HPLC Analysis and Standardization of Arjunarishta – An Ayurvedic Cardioprotective Formulation

Abstract

Arjunarishta (Parthadyarishta) is an important Ayurvedic formulation used for cardiovascular disorders and is prepared by fermenting the decoction of specified plant materials using flowers of Woodfordia fruticosa. In present communication, an HPLC-PDA method was developed for the standardization of Arjunarishta by quantitative estimation of major antioxidant compounds, ellagic acid, gallic acid, ethyl gallate, quercetin and kaempferol as markers. The developed method was validated with respect to linearity, precision, accuracy, and robustness. The HPLC analysis showed an increase in amount of ellagic acid and gallic acid during preparation, i.e. decoction vs formulation. A similar increase in free radical scavenging activity of formulation vs decoction was also observed. Arjunolic acid and arjunic acid were not detected in the formulation.

Applications of High Performance Liquid Chromatography in the Analysis of Herbal Products

“…Lal et al [34] reported an HPLC-PDA-based standardization method for this formulation, utilizing more than one marker compound. It is prepared by fermenting the decoction of the bark of Terminalia arjuna, fruits of Vitis vinifera, and flowers of Madhuca indica using flowers of Woodfordia fruticosa.…”

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“…It was already reported in literature that several factors such as generation of acid and alcohol during fermentation of decoction stimulate the hydrolysis of the process, thus causing biotransformation of chemical substances. [13,14] Various reports have been published which explained the degradation pattern of polyphenolics substances during fermentation. [15] Publication of these data promoted us to explore the effect of fermentation on chemical changes in V. vinifera fruits.…”

Ethyl gallate isolated from Pistacia integerrima Linn. inhibits cell adhesion molecules by blocking AP-1 transcription factor

“…2C). Structure of EG was further validated by comparing the spectral data from earlier studies (He et al, 2008;Lal et al, 2009).…”