Determination of Regional Intestinal Permeability of Diclofenac and Metoprolol Using a Newly-Developed and Validated High Performance Liquid Chromatographic Method

Abstract

0.319 ± 0.184, 0.639 ± 0.241 and 0.84 3± 0.517 x 10 -4 cm/sec, respectively, for metoprolol tartrate while the corresponding permeability values were 1.585 ± 0.729, 1.154 ± 0.433 and 1.775 ± 1.576 x 10 -4

A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red

“…Also, the paper is presented in the chromatogram obtained by analysis of 100% standard solution under optimized conditions in Figure 1. In the literature, there are few HPLC methods in which PR [19] or both [21,22] as markers for SPIP studies. The optimized method appears to be more successful when evaluated in terms of chromatographic separation of previous MPT and PR analytes [21,22].…”

“…In the literature, there are few HPLC methods in which PR [19] or both [21,22] as markers for SPIP studies. The optimized method appears to be more successful when evaluated in terms of chromatographic separation of previous MPT and PR analytes [21,22]. Since both PR and MPT are highly polar, the organic components of the mobile phase, methanol and C18 in the stationary phase, and phosphate buffer were preferred.…”

Comparison of intestinal permeability of nebivolol hydrochloride loaded solid lipid nanoparticles with commercial nebivolol tablet

“…Animals were allocated one of the four groups given in Table 2. In situ single pass intestinal perfusion test procedure used in this study was a modification of the previously published reports [21][22][23][24][25]. Under anaesthetizia (60 mg/kg pentobarbital, intraperitoneal), the rats in a supine position were placed on an operation table at 37°C.…”

“…Phenol red was used as a zero permeability marker in all groups for correction of permeability coefficients of tested drugs for water transport [24]. Metoprolol tartrate was not used in group 2 and group 3 because NBV Peff value in these groups (group 2 and group 3) was compared with the NBV Peff values in groups 1 and 4.…”

“…In order to prepare SLNs by homogenization technique, compritol, lecithin and poloxamer were selected. For this aim, Single-Pass Intestinal Perfusion (SPIP) method was used for in-situ permeation studies [21][22][23][24][25].…”