How Useful is the AgNOR Staining Method for the Diagnosis of Salivary Gland Tumors?

Irani, Soussan; Zerehpoush, Farahnaz Bidari; Sabeti, Shahram

doi:10.17795/ajdr-25275

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…The AgNORs were numerous and pleomorphic in ACC. This finding is compatible with the study done by Irani et al, (2016), who detected significant differences between the type of tumor and AgNOR, with ACC having the highest AgNOR number (Irani et al, 2016). These findings contradict the findings of Adeyemi et al, study (2006), which showed no significant difference in their mean AgNOR value and different histological types.…”

Section: Discussionsupporting

confidence: 85%

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Hussien

Mohamed

Mansor

et al. 2022

Asian Pac J Cancer Prev

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Objective: Nucleolar organizer regions (NORs) are DNA coils that transcribe to ribosomal RNA. The NORassociated protein, termed argyrophilic NOR (AgNOR), was visible within the nucleus by staining with silver nitrate examination via the light microscope. AgNOR counting is a proliferation marker and may help in the diagnosis and prognosis of various neoplastic lesions. Aneuploidy (abnormal DNA content) can predict the progression, survival and prognosis of the tumors. The aim of this study was to evaluate the role of AgNORs, DNA ploidy status, and total S-phase fraction (TSPF) as prognostic parameters in malignant salivary gland tumors (MSGTs). Methods: The current study is a retrospective study on a cohort of MSGTs (N=47), to assess AgNORs using Silver Nitrate stain, DNA index (DI), and TSPF using flow cytometry (FCM). Data including tumor size and site, lymphovascular invasion (LVI), lymph node metastasis (LNM) were collected. Results: The AgNORs count was statistically significant with MSGT type. DI was found to have a significant association with tumor site, tumor size and MSGT type. In addition, TSPF was found to be significantly associated with LVI. A moderate positive correlation was noted between AgNORs count and TSPF. LNM, tumor site, high AgNORs and low DI were all associated with short disease-free survival (DFS) and poor overall survival (OS). Conclusion: The present study revealed that high AgNORs count, DNA aneuploidy and TSPF had a poor influence on MSGTs prognosis.

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Section: Discussionsupporting

confidence: 85%

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Hussien

Mohamed

Mansor

et al. 2022

Asian Pac J Cancer Prev

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“…[ 3 ] Regarding the histopathology, MECs are classified as low, intermediate, and high grade according to the relative number of epidermoid, mucous, and intermediate cell types, growth pattern, type of invasion, and cytological atypia. [ 2 4 5 6 ]…”

Section: Introductionmentioning

confidence: 99%

Expression of vascular endothelial-cadherin in mucoepidermoid carcinoma: Role in cancer development

Irani

Dehghan

2017

J Int Soc Prevent Communit Dent

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Objectives:Mucoepidermoid carcinoma (MEC) accounts for 35% of all malignant salivary gland tumors. Previous investigations have shown that vasculogenic mimicry (VM) exists in many cancers which can be used as a prognostic factor of poor prognosis. Elevated expression level of vascular endothelial (VE)-cadherin has been implicated in cancer neovascularization, growth, and progression. The current study aimed to study the presence of VE-cadherin in VM channels and tumor cells in different grades of MEC.Materials and Methods:A total of 63 MEC samples (21 samples in each grade) were collected from the archive of pathology department of Besat Educational Hospital, Hamadan, Iran, from 2002 to 2016. Hematoxylin and eosin staining was performed to confirm the previous diagnosis. The specimens were then processed for immunohistochemistry analysis. Then, periodic acid–Schiff staining was performed. Analyses were conducted through SPSS software version 22.0 (SPSS, Inc., Chicago, IL, USA). Chi-square test was used to examine the differences between categorical variables. Significance level was set at 0.05. Pearson's correlation was used to assess the co-localization of the marker.Results:A total of 63 samples (35 men; 55.6%, and 28 women; 44.4%) were used for immunohistochemical study. There were statistically significant differences between tumor grade and the expression levels of VE-cadherin (P = 0.000), between tumor grade and VM formation (P = 0.000), and also between tumor grade and microvessel density (MVD) (P = 0.000). Additionally, there was a strong positive correlation between tumor grade and VE-cadherin expression level (Pearson's r = 0.875, P < 0.000).Conclusions:Our results may disclose a definite relationship between VE-cadherin expression level, VM, epithelial–mesenchymal transition, cancer stem cells, and MVD in MEC samples. Thus, it is reasonable to suggest that VE-cadherin is related to angiogenesis and VM formation in MECs.

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“…Pleomorphic adenoma (PA) is the most common benign salivary gland tumor, constituting 45% to 85% of all major and minor salivary gland tumours. [ 1 2 ] It has been suggested that PA arises from myoepithelial cell and ductal cell differentiation into mesenchymal and epithelial structures. It is also believed that PA originates from reserve cells or stem cells of intercalated ducts which differentiate to mesenchymal and epithelial cells which may explain morphological diversity in PA, but the initiation factors of tumor progression are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

BRCA1/2 mutations in salivary pleomorphic adenoma and carcinoma-ex-pleomorphic adenoma

Irani¹,

Bidari-Zerehpoush²

2017

J Int Soc Prevent Communit Dent

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Objectives:It is hypothesized that carcinoma-ex-pleomorphic adenoma (Ca-ex-PA) is malignant from the beginning or is a malignant transformation of a recurrent or a long-standing pleomorphic adenoma (PA). The accumulation of genetic instabilities is suggested to be the main reason for malignant transformation in PA. The aim of this study was to determine the prevalence of BRCA1/2 mutations in PA and Ca-ex-PA.Materials and Methods:A total of ninety salivary gland tumors (45 Ca ex-PA and 45 PA) were selected. Immunohistochemistry was performed for all samples. Analyses were conducted through SPSS software version 22.0 (SPSS, Inc., Chicago, IL, USA). Chi-square test was used to examine the differences between the variables. Significant level was set at 0.05.Results:In general, 93.3% of PA samples showed positive staining for BRCA1 (in myoepithelial cells); however, BRCA2 positivity was found in 60% of samples (in myoepithelial cells). Among 45 samples of Ca-ex-PA, 93.3% of showed positivity for BRCA1 and 80% of samples showed positivity for BRCA2. Chi-square test found differences between PAs and Ca-ex-PAs regarding BRCA1/2 mutations in ductal cells and myoepithelial cells (P = 0.007, 0.000), respectively.Conclusions:The present study found a trend toward the presence of BRCA1/2 mutations in PA and Ca-ex-PA samples. Patients with BRCA1/2 mutation carriers are excellent cases for therapies, such as the poly (ADP) ribose polymerase inhibitor.

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How Useful is the AgNOR Staining Method for the Diagnosis of Salivary Gland Tumors?

Cited by 3 publications

References 20 publications

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Expression of vascular endothelial-cadherin in mucoepidermoid carcinoma: Role in cancer development

BRCA1/2 mutations in salivary pleomorphic adenoma and carcinoma-ex-pleomorphic adenoma

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