How to Measure Cellular Shear Modulus Inside a Chip: Detailed Correspondence to the Fluid-Structure Coupling Analysis

Ito, Hiroaki; Takeishi, Naoki; Kirimoto, Atsushi; Chimura, Misato; Ohtani, Tomohito; Sakata, Yasushi; Horade, Mitsuhiro; Takayama, Toshio; Wada, Shigeo; Kaneko, Makoto

doi:10.1109/memsys.2019.8870772

Cited by 3 publications

(12 citation statements)

References 9 publications

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“…Kirimoto and the coauthors experimentally observed the flow profile surrounding a flowing RBC, and estimated the viscous stress on the surface of a RBC. Using the onchip manipulation, they measured step response [61] and dynamic responses of the amplitude and phase to a sinusoidal input [62,63], which provided the effective Young's modulus and viscosity consistent with the other conventional measurements [7,22].…”

Section: Active Control In Microfluidicsmentioning

confidence: 63%

“…In other words, the physical unit of force "N" lacked in the deformability measurement with the on-chip manipulation system. To overcome the limitation and obtain the full information of elastic and viscous coefficients, many efforts have been recently made [60][61][62][63][64]. The basic strategies can be classified into two concepts: (1) integration of the fluctuation analysis or (2) quantification of hydrodynamic force.…”

Section: Active Control In Microfluidicsmentioning

confidence: 99%

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On-chip cell manipulation and applications to deformability measurements

Ito

Kaneko

2020

Robomech J

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Active microfluidics for the applications to cellular deformability measurements is an emerging research field ranging from engineering to medicine. Here, we review conventional and microfluidic methods, and introduce an on-chip cell manipulation system with the design principle of fast and fine cell manipulation inside a microchannel. In the latter part of the review, we focus on the results of red blood cell (RBC) deformability measurements as one of the most frequently studied non-adherent cells by on-chip methods. The relationship between mechanical properties and biological structures/features, as well as medical/diagnostic applications, are also discussed.

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Section: Active Control In Microfluidicsmentioning

confidence: 63%

Section: Active Control In Microfluidicsmentioning

confidence: 99%

On-chip cell manipulation and applications to deformability measurements

Ito

Kaneko

2020

Robomech J

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“…Despite the studies referred to above, much is still unknown about the behavior of flowing RBCs, especially in a confined microchannel or between two closely spaced parallel plates (i.e., Hele-Shaw cell). Since the deformation of a RBC in a narrow rectangular microchannel is limited to an almost two-dimensional space, it is relatively easy to quantify the deformed configuration [17,18]. Although a number of studies using microfluidic devices have reported cellular-scale dynamics [19,20,21,22,23,24] as well as numerical studies [20,25,26,27,28], the dynamics of a translating RBC in a narrow rectangular microchannel have not yet been fully investigated.…”

Section: Introductionmentioning

confidence: 99%

“…Although a number of studies using microfluidic devices have reported cellular-scale dynamics [19,20,21,22,23,24] as well as numerical studies [20,25,26,27,28], the dynamics of a translating RBC in a narrow rectangular microchannel have not yet been fully investigated. Recent our developed on-chip feedback manipulation system allowed us to investigate the two-dimensionally projected shape profile of RBCs, and showed RBC heterogeneity in a narrow rectangular microchannel [17,18]. However, a precise deformation especially in thickness direction of RBCs cannot be captured by means of the experimental observation.…”

Section: Introductionmentioning

confidence: 99%

“…Fedosov et al (2014) systematically investigated the behavior of a single RBC in cylindrical microchannels for a wide range of channel confinements (2a/D, being the radius of the RBC a and the channel diameter D ) using a three-dimensional dissipative particle dynamics model [30]. However, their microchannels (2a/D< 0.8) had relatively large cross-sectional area comparing to a narrow rectangular microchannel represented in [17,18,31]. Zhu et al (2016) numerically investigated the behavior of a droplet in a Hele-Shaw cell, and identified characteristic flow structures that were induced by the translating droplet [31].…”

Section: Introductionmentioning

confidence: 99%

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Deformation of a Red Blood Cell in a Narrow Rectangular Microchannel

et al. 2019

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The deformability of a red blood cell (RBC) is one of the most important biological parameters affecting blood flow, both in large arteries and in the microcirculation, and hence it can be used to quantify the cell state. Despite numerous studies on the mechanical properties of RBCs, including cell rigidity, much is still unknown about the relationship between deformability and the configuration of flowing cells, especially in a confined rectangular channel. Recent computer simulation techniques have successfully been used to investigate the detailed behavior of RBCs in a channel, but the dynamics of a translating RBC in a narrow rectangular microchannel have not yet been fully understood. In this study, we numerically investigated the behavior of RBCs flowing at different velocities in a narrow rectangular microchannel that mimicked a microfluidic device. The problem is characterized by the capillary number C a , which is the ratio between the fluid viscous force and the membrane elastic force. We found that confined RBCs in a narrow rectangular microchannel maintained a nearly unchanged biconcave shape at low C a , then assumed an asymmetrical slipper shape at moderate C a , and finally attained a symmetrical parachute shape at high C a . Once a RBC deformed into one of these shapes, it was maintained as the final stable configurations. Since the slipper shape was only found at moderate C a , measuring configurations of flowing cells will be helpful to quantify the cell state.

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Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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How to Measure Cellular Shear Modulus Inside a Chip: Detailed Correspondence to the Fluid-Structure Coupling Analysis

Cited by 3 publications

References 9 publications

On-chip cell manipulation and applications to deformability measurements

On-chip cell manipulation and applications to deformability measurements

Deformation of a Red Blood Cell in a Narrow Rectangular Microchannel

Advances in Microfluidics for Single Red Blood Cell Analysis

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