How to obtain a well-spread monolayer of lysozyme at the air/water interfaces

Interactions in Monolayers: A Study of the Behavior of Poly(methyl methacrylate)–Lysozyme Mixed Films from Surface Pressure–Area and Ellipsometric Measurements

“…Other factors that have an influence on the spreading of the protein, such as the elapsed time between the deposit of the film and the beginning of compression and the number of molecules deposited (i.e., the surface concentration of the protein, c s ) have been analyzed in a previous work, 21 leading to the conclusion that the best extension conditions are achieved when the number of molecules deposited is 1 Â 10 15 (c s = 2.1 m 2 /mg) and the waiting time is 3 h.…”

Interaction of surfactant and protein at the O/W interface and its effect on colloidal and biological properties of polymeric nanocarriers

“…Lysozyme is easily soluble in water but presents a rigid protein structure owed to strong intramolecular bonds (four disulfide bonds) [30,31]. Owing to this rigidity, most authors use denaturating agents to study lysozyme at interfaces [32,33].…”

Langmuir monolayer of lysozyme at variable subphase pH conditions: a comprehensive study on structure, morphology and hysteresis behaviour

“…As its pI is approximately 11.0, it behaves as a positively charged molecule under physiological conditions. 16 The primary structure of lysozyme comprises 129 amino acid residues with 14.3 kDa molecular mass. 17 The presence of four disulfide bonds makes the 3D structure of this protein unusually compact and highly stable.…”