Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

Elhamalawy, Osama H.; Al-Anany, Fathia S.; Makawy, Aida I. El

doi:10.1016/j.toxrep.2021.12.012

Cited by 12 publications

(7 citation statements)

References 55 publications

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“…Present data revealed a significant increase in the mitochondrial DNA 8-OH-2 DG of MX treated rat brain. Our result is consistent with the findings of Elhamalawy et al (2022) who posted that MX significantly rises the DNA damage rates in hepatic and renal cells of treated mice as measured by diphenylamine test and comet assay. MX directly interacts with DNA, and has a significant impact on numerous biological and biochemical indices in treated animals Jameel et al (2020).…”

Section: Discussionsupporting

confidence: 93%

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Abdel-Razik

Mosallam

Hamed

2022

Alexandria Science Exchange Journal

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Mitochondria sustain healthy brain function. Herein we aimed to evaluate the thiamethoxam (MX) effect on the rat brain mitochondria in addition to the protective role of N-acetylcysteine (NAC) against MX harmful effects. Thiamethoxam was administered orally with five doses each week for 28 days to male albino rats at 1/50 of the LD50 (31.26 mg/kg bw). The results demonstrated that the thiamethoxam neurotoxicity was confirmed by the significant rising in acetylcholinesterase, and lactate dehydrogenase activities of plasma. A significant increase in mitochondrial antioxidants as superoxide dismutase and reduced glutathione was found. Also, significant induction of the oxidative stress and DNA damage via rising the malondialdehyde, and 8-hydroxy-2'-deoxyguanosine biomarkers was recorded by 32.5% and 118.61% respectively. Substantial depression in mitochondrial NADH dehydrogenase, cytochrome c reductase, cytochrome c oxidase, and Mg 2+ ATPase complexes as well as 23 % cerebral infarction was manifested by histological evaluation using the dehydrogenase activity indicator, 2, 3, 5-triphenyl tetrazolium chloride staining. In conclusion, MX can pose a hazard to the integrity and functioning of rats' brain mitochondria, perhaps leading to neurodegenerative disorders. Additionally, earlier treatment of the synthetic antioxidant N-acetylcysteine could prove beneficial in combating the harmful effects of thiamethoxam.

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Section: Discussionsupporting

confidence: 93%

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Abdel-Razik

Mosallam

Hamed

2022

Alexandria Science Exchange Journal

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“…TMX has a strong insecticidal action on a wide range of economically important pests. On the other hand, the administration of TMX to experimental animals had a significant negative impact on the function of several organs [16]. Oral administration of TMX reduced plasma cholesterol levels, an early indicator of liver failure that is likely linked to future histopathological alterations, and boost protooncogenes, induction of apoptosis, and tumor progression in mice [17][18].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system

et al. 2022

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Neonicotinoid insecticides usage is currently widespread, but this poses a challenge when considering the potential for occupational and environmental contamination. One of the most extensively used insecticides is thiamethoxam (TMX), a second-generation neonicotinoid insecticide. This study aimed to see if sub-lethal dosages of TMX insecticide had any negative impacts on epididymal sperm parameters, serum hormones, oxidative status, and testicular histology. The experimental cohorts were given a low dose of TMX (156 mg/kg bw), a high dose of TMX (312 mg/kg bw), or an untreated control for eight weeks. The sperm count, percent of viability, motility, and motility progressing and fructose level significantly decreased in both TMX-treated groups compared to the control group. Furthermore, TMX administration induced sperm morphological defects, serum hormone disturbances as significant reduction testosterone level, oxidant/antioxidant status imbalance as significant decline in catalase (CAT) and glutagthione peroxidase (GSH) content and significant rise in malondialdehyde (MDA) level and testicular histopathological alternation. TMX caused significant increase DNA damage in testicular tissue represented in tail DNA percent, comet percent, comet length, tail moment, and Olive moment. In conclusion, TMX exposure may have a deleterious impact on male albino rats' fertility through spermatogenesis, steroidogenesis and testicular redox status disruption, and testicles DNA impairment.

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“…Thiamethoxam administration (87.73 mg/kg b.w. for 28 days) on male Swiss albino mice produced significant reductions in erythrocyte count and hemoglobin concentration [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Ecotoxicological Studies on the Action of Actara 25 WG Insecticide on Prussian Carp (Carassius gibelio) and Marsh Frog (Pelophylax ridibundus)

Păunescu

Soare

Fierăscu

et al. 2022

Toxics

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The toxic action of the Actara 25 WG insecticide (it contains 25% thiamethoxam as an active substance) in non-lethal doses was studied in two species of aquatic organisms—the Prussian carp (Carassius gibelio) and the marsh frog (Pelophylax ridibundus)—at two thermal levels, 6–8 °C (low temperature) and 18–20 °C (room temperature), respectively. In the Prussian carp, we recorded decreases in oxygen consumption and stimulation of the respiratory rhythm, changes that were more pronounced in the case of intoxicated fish and when the species were kept at room temperature. The histopathology of the lung in the frog illustrated the thickening of the conjunctival septum, an increase in the number of mucous cells, and an increase in the ratio between the diameter of the nucleus and the diameter of the pneumocyte. All of these changes were more pronounced in the animals kept at higher temperature. Our study looks at the extent to which temperature changes can influence the ability of poikilothermic organisms to withstand the presence of toxic substances in the environment as a result of the impact of the use of insecticides in agriculture. The two tested organisms are a common presence for the study area, which was affected in the last decade by climate change.

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Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

Abstract: Graphical abstract

Cited by 12 publications

References 55 publications

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system

Ecotoxicological Studies on the Action of Actara 25 WG Insecticide on Prussian Carp (Carassius gibelio) and Marsh Frog (Pelophylax ridibundus)

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