Highly sensitive determination of diclofenac based on resin beads and a novel polyclonal antibody by using flow injection chemiluminescence competitive immunoassay

Shi, Jiyong; Xu, Mengmeng; Tang, Qinghui; Zhao, Kang; Deng, Anping; Li, Jianguo

doi:10.1016/j.saa.2017.09.068

Cited by 15 publications

(9 citation statements)

References 32 publications

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“…The CAF LOD of 180 ± 110 ng/L is comparable to indirect CAF ELISAs: Depending on the label and readout, LODs between 83 ng/L and 233 ng/L had been obtained . With the DCF SAFIA a detection limit of 4 ± 3 ng/L was reached, which is also comparable to the ELISA (7.8 ng/L) and ten times more sensitive than an up-conversion ULISA (50 ng/L) and a bead-based chemiluminescence assay (50 ng/L) . The ILA SAFIA shows an LOD of 310 ± 70 ng/L, which is a factor of 3 less sensitive than the ILA ELISA (LOD: 90 ng/L) .…”

Section: Resultsmentioning

confidence: 72%

Wash-Free Multiplexed Mix-and-Read Suspension Array Fluorescence Immunoassay for Anthropogenic Markers in Wastewater

et al. 2019

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Pharmaceuticals, certain food ingredients, and mammalian endogenous metabolic products in wastewater are mostly of human origin. They are anthropogenic markers. Proper knowledge of their levels in wastewater helps to track sources of pollutants in natural waters and allows for calculation of removal efficiencies in wastewater treatment plants. Here, we describe the development and application of an indirect competitive, multiplexing suspension array fluorescence immunoassay (SAFIA) for the detection of carbamazepine (CBZ), diclofenac (DCF), caffeine (CAF), and isolithocholic acid (ILA) in wastewater, covering those classes of anthropogenic markers. The assay consists of haptens covalently conjugated to fluorescence-encoded polystyrene core/silica shell microparticles to create a site for competitive binding of the antibodies (Abs). Bound Abs are then stained with fluorophore-labeled Abs. Encoding and signaling fluorescence of the particles are determined by an automated flow cytometer. For compatibility of the immunoassay with the 96-well microtiter plate format, a stop reagent, containing formaldehyde, is used. This enables a wash-free procedure while decreasing time-to-result. Detection limits of 140 ± 40 ng/L for CBZ, 180 ± 110 ng/L for CAF, 4 ± 3 ng/L for DCF, and 310 ± 70 ng/L for ILA are achieved, which meet the sensitivity criteria of wastewater analysis. We demonstrate the applicability of SAFIA to real wastewater samples from three different wastewater treatment plants, finding the results in good agreement with LC-MS/MS. Moreover, the accuracy in general exceeded that from classical ELISAs. We therefore propose SAFIA as a quick and reliable approach for wastewater analysis meeting the requirements for process analytical technology.

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Section: Resultsmentioning

confidence: 72%

Wash-Free Multiplexed Mix-and-Read Suspension Array Fluorescence Immunoassay for Anthropogenic Markers in Wastewater

et al. 2019

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“…Its aberrant expression has been associated with poor prognosis of prostate cancer, nonsmall cell lung cancer, and colorectal cancer, leading to the suggestion of its use as a novel tumour marker [45][46][47]. SNHG5 (small nucleolar RNA host gene 5) has been strongly implicated in cancer-related processes, such as cell differentiation, cell proliferation, and metastasis [48][49][50]. The strong evidence of the association of all five lncRNAs with cancer and/or OC supports the conclusion that this study identified potential biomarkers for predicting the prognosis of OC patients, which should also help future research into the pathogenesis of OC.…”

Section: Discussionmentioning

confidence: 99%

A novel risk score system for assessment of ovarian cancer based on co-expression network analysis and expression level of five lncRNAs

