 -salicylic acid 10 -para-coumaric acid 11-ferulic acid 12 -sinapic acid www.intechopen.com…”

“…Where, A 0 is absorbance of the blank, and A S is absorbance of the samples at 515 nm The comparative antioxidant activity of V.album leaves hosted by different host trees, evaluated by the DPPH method shown significantly differences (p < 0.001) regarding to DPPH scavenging effect (%) of aqueous and ethanol extracts in leaves and stems (Vicas et al, 2011a Other research paper (Oluwaseun and Ganiyu, 2008) investigated the antioxidant properties of methanol extracts of V. album isolated from cocoa and cashew trees in the South Western part of Nigeria. The scavenging ability of each methanol extract against DPPH followed a dose-dependent pattern (0-10 mg/ml).…”

Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu

“…Other classes of secondary metabolites from Viscum album are flavonoids, phenylpropanoids, triterpenes, phytosterols, cyclic peptides and cyclitols [6]. Being a parasitic plant, the phenolic content and the antioxidant capacity of mistletoe have been found to vary depending upon the host trees and the time of harvest [9,10]. New compounds have been also reported in mistletoe growing on different hosts [11].…”