HPLC analysis, cytotoxicity, and safety study ofMoringa oleiferaLam. (wild type) leaf extract

Abstract

The study was planned to evaluate toxicity of M. oleifera leaf methanol extract in Wistar rats, cytotoxic potential and chemically characterize it. Acute toxicity study revealed no mortality at 2,000 mg/kg dose. In subchronic toxicity, 150, 300, and 600 mg/kg extract were administered in both sexes for 90 days. A decrease in body weight, cholesterol, and low-density lipoproteins, as well as an increase in the platelet count were observed. The histology of heart, lung, and kidney was normal. The oxidative stress biomarkers were normal in the liver tissue. In vitro cytotoxicity assay revealed that IC50 of the plant extract was more than 1,000 µg/ml. FTIR explored various functional groups and HPLC analysis indicated the presence of kaempferol and quercetin in the plant extract. These results showed that the plant extract might be safe up to 2,000 mg/kg single dose. The long term use of the plant extract was mostly devoid of major system toxicities. Practical applications M. oleifera is a multipurpose tree. It is a cruciferous plant that has nutritional as well as medicinal properties. It is exhibited a multitude of nutraceutical or pharmacological properties such as anti-inflammatory, lipid-lowering, anticancer, hypoglycemic, and antihypertensive activities. It is widely used by local inhabitants for their health, food, agriculture, and cosmetic purposes. Among other plant parts, its leaves are mostly used as a functional food because of abundant proteins, β-carotene, polyphenol, vitamin C, and vitamin E. The current study was designed to validate its safety in acute and long term use. The data of this study suggested that the plant can be safely used as a nutraceutical as well as a nutritional food.

Nutritional Value of Moringa oleifera Lam. Leaf Powder Extracts and Their Neuroprotective Effects via Antioxidative and Mitochondrial Regulation

“…Additionally, Nair et al (2020) have recently demonstrated that compounds presented in moringa extracts are able to pass across Blood-brain-barrier (BBB) and to reach its pharmacological target [ 55 ]. Regarding the safe toxicological profile of moringa, doses of up to 2000 mg/kg of methanol and aqueous leaf extract were safe in animal studies [ 56 , 57 ].…”

Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

“…It attenuated the hematopoietic changes, hepatic and renal dysfunction, MDA formation, and DNA fragmentation induced by TMX. Saleem et al [ 62 ] confirmed the safety of MLE, showing that the acute and sub-chronic administration of MLE to Wistar rats did not appear to result in notable abnormalities in organ relative weights, or hematological and biochemical parameters. The findings of several studies also agreed with our results.…”

Extraction and Optimization of Active Metabolites From Cluster Bean: An In Vitro Biological and Phytochemical Investigation

“…Six solvent extracts of cluster bean were used for reverse-phase HPLC to quantify the phenolic and flavonoids contents ( Proestos et al, 2005 ). Reaction mixture of each extract was prepared in methanol and filtered through .2 μm syringe membrane filters ( Saleem et al, 2020 ) before injecting into HPLC. The C18 column (250 × 4.6 mm internal diameter) of 5 μm film thickness was used with temperature control system set at 30°C.…”