HPLC Analysis of Human Urine for Oxalate Content

Abstract

Objective: In the present communication, development and validation of reverse phase-high performance liquid chromatography method have been carried out for estimation of oxalate content in the urine of human volunteers with recurrent kidney stone disease and healthy status. Methods:The analysis of oxalic acid has been carried out on KYA TECH HiQ Sil C18HS column using a mobile phase of methanol: 0.001 N acetic acid in water (50:50, v/v) with a flow rate of 1 ml/min and detection wavelength, 237 nm.Results: Analysis of oxalate content was carried out using single point calibration method with retention at 2.705 min with good resolution parameters. Urine sample collected from kidney stone patients and healthy volunteers over the period of 24 h were analyzed and it has been found that concentration of oxalate in healthy volunteers is less than 12 µg/ml whereas that in case of kidney stone patients is in the range of 39-151 µg/ml and this data can be utilized for further interpretations about oxalate content in healthy and kidney stone diseased volunteers. This method was validated as per united states food and drug administration (USFDA) guidelines by the study of accuracy, precision, linearity, range, selectivity, the lower limit of quantitation, extraction recovery studies and stability studies for determining oxalate content in the urine of human volunteers. As relative standard deviations of oxalate content estimated are less than 5 percent, the method can be claimed accurate, precise, sensitive and selective for determining oxalate content in the urine of human volunteers. Conclusion:The results are satisfactory, proving the effectiveness of the method for analysis of oxalate content from other biological fluids with few optimizations.

Solid Electrochemiluminescence Sensor by Immobilization of Emitter Ruthenium(II)tris(bipyridine) in Bipolar Silica Nanochannel Film for Sensitive Detection of Oxalate in Serum and Urine

“…Therefore, the detection of oxalate ions in blood and urine is of great significance for monitoring renal function, assessing the risk of cardiovascular diseases, diagnosing gout, and detecting urinary stone formation. Currently available methods for oxalate ion detection include enzymatic methods [ 12 ], high-performance liquid chromatography (HPLC) [ 13 ], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [ 14 , 15 ], electrochemical methods [ 16 ], fluorescence spectroscopy [ 17 ], ultraviolet-visible spectroscopy (UV-Vis) [ 18 ], and capillary electrophoresis (CE) [ 19 ]. Enzymatic analysis exhibits high selectivity but is limited by the susceptibility of enzyme activity to environmental factors.…”

Potency of Chives (Allium Schoenoprasum L.) Leaves Infuse as Inhibitor Calcium Lithogenesis on Urinary Tract

“…The problems related with kidney are the major problem for human beings. The major functions of the kidney are balancing of electrolytes and regulation of water, production of erythrocytes, regulating the acidbase balance, regulation of blood calcium level, and involve the process of gluconeogenesis [1][2][3]. There will be many diseases which can affect the kidney function.…”

Development of novel point-of-care-testing for oxalate detection in urine based on oxalate-binding ligands

“…The non-enzymatic techniques for urinary oxalate measurement include high performance liquid chromatography (HPLC) [12], capillary electrophoresis (CE) [13], ion chromatography [14] and gas chromatography [15]. In our research group, we recently developed a new method for indirectly detecting oxalate in the urine based on precipitation of CaOx crystals, called calcium oxalate crystallization index (COCI) [16].…”

“…High-performance liquid chromatography (HPLC); There are many studies demonstrated measurement of oxalate by HPLC technique. One of them is study by Suryavanshi et al [12]. They used reverse phase-HPLC for oxalate analysis.…”

“…They used reverse phase-HPLC for oxalate analysis. The detecting wavelength for oxalate is at 237 nm, and oxalate concentration is calculated from area under peak (Figure 8) obtained from the chromatogram (Figure 9) They found that the limit of detection of this method is of 49 ng/mL [12]. Figure 9.…”