HOXA10 Regulates Expression of Cytokeratin 15 in Endometrial Epithelial Cytoskeletal Remodeling

Kallen, Amanda N.; Haines, Kaitlin; Taylor, Hugh S.

doi:10.1177/1933719113519168

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…Cytokeratins, constituents of intermediate filaments, are a primary determinant of epithelial cell shape [38]. The expression of cytokeratins has been observed to decrease during the implantation period in other species, such as goats and humans [39,40]. Thus, this decrease may be associated with either the loss or shortening of microvilli as porcine pregnancy progresses toward the time of attachment [41].…”

Section: Discussionmentioning

confidence: 92%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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Section: Discussionmentioning

confidence: 92%

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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“…The gene with the highest GS was HOXA10 while the gene with the highest MM was GGH. HOXA10 , is a homeobox gene that encodes a DNA-binding transcription factor that has been implicated in endometriosis [33], oncogenesis [34], and most recently in innate immune response regulation [35]. In a study of B cell differentiation, Yasmeen et al [36] demonstrated that the knockout of an aldehyde dehydrogenase-1 enzyme involved in retinol metabolism and retinoic acid synthesis and encoded by the ALDH1 family of genes resulted in increased expression of HOXA10 by downregulating the expression of the anti-inflammatory transcription factor peroxisome proliferator-activated receptor PPARG.…”

Section: Discussionmentioning

confidence: 99%

Network analysis of psoriasis reveals biological pathways and roles for coding and long non-coding RNAs

et al. 2016

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BackgroundPsoriasis is an immune-mediated, inflammatory disorder of the skin characterized by chronic inflammation and hyperproliferation of the epidermis. Differential expression analysis of microarray or RNA-seq data have shown that thousands of coding and non-coding genes are differentially expressed between psoriatic and healthy control skin. However, differential expression analysis may fail to detect perturbations in gene coexpression networks. Sensitive detection of such networks may provide additional insight into important disease-associated pathways. In this study, we applied weighted gene coexpression network analysis (WGCNA) on RNA-seq data from psoriasis patients and healthy controls.ResultsRNA-seq was performed on skin samples from 18 psoriasis patients (pre-treatment and post-treatment with the TNF-α inhibitor adalimumab) and 16 healthy controls, generating an average of 52.3 million 100-bp paired-end reads per sample. Using WGCNA, we identified 3 network modules that were significantly correlated with psoriasis and 6 network modules significantly correlated with biologic treatment, with only 16 % of the psoriasis-associated and 5 % of the treatment-associated coexpressed genes being identified by differential expression analysis. In a majority of these correlated modules, more than 50 % of coexpressed genes were long non-coding RNAs (lncRNA). Enrichment analysis of these correlated modules revealed that short-chain fatty acid metabolism and olfactory signaling are amongst the top pathways enriched for in modules associated with psoriasis, while regulation of leukocyte mediated cytotoxicity and regulation of cell killing are amongst the top pathways enriched for in modules associated with biologic treatment. A putative autoantigen, LL37, was coexpressed in the module most correlated with psoriasis.ConclusionsThis study has identified several networks of coding and non-coding genes associated with psoriasis and biologic drug treatment, including networks enriched for short-chain fatty acid metabolism and olfactory receptor activity, pathways that were not previously identified through differential expression analysis and may be dysregulated in psoriatic skin. As these networks are comprised mostly of non-coding genes, it is likely that non-coding genes play critical roles in the regulation of pathways involved in the pathogenesis of psoriasis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3188-y) contains supplementary material, which is available to authorized users.

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“…Reproductive Science has recently published several interesting articles on this field. 1,2…”

Section: The Endometrium and Implantationmentioning

confidence: 99%

“…Reproductive Science has recently published several interesting articles on this field. 1,2 In the current issue of Reproductive Sciences, Chen et al 3 take a step further into determining the dynamic players involved in the complicated physiological process of implantation from the endometrium perspective. With the aid of real-time quantitative polymerase chain reaction (PCR) and next-generation sequencing, the authors characterized the profile of messenger RNA (mRNA) and microRNA (miRNA) expression in the endometrium during the prereceptivity, receptivity, and implantation phases.…”

mentioning

confidence: 99%

In the Spotlight

Maduro¹

2015

Reprod. Sci.

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Implantation of the embryo is a multistage process crucial to a successful pregnancy that can only occur during a small window of opportunity. During this time, the blastocyst needs to be able to bind and invade the uterine wall. The endometrium, on the other hand, needs to be receptive to the invasion of the trophoblast. It has been estimated that about 75% of all concepti are lost before or at the time of implantation, and inadequate uterine receptivity is believed to account for two-thirds of implantation failures. Endometrium receptivity is therefore a topic of interest to the reproductive endocrinologist community, especially in light of unsuccessful in vitro fertilization cycles following the transfer of good embryos. Research has, therefore, focused on better understanding the molecular mechanisms that play a role at the time of implantation, both from the embryo and the endometrium side. Reproductive Science has recently published several interesting articles on this field. 1,2 In the current issue of Reproductive Sciences, Chen et al 3 take a step further into determining the dynamic players involved in the complicated physiological process of implantation from the endometrium perspective. With the aid of real-time quantitative polymerase chain reaction (PCR) and next-generation sequencing, the authors characterized the profile of messenger RNA (mRNA) and microRNA (miRNA) expression in the endometrium during the prereceptivity, receptivity, and implantation phases. Their results demonstrated that the genes mostly expressed at the implantation site are involved in providing energy for embryo implantation, producing functional protein to support embryo development, and trophoblast invasion. Of importance, the authors also verified that the tight regulation between miRNAs and mRNAs plays a major role in the endometrium receptivity preparation and maintenance of early pregnancy. Hub miRNAs (mmu-miR-96 and mmu-miR200b), in particular, were identified as upstream regulators of Bcl-2, klf13, and PGR.The characterization of the basic pathways involved in implantation leads to a better understanding of the whole process, potentially opening the doors to the discovery of new ways of improving the utero receptivity. Interestingly, however, oftentimes the learning process is turned around, with empirical results also bringing new clues into play. That has been the case with endometrial injury, which has proved to provide benefits to embryo implantation as discussed previously in Reproductive Sciences. 4 The underlying mechanisms to this process have, however, never been completely understood.In this same issue of Reproductive Sciences, Liao and colleagues 5 take a look at this favorable effect on embryo implantation through the establishment of a mouse model. The authors randomly allocated mice to either a control group (normal mating or mating with previous abdominal opening alone) or to an endometrial injury procedure protocol, where the animals were subjected to scratches with a blunt syringe on the right uterine...

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HOXA10 Regulates Expression of Cytokeratin 15 in Endometrial Epithelial Cytoskeletal Remodeling

Cited by 7 publications

References 20 publications

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Network analysis of psoriasis reveals biological pathways and roles for coding and long non-coding RNAs

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