Zhao

Fan

2019

BMC Med Genet

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Background: Ovarian cancer (OC) is the most deadly gynaecological cancer, contributing significantly to female cancer-related deaths worldwide. Improving the outlook for OC patients depends on the identification of more reliable prognostic biomarkers for early diagnosis and survival prediction. The various roles of long non-coding RNAs (lncRNAs) in OC have attracted increasing attention. This study aimed to identify a lncRNA-based signature for survival prediction in OC patients. Methods: RNA expression data and clinical information from a large number of OC patients were downloaded from a public database. These data were regarded as a training set to construct a weighed gene co-expression network analysis (WGCNA) network, mine stable modules, and screen differentially expressed lncRNAs. The prognostic lncRNAs were screened using univariate Cox regression analysis and the optimal prognosis lncRNA combination was screened using a Cox-PH model. The finalised lncRNA combination was used to construct the risk score system, which was validated and assessed for effectiveness using other independent datasets. Further functional pathway enrichment was performed using gene set enrichment analysis (GSEA). Results: A co-expression network was constructed and four stable modules with OC-related biological functions were obtained. A total of 19 lncRNAs significantly related to prognosis of ovarian cancer were obtained using univariate Cox regression analysis, and the 5 prognostic signature lncRNAs GAS5, HCP5, PART1, SNHG11, and SNHG5 were used to establish a risk assessment system. The reliability of the prognostic scoring system was further confirmed using validation sets, which indicated that the risk assessment system could be used as an independent prognostic factor. Pathway enrichment analysis revealed that the network modules related to the above five prognostic genes were significantly associated with cell local adhesion, cancer signaling pathways, JAK-STAT signalling, and endogenous cell receptor interaction. Conclusions: The risk score system established in this study could provide a novel reliable method to identify individuals at high risk of OC. In addition, the five prognostic lncRNAs identified here are promising potential prognostic biomarkers that could help to elucidate the pathogenesis of OC.

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“…Faster immunoassays developed for DCF comprise an upconversion-linked immunosorbent assay (ULISA) [26], a fluorescence polarization immunoassay (FPIA) [27], and a suspension array fluorescence immunoassay (SAFIA) [28]. Steps forward to mobile and automated sensing of diclofenac have been made with several immunosensors for the detection of DCF reported in recent years [29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac

et al. 2021

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Increasing contamination of environmental waters with pharmaceuticals represents an emerging threat for the drinking water quality and safety. In this regard, fast and reliable analytical methods are required to allow quick countermeasures in case of contamination. Here, we report the development of a magnetic bead-based immunoassay (MBBA) for the fast and cost-effective determination of the analgesic diclofenac (DCF) in water samples, based on diclofenac-coupled magnetic beads and a robust monoclonal anti-DCF antibody. A novel synthetic strategy for preparation of the beads resulted in an assay that enabled for the determination of diclofenac with a significantly lower limit of detection (400 ng/L) than the respective enzyme-linked immunosorbent assay (ELISA). With shorter incubation times and only one manual washing step required, the assay demands for remarkably shorter time to result (< 45 min) and less equipment than ELISA. Evaluation of assay precision and accuracy with a series of spiked water samples yielded results with low to moderate intra- and inter-assay variations and in good agreement with LC–MS/MS reference analysis. The assay principle can be transferred to other, e.g., microfluidic, formats, as well as applied to other analytes and may replace ELISA as the standard immunochemical method. Graphical abstract

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Highly sensitive determination of diclofenac based on resin beads and a novel polyclonal antibody by using flow injection chemiluminescence competitive immunoassay

Cited by 15 publications

References 32 publications

Wash-Free Multiplexed Mix-and-Read Suspension Array Fluorescence Immunoassay for Anthropogenic Markers in Wastewater

Wash-Free Multiplexed Mix-and-Read Suspension Array Fluorescence Immunoassay for Anthropogenic Markers in Wastewater

A novel risk score system for assessment of ovarian cancer based on co-expression network analysis and expression level of five lncRNAs

A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac

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