HPLC‐DAD method for the detection of five annopurpuricins in root samples ofAnnona purpurea

Abstract

Introduction Annona purpurea is a species known in Mexico as “cabeza de negro”. In folk medicine A. purpurea root is used to treat patients with kidney diseases and cancer. Our recent studies demonstrated that this species contains five acetogenins named annopurpuricins A–E, which are active against tumoural cell lines in a subnanomolar range. Objective To develop an analytical method using a high‐performance liquid chromatography diode array detector (HPLC‐DAD) to quantify annopurpuricins A–E in different A. purpurea root samples. Methodology To quantify the five annopurpuricins A–E a sample treatment was carried out, which consisted of fractionation by means of cold and hot maceration; using solvents of ascending polarity: hexane, dichloromethane, methanol and water. The resulting extracts were subject to HPLC‐DAD analysis. The optimised chromatographic separation on a XBRIDGE C18 column achieved separation of all compounds in around 30 min. Results The developed method was validated according to ICH (International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use) validation guide. The developed analytical method was found fast, economic, robust, sensitive, linear and precise. The dichloromethane extract of A. purpurea contains annopurpuricin A in quantities 2‐ to 25‐fold higher than annopurpuricins B–E. This optimised method identified and quantified five annopurpuricins, highly bioactive molecules, in A. purpurea root. Conclusions The fingerprint of the dichloromethane extracts of A. purpurea was obtained at 210 nm. The results analysis allowed to quantify annopurpuricins A–E that are present in different collection batches of medium polarity extracts. After data analysis, annopurpuricin A could be establish as the metabolite marker of the root of the species.

Annona muricata Leaves as a Source of Bioactive Compounds: Extraction and Quantification Using Ultrasound

“…Different letters by column indicate significant statistical differences between treatments (α = 0.05). 1 The values were predicted using secondary polynomial equations, R 2 = 0.886.…”

“…ND = not detected. 1 UAE Optimal conditions: 4.54 min, 0.68 s pulse-cycle, 79.99% sonication amplitude. 2 Decoction: Fresh leaves in boiling water for 10 min.…”

“…Annona muricata L. is a native tree of America that has stirred great interest due to its various therapeutic effects. In traditional medicine, bark, fruit, seed, and leaves have been used to treat fever, pain, headache, insomnia, rheumatism, parasitic and bacterial infections, hypertension, inflammation, diabetes, cancer, respiratory, and skin diseases [1]. In native regions, infusions from dry leaves and decoctions from fresh leaves are used as sedatives for chronic diarrhea, dysentery, hypertension, kidney, and biliary ailments.…”

Chemical comparison and discrimination of two plant sources of Angelicae dahuricae Radix, Angelica dahurica and Angelica dahurica var. formosana, by HPLC‐Q/TOF‐MS and quantitative analysis of multiple components by a single marker

“…Most (10), xanthotoxin (11), bergapten (14), pabulenol (16), oxypeucedanin (18), imperatorin (19), isoimperatorin (23), bergamottin ( 25) and 8-geranoxypsoralen (30), were identified in BZ and HBZ samples.…”

“…[18][19][20] Among these methods, HPLC is the most common and convenient method, which has been widely accepted and applied for the multi-component quantitative analysis and quality control of herbal medicines. 22,23 However, some reference standards are…”