HPLC Analysis of Amino Acid and Antioxidant Composition of Three Medicinal Plants of (Pithoragarh) Uttarakhand Himalayas

Abstract

Antioxidant phytochemicals such as vitamin C, β-carotene, lutein, α-tocopherol, amino acid and total phenolics, were estimated in some medicinal plants using a reverse phase HPLC system. Wild plants play a vital role in the health security of Himalayan inhabitants and constitute significantly to their diet. Amino acid was analysis performed using the Waters Associates PICO-TAG method. The total amino acid content in P. indica was 58.80mg amino acid/g sample (dry weight), E. thymifolia was 123.92mg amino acid/g sample (dry weight) and P. hirta was 225.73mg amino acid/g sample (dry weight). The total essential amino acids in P. indica, E. thymifolia and P. hirta were 33.58, 57.99 and 145.82mg amino acid/g respectively. Among the three investigated plants, Carotenoids viz xanthophylls content was found 0.13 to 151.01 mg/100g dry weight basis. The maximum xanthophylls content was found in P. indica leaves and minimum in P. hirta rhizomes. The β-carotene content varies from 4.62 -374.55mg/100g on dry weight basis. DL-α-tocopherol in these medicinal plants was found 13.48mg/100g, 24.95mg/100g and 9.13mg/100g on dry weight basis in P. hirta, E. thymifolia and P. indica respectively. This study discovers amino acid and antioxidant content in medicinal plants. Such information will increase the understanding of the faction of these antioxidant phytochemical in lowering incidence of ageing and other chronic diseases. Results of our study suggest the great value of these species for use in pharmacy and phytotherapy. Based on this information, it could be concluded that this plant is natural sources of antioxidant substances of high importance.

Bioactive Compounds and Antiradical Activity of the Rosa canina L. Leaf and Twig Extracts

“…The total amino acid contents were 58.80 mg g −1 DW in P. indica, 123.92 mg g −1 DW in E. thymifolia and 225.73 mg g −1 DW in P. hirta. The ratios of essential amino acids to total amino acids were 0.57, 0.47 and 0.65, respectively [52]. Although in our study the leaf extract was richer in amino acids, the ratio of essential to total amino acids was 0.37, whereas in the twig extract it was 0.55.…”

Variation of Minerals Content and Some Bioactive Compound in Some Wild and Cultivated Edible Plants Grown Naturally in Kastamonu Region

“…In another study, Guzelsoy et al (2017) reported that some wild edible plants collected from three different biogeographic of Turkey are rich in vitamin C, ranging from 2.0 to 129.4 mg 100g -1 . Prasad and Chandra (2018) also reported similar values of ascorbic acid in some wild edible medicinal herbs as P. hirta (108.40 mg 100g -1 ), E. thymifolia (88.48 mg 100g -1 ), and P. indica (77.49 mg 100g -1 ). According to the results, the daily vitamin C requirement of an adult person can be provided by consuming both the T. orientalis and T. reticulatus.…”

“…Similarly, in a study carried out by Moran-Palacio et al (2014), some medicinal species used in traditional medicine were found to be very rich in amino acids. Prasad and Chandra (2018) showed that the total essential amino acid levels were ranging between 35.58 mg 100g -1 and 145.82 mg 100g -1 , and the amount of total amino acid content of Pavetta indica was 58.80 mg 100g -1 , E. thymifolia 123.92 mg g -1 , and P. hirta 225.73 mg 100g -1 .…”

Prophylaxis of malaria by Artemisia infusions: The key role of lysine

“…14 And that the medicinal plants (dry) leaves. Euphorbia thymifolia only contains 0.14 g/100g of lysine, Zinziber officinale 0.14 g/100g and Pavetta indica 0,30 g/100 g 15 And for Vernonia amygdalina 0.05 g/100g.were found. All values lower than for Artemisia.…”