HPLC and UV-spectrophotometry analysis of flavonoids in spray-dried and freez-dried extracts of Teucrium polium L. (Lamiaceae)

Stefkov, Gjoshe; Karapandzova, Marija; Стефова, Марина; Bankova, Vassya; Kulevanova, Svetlana

doi:10.33320/maced.pharm.bull.2012.58.005

Cited by 5 publications

(3 citation statements)

References 23 publications

(20 reference statements)

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“…The modified method described by Stefkov et al (2012) was used for separation of flavonoids. The gradient elution profile was as follows: 10% B 0-5 min, gradient 10%-60% B during 5-60 min, 60% B held for 5 min, then ramped from 60% to 90% B in 2-3 min, and equilibrated for further 5 min; mobile phases -A: water acidified with ortho-phosphoric acid, pH 3, B: acetonitrile.…”

Section: Phytochemical Analysis 241 High-performance Liquid Chromatography (Hplc) Analysis Of Polyphenolsmentioning

confidence: 99%

Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Milošević-Djordjević¹,

Jakovljević²,

Marković³

et al. 2018

Turk J Biol

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Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caffeic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium, except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly affect the nuclear division index (NDI), whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effects in PBLs.

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Section: Phytochemical Analysis 241 High-performance Liquid Chromatography (Hplc) Analysis Of Polyphenolsmentioning

confidence: 99%

Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Milošević-Djordjević¹,

Jakovljević²,

Marković³

et al. 2018

Turk J Biol

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“…The HPLC instrument used was GX-281 instrument with ODS HYPERSIL 250 mm with 5µ particle size. The analysis was done by reverse phase gradient elution mode based on a published method 18 . Scanning was at 348 nm, which is in the region where flavones exhibit maximum absorption.…”

Section: Screening For Diosmetin By Reverse Phase High Performance Liquid Chormatography (Rp-hplc)mentioning

confidence: 99%

Determination of the Total Polyphenolic Content and Screening for Diosmetin in Four Philippine Edible Plants

2020

IJGHC

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Four Philippine edible plants were analyzed for polyphenols and diosmetin. They were selected because they have been reported to contain diosmetin which is a non-widespread flavone and the active agent in some venoactive drugs for the treatment of varicose veins, hemorrhoids and other venous diseases. The plants analyzed were Citrofortunella microcarpa, Citrus aurantium Linn., Premna odorata Blanco and Luffa cylindrica Linn. The plant parts used were those that were not generally perceived to have much commercial value. The UV-Vis spectra at 431.5 nm of the DCM-soluble extracts showed the total polyphenolic content, expressed as quercetin, increases in the following order: P. odorata, L. cylindrica, C. aurantium, C. microcarpa. The HPLC chromatograms at 348 nm of the DCM-soluble extracts showed the presence diosmetin in all plant extracts. The retention times were: 4.250 mins in the rind of C. aurantium, 4.103 mins in the rind of C. microcarpa, 4.129 mins in the leaves of P. odorata, and 4.297 mins in the leaves of L. cylindrica. The diosmetin content increases in the following order: L. cylindrica, P. odorata, C. aurantium, C. microcarpa. This study concludes that the rind of the widely cultivated C. microcarpa, which are generally thrown as garbage, can be a very good source of polyphenolics, particularly the nonwidespread, venoactive and chemopreventive diosmetin. This abundant, but seemingly useless, material can be commercialized as dietary supplement.

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“…The HPLC method, modified from the methods of Stefkov et al, 27 was used for the separation of phenolic acid and flavonoids in A. vulgaris and A. alba extracts. The HPLC system (Shimadzu, Kyoto, Japan) consisted of degasser DGU-20A3, analytical pumps LC-20AT, 7125 injector and SPD-M20A diode array detector, and CBM-20A system controller.…”

Section: Separation Of the Ethyl Acetate Extracts Of A Vulgaris And mentioning

confidence: 99%

Ethyl Acetate Extracts of Two Artemisia Species: Analyses of Phenolic Profile and Anticancer Activities Against SW-480 Colon Cancer Cells

Jakovljević

Grujičić

Živanović

et al. 2019

Natural Product Communications

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Because Artemisia vulgaris L. and Artemisia alba Turra are traditional medicinal plants used for the treatment of different diseases, we evaluated the cytotoxic/apoptotic activity of ethyl acetate extracts from these natural products against human colon cancer cells SW-480. The extracts contained a large amount of the total polyphenols and flavonoids. The phenolic profile showed the presence of phenolic acids (gallic, p-coumaric, vanillic, and ferulic acids) and flavonoids (rutin, myricetin, luteolin, quercetin, and apigenin). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay indicated that antiproliferative activities of both A. vulgaris and A. alba extracts increased with the extension of time exposure, with decreasing IC 50 values. Mitomycin C (MMC) alone had antiproliferative activity, but in combination with plant extracts caused stronger effect with lower IC 50 values. Flow cytometry analyses showed that A. alba extract induced higher percentage of SW-480 cells in the early stage of apoptosis (33.5 ± 1.6 vs 0.7 ± 0.1, P < 0.05), whereas the A. vulgaris extract significantly increased the percentage of cells in necrosis (82.4 ± 5.0 vs 53.9 ± 2.3, P < 0.05). In conclusion, A. alba extract can be considered a potential source of bioactive components with anticancer activity or be used as a dietary food supplement or supplement to chemotherapy due to its synergistic effect with the MMC.

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HPLC and UV-spectrophotometry analysis of flavonoids in spray-dried and freez-dried extracts of Teucrium polium L. (Lamiaceae)

Cited by 5 publications

References 23 publications

Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Determination of the Total Polyphenolic Content and Screening for Diosmetin in Four Philippine Edible Plants

Ethyl Acetate Extracts of Two Artemisia Species: Analyses of Phenolic Profile and Anticancer Activities Against SW-480 Colon Cancer Cells

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