Interactions in Monolayers: A Study of the Behavior of Poly(methyl methacrylate)–Lysozyme Mixed Films from Surface Pressure–Area and Ellipsometric Measurements

Conde, José Miñones; Conde, O.; Trillo, José Miñones; Miñones, J.

doi:10.1021/jp109111k

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…Compared to BSA, HEWL is a more "active" protein, as it exhibits antimicrobial activity as a lytic enzyme [39]. It is a protein with a molecular weight of 14.3 kDa and an isoelectric point of 11.35, making it cationic (total charge + 7) at neutral pH [40].…”

Section: Interaction Of Hen Egg-white Lysozyme (Hewl) With Hyaluronic...mentioning

confidence: 99%

Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR

et al. 2023

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According to actual literature data, hyaluronic acid (HA) that is presented in the extracellular matrix can interact with proteins and thereby affect several important functions of the cell membrane. The purpose of this work was to reveal the features of the interaction of HA with proteins using the PFG NMR method by sampling two systems: aqueous solutions of HA with bovine serum albumin (BSA) and aqueous solutions of HA with hen egg-white lysozyme (HEWL). It was found that the presence of BSA in the HA aqueous solution initiates a certain additional mechanism; as a result, the population of HA molecules in the gel structure increases to almost 100%. At the same time, for an aqueous solution of HA/HEWL, even in the range of low (0.01–0.2%) HEWL contents, strong signs of degradation (depolymerization) of some HA macromolecules were observed such that they lost the ability to form a gel. Moreover, lysozyme molecules form a strong complex with degraded HA molecules and lose their enzymatic function. Thus, the presence of HA molecules in the intercellular matrix, as well as in the state associated with the surface of the cell membrane, can, in addition to the known ones, perform one more important function: the function of protecting the cell membrane from the destructive action of lysozymes. The obtained results are important for understanding the mechanism and features of the interaction of extracellular matrix glycosaminoglycan with cell membrane proteins.

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Section: Interaction Of Hen Egg-white Lysozyme (Hewl) With Hyaluronic...mentioning

confidence: 99%

Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR

et al. 2023

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Molecular organization of bacterial membrane lipids in mixed systems—A comprehensive monolayer study combined with Grazing Incidence X-ray Diffraction and Brewster Angle Microscopy experiments

Wydro

Flasiński

Broniatowski

2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

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To properly design and investigate new antibacterial drugs a detailed description of the organization of bacterial membrane is highly important. Therefore in this work we performed a comprehensive characteristic of the Langmuir monolayers composed of phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) mixed in a wide range of composition and treated as an artificial cytoplasmic layer of bacterial membrane. To obtain detailed information on the properties of these films we combined the analysis of the surface pressure-area curves with the surface potential measurements, Brewster Angle Microscopy studies and Grazing Incidence X-ray Diffraction experiments. It was found that the investigated phospholipids mix nonideally in the monolayers and that the most favorable packing of molecules occurs at their equimolar proportion. This is directly connected with the formation of hydrogen bonds between both types of molecules in the system. All the collected experimental data evidenced that dipalmitoylphosphatidylethanolamine (DPPE) and dipalmitoylphosphatidylglycerol (DPPG) form highly ordered associates of fixed (DPPE:DPPG 1:1) stoichiometry. The obtained results allow one to conclude a nonuniform distribution of lipids in bacterial membranes and the existence of domains composed of the investigated phospholipids. The latter seems to be of great importance in the perspective of further studies on the mechanism of action of antibacterial agents.

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Monolayer and Brewster angle microscopy study of human serum albumin—Dipalmitoyl phosphatidyl choline mixtures at the air–water interface

Toimil

Prieto

Miñones

et al. 2012

Colloids and Surfaces B: Biointerfaces

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Interactions in Monolayers: A Study of the Behavior of Poly(methyl methacrylate)–Lysozyme Mixed Films from Surface Pressure–Area and Ellipsometric Measurements

Cited by 5 publications

References 40 publications

Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR

Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR

Molecular organization of bacterial membrane lipids in mixed systems—A comprehensive monolayer study combined with Grazing Incidence X-ray Diffraction and Brewster Angle Microscopy experiments

Monolayer and Brewster angle microscopy study of human serum albumin—Dipalmitoyl phosphatidyl choline mixtures at the air–water interface

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