HPAE-PAD - a sensitive method for the determination of carbohydrates

Jahnel, Jutta; Ilieva, Pavlinka; Frimmel, Fritz H.

doi:10.1007/s002160050819

Cited by 37 publications

(17 citation statements)

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“…Polymers were hydrolyzed at 123°C for 20 min in 2 M trifluoroacetic acid (modified from reference 2). Then the trifluoroacetic acid was evaporated, and the remaining sugars were dissolved in 1 ml of water and analyzed via high-performance anion-exchange chromatography with pulsed amperometric detection (27) using equipment from Dionex. Mixtures of the D-isomers of arabinose, fructose, fucose, galactose, glucose, mannose, ribose, and xylose were used as reference compounds.…”

Section: Methodsmentioning

confidence: 99%

Bacteria Associated with Benthic Diatoms from Lake Constance: Phylogeny and Influences on Diatom Growth and Secretion of Extracellular Polymeric Substances

Brückner

Bahulikar

Rahalkar

et al. 2008

Appl Environ Microbiol

125

128

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The composition of diatom-associated bacterial communities was studied with 14 different unialgal xenic diatom cultures isolated from freshwater epilithic biofilms of Lake Constance, Germany. A clear dominance of Alphaproteobacteria was observed, followed by Betaproteobacteria, Gammaproteobacteria, Bacteroidetes, and Verrucomicrobia. Pure cultures of the diatom Cymbella microcephala, which was found to be dominant in epilithic biofilms in Lake Constance, were cocultivated with six associated bacterial strains. All these bacterial strains were able to grow in C. microcephala cultures in the absence of organic cosubstrates. Diatom growth was generally enhanced in the presence of bacteria, and polysaccharide secretion was generally increased in the presence of Proteobacteria. The monomer composition of extracellular polysaccharides of C. microcephala changed in relation to the presence of different bacteria, but the dominant monomers were less affected. Our results indicate that these changes were caused by the diatom itself rather than by specific bacterial degradation. One Bacteroidetes strain strongly influenced carbohydrate secretion by the alga via extracellular soluble compounds. Biofilms were formed only in the presence of bacteria. Phylogenetic analysis and coculture studies indicate an adaptation of Proteobacteria and Bacteroidetes to the microenvironment created by the diatom biofilm.

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Section: Methodsmentioning

confidence: 99%

Bacteria Associated with Benthic Diatoms from Lake Constance: Phylogeny and Influences on Diatom Growth and Secretion of Extracellular Polymeric Substances

Brückner

Bahulikar

Rahalkar

et al. 2008

Appl Environ Microbiol

125

128

View full text Add to dashboard Cite

show abstract

“…Derivatives are generally formed before the individual sugars are separated chromatographically (e.g., Cowie and Hedges, 1984). Direct analysis of underivatized sugars using HPLC with pulsed amperometric detection has been used (e.g., Cheng and Kaplan, 2001;Jahnel et al, 1998;Mopper et al, 1992;Tranvik and Jørgensen, 1995).…”

Section: Sugarsmentioning

confidence: 99%