Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes

Abstract

Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caffeic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium, except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly affect the nuclear division index (NDI), whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effects in PBLs.

Protective effect of extracts of Teucrium Polium and Rumex Crispus against cyclophosphamide-induced genotoxic damage in human lymphocytes

“…For cancer therapy, to determine the potential anticancer effects of T. polium, all these in vitro studies showed the essentiality of the animal experimentation and clinical trials. T. polium contains five phenolic acids and six flavonoids, the most important therapeutic polyphenolic compounds [9,31]. In our study, moderate and high concentrations of plant extractions showed a protective effect for many genotoxicity tests.…”

“…Quercetin-3-O-β-D-glucuronopyranoside (QGC) that has anti-oxidative, antitumor and anti-inflammatory effects in vivo, the most important flavonoid glucoside extracted from Teucrium and Rumex species [7,9,28,29]. The antimutagenic and anticancerogenic effects of T. polium were tested in the mammalian system and this plant extract was reported to decrease the SCE and chromosomal abnormalities [24].…”

“…In our study, moderate and high concentrations of plant extractions showed a protective effect for many genotoxicity tests. Numerous studies provide evidence for mutagenic/antimutagenic or prooxidant/antioxidant activities largely depend on the concentration used of medical plants [8,9,31,32].…”

“…Northwest Asia and the Mediterranean region have been used by the public for 2000 years for treatment of diabetes, convulsion and gastrointestinal inflammation [6][7][8]. The results of Milosevic-Djordjevic et al [9] who found that polyphenolic contents of T. polium by HPLC showed phenolic acid (gallic, vanillic, caffeic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Teucrium species have been reported to be able to repair DNA damage by stimulating deteoxification enzymes and have antioxidant, anticancer, hepatoprotective, hypolipidemic, hypoglycemic and antimicrobial effects [6,10,11].…”

Phytochemical Composition and Pharmacological Activities of Teucrium polium L. Collected from Eastern Turkey

“…Decoction and infusion of the aerial parts of T. polium were analyzed by LC-MS/MS and luteolin-glycosides, pelargonin, and fumaric acid were exhibited as main constituents [ 48 ]. Twelve phenolic compounds were detected in the methanol extract of T. polium , containing high levels of catechin, luteolin, apigenin, rutin, quercetin, and chlorogenic acid whereas in the methanolic extracts of T. scordium five phenolic compounds were identified such as catechin, rutin, vanillic and p -coumaric acids [ 49 ]. In another study, the polyphenolic profiles of four Teucrium species from Macedonia were investigated and one hydroxycinnamic acid, eight phenylethanoid glycosides, flavonoid aglycones, and glycosides were found in the extract [ 47 ].…”