HPLC-FID with Superheated Water as the Eluent: Improved Methods and Instrumentation

High-Temperature Separations on a Polymer-Coated Fibrous Stationary Phase in Microcolumn Liquid Chromatography

“…Although the entire separation efficiencies in these chromatograms could not be comparable with that of modern commercially available silica-based stationary phases for high-performance separations, a satisfactory separation ability of the polymer-coated fiber-packed column was obtained without any organic modifier in the mobile phase. Since several advantages of using pure water as the mobile phase have been recently focused, [41][42][43][44][45] the results in Fig. 5 clearly suggest a future possibility of the polymer-coated fibrous stationary phase in micro-LC with pure water as the mobile phase, along with that in microscale sample preparation techniques on the basis of a temperature-programmed operation.…”

Superheated water: the ultimate green solvent for separation science

“…Of greater interest is that water is also compatible with the flame ionisation detection as unlike conventional HPLC mobile phases there is no organic modifier to give a background signal [11,17,18 ]. It can therefore be used for the detection of analytes lacking chromophores, such as aliphatic alcohols, amino-acids and carbohydrates, as the first true universal detector in HPLC (Figure 2).…”

Temperature as a Variable in Pharmaceutical Applications

“…Flame ionization detection (FID) is of particular interest because potentially it offers a sensitive and universal detector. A number of different interfaces have been used, including heated capillaries, which have been examined by Miller and Hawthorne [62], Ingelse et al [63], and others [64,65], who separated a range of analytes including alcohols, amino acids, and phenols. An alternative method employing a cold nebulization of the eluent has been introduced by Bone et al [66].…”