HPLC evaluation of diclofenac in transdermal therapeutic preparations

Klimeš, Jiřı́; Sochor, J; Doležal, Pavel; Körner, J.

doi:10.1016/s0378-5173(01)00594-4

Cited by 24 publications

(14 citation statements)

References 14 publications

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“…Also HPLC methods are available in literature for separate determination of metoprolol tartrate or phenol red [2,[8][9][10][11]. Therefore, the aim of this study was to determine segmental permeability and absorption characteristics of diclofenac using a newly developed and validated reversed-phase liquid chromatographic method for the simultaneous determination of diclofenac, metoprolol tartrate and phenol red in intestinal perfusion samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

Kaynak¹,

Buyutuncel²,

Cagler³

et al. 2015

Trop. J. Pharm Res

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Section: Introductionmentioning

confidence: 99%

Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

Kaynak¹,

Buyutuncel²,

Cagler³

et al. 2015

Trop. J. Pharm Res

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“…However, most of them use high performance liquid chromatography with ultraviolet detection (HPLC-UV) (Arcelloni et al, 2001;Bhattacharya et al, 2013). Therefore, this work methodology was developed based on conditions of analysis described on USP 35 (2013) monograph and on the methodology described by Klimes et al (2001).…”

Section: Resultsmentioning

confidence: 99%

“…The methodology of diclofenac sodium analysis was developed based on conditions described on USP 35 (2013) monograph and also on the methodology described by Klimes et al (2001).…”

Section: Determination Of Diclofenac Sodium In the Samplesmentioning

confidence: 99%

Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion

Andréo

Rufino

Ubaldo

et al. 2016

Braz. J. Pharm. Sci.

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Aiming to alter and/or improve permeation of active compounds in the skin, many strategies have been developed, including biophysical methods. One of the physical absorption techniques, currently known as Cryo Laser Phoresis (CLP), consists of an apparatus that emits radiation on polar or nonpolar molecules of the active substance, resulting in faster penetration when in comparison to the standard topical application. The goal of this work was to evaluate the efficacy of a method that proposes to increase cutaneous permeation of diclofenac sodium by using CLP technique. The influence on permeation was evaluated ex vivo, using Franz cell and human skin obtained from cosmetic surgery. The results were evaluated using statistical methods and data exploratory analysis: clusters, k-means and Principal Component Analysis. The results showed a larger increase in the concentration of diclofenac sodium in the dermis with the use of laser. In all samples (with or without laser application) it was observed that skin surface showed an amount of diclofenac sodium and that there was no active passage to the receptor liquid, suggesting that diclofenac sodium was not absorbed. These results indicate that CLP, when used under the conditions described in this study, is able to increase diclofenac sodium penetration and its retention into deeper layers. Uniterms:Cryo Laser Phoresis/dematologic use. Cutaneous permeation. Diclofenac sodium/cutaneous permeation. Franz cell.No sentido de alterar e/ou melhorar a penetração de substâncias na pele, diversas estratégias têm sido desenvolvidas, variando desde a aplicação de novos veículos e ativos encapsulados, até equipamentos que atuam por métodos biofísicos. Uma das técnicas de absorção física, atualmente conhecida como Crio Laser Forese (CLF), consiste em um aparato que emite radiação sobre moléculas polares ou apolares da substância ativa, tornando sua penetração mais rápida, se comparada à administração tópica comum. O objetivo deste trabalho foi avaliar a eficácia de um método que propõe aumentar a permeação cutânea do diclofenaco de sódio incorporado a um gel, por meio do uso da CLF. A influência sobre a permeação foi avaliada ex vivo, utilizando célula de Franz e pele humana obtida de cirurgia plástica. Os resultados foram balizados mediante aplicação de métodos estatísticos e análise exploratória de dados: clusters, k-means e Análise por Componentes Principais. Os resultados demonstraram aumento na concentração do diclofenaco de sódio na derme com o uso do laser. Em todas as amostras (com ou sem aplicação de laser), observou-se, uma quantidade de diclofenaco de sódio na superfície da pele e que não houve passagem de ativo para o líquido do receptor, sugerindo que o diclofenaco de sódio não foi absorvido. Estes resultados indicam que CLF usada sob as condições descritas neste estudo é capaz de aumentar a penetração do diclofenaco de sódio e sua retenção em camadas mais profundas da pele Unitermos: Crio Laser Forese/uso em dermatologia. Permeação cutânea. Diclofenaco de sódio/perme...

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“…However, we found very poor base line separations of ibuprofen and indomethacin from diclofenac under the experimental conditions used in the present study. Use of another reported internal standard, namely, N-phenylanthranilic acid [27], provided a clear base line separation from diclofenac and 4 -hydroxydiclofenac ( Fig. 1).…”

Section: Choice Of Internal Standardmentioning

confidence: 96%

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

Kaphalia

Kumar

et al. 2006

Journal of Chromatography B

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HPLC evaluation of diclofenac in transdermal therapeutic preparations

Cited by 24 publications

References 14 publications

Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

Performance evaluation of Cryo Laser Phoresis technique as biophysical method to promote diclofenac sodium cutaneous perfusion

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

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