HPLC–DAD determination of opioids, cocaine and their metabolites in plasma

Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac

“…Mor for brain and blood dialysate at S/N ratio of 3 were 0.4 ng/ml (2.7 fmol on column) and 0.6 ng/ml (3.8 fmol on column), respectively. The proposed method could improve the sensitivity of Mor by more than 20 times compared to HPLC-DAD method [7,8] and native FL method [13][14][15]. Furthermore, it was comparable to those of HPLC-ECD [10] and -MS/MS method [20] published recently for determination of Mor in microdialysate.…”

“…For the determination of Mor, high performance liquid chromatography (HPLC) methods with UV or DAD detection [7,8], electrochemical detection (ECD) [9,10], mass spectrometry (MS) detection [11,12] and fluorescence detection (FL) [13][14][15][16] are widely used. Quite recently, HPLC methods combined with tandem MS/MS for very low-level quantification of Mor in biological samples have been described with sub-nanogram per milliliter of quantitation limit [17][18][19][20].…”

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

“…HPLC with UV detection is a common analytical method for these drugs in biological samples. However, many reports describe the need for multiple extractions and complex chromatographic systems to ensure reproducibility and resolution [19][20][21][22][23][24][25]. Recently, a method employing HPLC combined with electro spray ionization-tandem mass spectrometry (HPLC-ESI-MS-MS) for low level quantitation of MOR, M3G and M6G has been described [26].…”

Análise do teor de cocaína em amostras apreendidas pela polícia utilizando-se a técnica de cromatografia liquida de alta eficiência com detector UV-Vis

“…Os efeitos iniciais da cocaína no organismo constituem em euforia, excitação, insônia e supressão do apetite, devido à ação no sistema nervoso central [4]. A análise de cocaína pelas técnicas de cromatografia líquida de alta eficiência (CLAE) [6][7][8][9] e cromatografia gasosa (CG) [10][11][12][13][14][15][16] tem sido amplamente reportada na literatura, indicando o potencial das mesmas para as análises forenses. A boa acuracidade e robustez, aliadas à possibilidade de obtenção de limites de detecção na ordem de ng mL -1 para a cocaína utilizando-se tais metodologias, possibilitou a aplicação destas técni-cas cromatográficas na análise do referido entorpecente em diversas matrizes, tais como fluidos biológicos [7,15,16], cabelo [6,8,14] e peças de interesse forense, como papel moeda [16], indicando a versatilidade de tais métodos.…”