HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

Abstract

Research ArticleHPLC analysis, anti HPLC analysis, anti HPLC analysis, anti---oxidant activity oxidant activity oxidant activity of of of Genista ferox Genista ferox Genista ferox and its anti and its anti and its anti---proliferative effect proliferative effect proliferative effect in in in HeLa HeLa HeLa cell cell cell line line line BJP IntroductionPlants used in ancient times as medicines to alleviate symptoms of various ailments (Saeed et al., 2012). In spite of the great progress in modern medicine in recent decades, but the herbal medicine still make an important contribution in the health care. Many medicinal and aromatic plants contain huge amounts of antioxidants such as polyphenols. These substances can have an important role in the absorption and neutralize free radicals, and the extinction of the shirt and triple oxygen or peroxide decomposing. Many of these phytochemicals have anti-oxidant capabilities so they contribute significantly to the fight against many human diseases and thus contribute to the reduction of mortality (Djeridane et al., 2006). Phenolic compounds such as flavonoids and phenolic acids have different biological effects, such as the effects of anti-atherosclerotic, anti-inflammatory and anti-cancer, as a result of the anti-oxidant activity (Krishnaiah et al., 2011).The Fabaceae family contains approximately 700 genera, in Algeria there are about 53 genera and 337 species (Quezel and Santa, 1963). Genista genus has about 150 species in Europe and the Mediterranean region (RE, 1987). A literature survey shows that this genus is a good source of phenolic compounds, in particular isoflavonoids, which are known for their diverse biological activities. The recent studies on the species of the Genista genus showed pharmacological interest (Belle et al., 1995;Herrera et al., 1992).In the present study, the qualitative and quantitative analysis, the identification and quantification of phenolic acids and flavonoids in chloroform, ethyl AbstractThe prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant activity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by Folin-Ciocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 -308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 -124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against H...

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

“…It is reported that Fabaceae family rich in phenolics, such as gallic acid, fumaric acid, chlorogenic acid, 4hydroxybenzoic acid, caffeic acid, cinnamic acid, pcoumaric acid, catechin derivatives, quercetin, hesperidin, taxifolin, naringenin, myricetin, resveratrol, apigenin, and galangin (Sobeh et al, 2016;Bencherchar et al, 2017). Our phenolic composition results are not exactly compatible with those from previous studies.…”

“…Anticancer properties of phenolics may derive from their ability to increase apoptosis and the cell cycle arrest, inhibit DNA synthesis, and modulate signal transduction pathways (Demir et al, 2017b;Demir et al, 2018b;Oyenihi and Smith, 2019). In both our characterization studies and other previous studies, Fabaceae species have been shown to be rich in phenolics, such as gallic acid, fumaric acid, chlorogenic acid, 4-hydroxybenzoic acid, caffeic acid, cinnamic acid, p-coumaric acid, catechin derivatives, quercetin, hesperidin, taxifolin, naringenin, myricetin, resveratrol, apigenin, and galangin (Sobeh et al, 2016;Bencherchar et al, 2017). There are many studies on the anticancer effects of these phenolic compounds on various cancer cells (Di Domenico et al, 2012;Oyenihi and Smith, 2019).…”

Chemical analysis of phenolic compounds and determination of antioxidant, antimicrobial and cytotoxic activities of organic extracts of Pinus coulteri

“…Other studies reported that the use of natural anti-oxidants can reduce the risk of various cancers, an effect seemingly mediated by phenolic compounds (Bencherchar et al, 2017). Thus, the presence of cytotoxic agents in plants belonging to Pinaceae family is demonstrated (Cui et al, 2005;Huseini et al, 2015).…”

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

“…Members of Fabaceae family have been used for many years in traditional medicine to treat rheumatism, arthritis, inflammation, neoplasm, hemorrhoid, bronchitis, asthma, urinary tract infections, and liver diseases (Bremner et al, 2009;Lacerda et al, 2014;Kumar et al, 2017). It is reported that Fabaceae family has rich content of phenolic acids and flavonoids (Sobeh et al, 2016;Bencherchar et al, 2017). The genus Dorycnium belongs to the family Fabaceae (Leguminosae) and is widely distributed in Europe and Asia (Stefanović et al, 2015;Kocabas and Ilcim, 2016).…”