HPLC Determination of Free and Bound Amino Acids in Bryonia alba

Karpyuk, U. V.; Кисличенко, В. С.; Gur’eva, I. G.

doi:10.1007/s10600-015-1298-4

Cited by 6 publications

(5 citation statements)

References 2 publications

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“…This library largely consisted of plants with ethnopharmacological relevance that have previously been identified as a rich source of cyclotides such as Viola odorata , Viola tricolor , Psychotria solitudinum , Palicourea tomentosa and Carapichea ipecacuanha ( Koehbach et al, 2013 ; Fahradpour et al, 2017 ; de Veer et al, 2019 ). Furthermore, the plant extract library included species that are rich in other cyclotide-like or knottin peptides such as Bryonia alba , Beta vulgaris , Sambucus nigra as well as Momordica charantia ( He et al, 2013 ; Karpyuk et al, 2015 ; Álvarez et al, 2018 ; Retzl et al, 2020 ). Using solvent and solid phase extraction yielded the plant extracts enriched in cysteine-rich peptides.…”

Section: Resultsmentioning

confidence: 99%

“…To this end, we established a library of plant extracts largely consisting of species with ethnopharmacological relevance that have previously been identified as a rich source of cyclotides or cyclotide-like knottin peptides. This encompasses ten plant species: Viola odorata , Viola tricolor , Psychotria solitudinum , Palicourea tomentosa , Carapichea ipecacuanha, Bryonia alba , Beta vulgaris , Sambucus nigra as well as Momordica charantia ( He et al, 2013 ; Koehbach et al, 2013 ; Karpyuk et al, 2015 ; Fahradpour et al, 2017 ; Álvarez et al, 2018 ; de Veer et al, 2019 ; Retzl et al, 2020 ). Using solvent and solid-phase extraction yielded the plant extracts enriched in peptides.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Inhibition of HIV-1 by Cyclotide-Enriched Extracts of Viola tricolor

et al. 2022

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Since viral infectious diseases continue to be a global health threat, new antiviral drugs are urgently needed. A unique class of therapeutic compounds are antimicrobial peptides (AMPs). They can be found in humans, bacteria and plants. Plants express a wide variety of such defense peptides as part of their innate immune system to protect from invading pathogens. Cyclotides are non-classical AMPs that share a similar structure. Their unique topology consists of a circular peptide backbone and disulfide bonds. In previous studies they have been attributed to a wide range of biological activities. To identify novel cyclotides with antiviral activity, we established a library of plant extracts largely consisting of cyclotide-rich species and screened them as inhibitors of HIV-1 infection. Subsequent extraction and fractionation revealed four cyclotide-containing subfractions from Viola tricolor with antiviral activity. These subfractions inhibited HIV-1 infection with IC50 values between 0.6 and 11.2 μg/ml, and selectivity indices of up to 8.1. The identification and characterization of antiviral cyclotides and the determination of the antiviral mechanisms may allow to develop novel agents to combat viral infections. Therefore, cyclotides represent a natural source of bioactive molecules with prospects for development as therapeutics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro Inhibition of HIV-1 by Cyclotide-Enriched Extracts of Viola tricolor

et al. 2022

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“…Besides cucurbitacins, which are the most significant compounds presented in B. alba extract, other compounds in the extract, such as lectins, flavonoids, and lipids, also act synergistically to increase the biological activity [44]. Previous studies showed the significance of cucurbitacins isolated from B. alba, particularly cucurbitacins B, D, E, I, and their derivatives, owing to their therapeutic effect or adjuvant management [45][46][47]. Cucurbitacins J, K, and L are found in significant amounts in the roots [41,48] and exert cytotoxic effects [41].…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

et al. 2019

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Bryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

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“…Traditional medicine states its use for antiinflammatory, antibacterial, laxative‐purgative and smooth muscle relaxant properties, that appear to be due to the content in active principles (especially cucurbitacins) . It is known as a toxic species, but despite this fact, recent scientific sources confirm its promising therapeutical potential …”

Section: Introductionmentioning

confidence: 99%

“…[11] It is known as a toxic species, [12] but despite this fact, recent scientific sources confirm its promising therapeutical potential. [1,13] There is little evidence about the content of flavonoids in this species, as most of the studies are focused on its content of cucurbitacins, that seem to be found mainly in roots. [11,14] Previous reports suggest the presence of saponarin, vitexin, isovitexin [15] and lutonarin [16] in the composition of the aerial parts of B. alba L. To the best of our knowledge, these are the only scientific reports on the flavonoid composition of this species.…”

Section: Introductionmentioning

confidence: 99%

Flavonoid composition, cellular antioxidant activity and (myelo)peroxidase inhibition of a Bryonia alba L. (Cucurbitaceae) leaves extract

Ielciu

Mouithys‐Mickalad

Franck

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives The aim of the present study consisted in the isolation of flavonoids from the leaves of Bryonia alba L. and evaluation of their antioxidant activity and inhibition on peroxidase‐catalysed reactions. Methods Flavonoids were isolated by preparative HPLC‐DAD and their structures were elucidated by MS and NMR. Inhibitory effect was tested by the horseradish peroxidase and the myeloperoxidase assays. Cellular antioxidant assays consisted in testing the inhibitory activity on the reactive oxygen species released upon activation of neutrophils freshly isolated ex vivo from equine blood and of human monocytes‐derived macrophages in vitro. Whole organism toxicity was assessed on zebrafish larvae. Key findings Four flavonoids (lutonarin, saponarin, isoorientin and isovitexin) were isolated. The performed assays showed significant antioxidant activity and inhibition for the peroxidase‐catalysed reactions. Absence of cellular and zebrafish toxicity was confirmed. Conclusions Bryonia alba L. leaves are particularly interesting for their flavonoids content and showed significant inhibitory effect on peroxidase‐catalysed oxidation of substrates (Amplex Red and L012), as well as antioxidant/antiradical activity, proving that this species has a medicinal potential. Moreover, the present study highlights the absence of the toxicity of these leaves and offers though a novel perspective on the species, previously known as being toxic.

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HPLC Determination of Free and Bound Amino Acids in Bryonia alba

Cited by 6 publications

References 2 publications

In vitro Inhibition of HIV-1 by Cyclotide-Enriched Extracts of Viola tricolor

In vitro Inhibition of HIV-1 by Cyclotide-Enriched Extracts of Viola tricolor

Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

Flavonoid composition, cellular antioxidant activity and (myelo)peroxidase inhibition of a Bryonia alba L. (Cucurbitaceae) leaves extract

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