HPLC analysis and antidiabetic effect of Rheum ribes root in type 2 diabetic patients

Abstract

Background and objective: Rheum ribes (polygonaceae) roots are used traditionally to treat diabetes, hypertension, obesity, ulcer, diarrhea, anthelmintic and expectorant. The purpose of this study was to investigate clinically the antidiabetic activity of Rheum ribes in type 2 diabetes mellitus patients and phytochemical study for the correlation of antidiabetic activity with the active constituents in the plant. Methods: The antidiabetic activity of Rheumribes given alone and in combination with standard oral hypoglycemic agents was investigated in type 2 diabetes mellitus. The study was conducted on 120 patientswith type 2 diabetes mellitus for a period of 12 weeks. Preliminary phytochemical screening was carried out followed by high performance liquid chromatography analysis for the identification of flavonoid constituents in the root of Rheumribes. Results: Rheumribes root showed significant blood glucose reduction (P <0.01) on 12 th weeks of the observation period with 39.63% percent blood glucose reduction. The combination treatment of Rheumribes root and glibenclamide showed a significant difference (P <0.05) with the treatment group of glibenclamide and metforminwith maximum percent of reduction in blood glucose level of 48.91%.The results of preliminary phytochemical screening showed the presence of alkaloids, tannins, flavonoids, anthraquinones, and quinones. Quercetin was identified by high performance liquid chromatography. The proposed high performance liquid chromatography method was validated for linearity, accuracy, precision and limit of quantitation. Conclusion: Rheum ribes root was found to reduce significantly blood glucose levels in type 2 diabetes mellitus patients.Quercetin was identified by high performance liquid chromatography as an important flavonoid constituent.

Effect of Rheum Ribes and Urtica Dioica on type 2 diabetic rats

“…[17,19] Multiple studies have shown that R. Ribes roots have antidiabetic activity, including antihyperglycemic, insulin-releasing, and antiretinopathic activities. [20][21][22][23] Urtica Dioica (U. Dioica) is another medicinal plant, which belongs to the Urticaceae family. This plant is also famous for its use as a food ingredient and herbal drug.…”

Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

“…Four hundred grams of dried powdered leaves and seeds of M. oleifera were extracted through 1 L ethanol (Chem-Lab, Belgium) using an ultrasonic-assisted extractor (LUC-405, Korea) for 1hr at 40°C. After filtration and drying, the extract was redissolved in 10% ethanol and fractionated by diethyl ether (Chem-Lab, Belgium), and ethyl acetate (Chem-Lab, Belgium) [19,20]. The obtained fractions were stored at 4°C until their usage for phytochemical screening and evaluation of their biological activity.…”

The first report about genetic diversity analysis among endemic wild rhubarb (Rheum ribes L.) populations through iPBS markers

“…Only Rheum ribes L. is naturally occurring in Turkey (Tosun and Kizilay, 2003) and it has also found in Iran, Pakistan, Afghanistan, Iraq, Armenia and Lebanon (Bazzaz et al, 2005;Ekincialp et al, 2019). It is perennial plant and its flower stalks are consumed as vegetables (Naemi et al, 2014); it could be used as a medicine for diabetes, root parts help to reduce glucose in the blood especially in type 2 diabetes (Raafat et al, 2014;Adham and Naqishbandi, 2015;Raafat and El-Lakany, 2018); and it has been reported as an effective herb in studies for diseases such as diarrhea, cancer, and Alzheimer's (Zahedi et al, 2015;Khiveh et al, 2017;Aygün et al, 2020).…”