On-chip cell manipulation and applications to deformability measurements

Ito, Hiroaki; Kaneko, Makoto

doi:10.1186/s40648-020-0154-x

Cited by 12 publications

(6 citation statements)

References 91 publications

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“…The reduced device size and small sample volumes lead to greater efficiency of the device, portability of the instrument and quick read-out data, thereby allowing for fast sampling times and disposability. Advanced microfluidic techniques have been used for single-cell analysis, especially for the measurement of cellular shape, size and deformability [ 99 ]. They are studied and reviewed from the viewpoint of two methods based on the controlled flow—the passive microfluidics drives a constant flow within the microchannel and has been widely used for deformability tests.…”

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

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Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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“…The deformability-based separation of IRBCs from RBCs demonstrates the potential of label-free enrichment of parasite-infected RBCs from the blood sample in a simple microfluidic device. In addition to the application in cell separation, the MSF phenomenon provides a new tool for the analysis of physical properties of infectious cells . Continuous, high throughput cell deformability assessment is needed for large-scale cell analysis .…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the application in cell separation, the MSF phenomenon provides a new tool for the analysis of physical properties of infectious cells. 52 Continuous, high throughput cell deformability assessment is needed for large-scale cell analysis. 53 The rapid screening of cell deformability can be realized utilizing high viscoelasticity flow cytometry, in which cell mechanical property is evaluated by measuring cell MSF response change under different flow rates in a confined channel.…”

Section: Discussionmentioning

confidence: 99%

Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow

et al. 2022

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Viscoelastic flow has been widely used in microfluidic particle separation processes, in which particles get focused on the channel center in diluted viscoelastic flow. In this paper, the transition from single-stream focusing to multiple-streams focusing (MSF) in high viscoelastic flow is observed, which is applied for cell separation processes. Particle focusing stream bifurcation is caused by the balance between elastic force and viscoelastic secondary flow drag force. The influence of cell physical properties, such as cell dimension, shape, and deformability, on the formation of multiple-streams focusing is studied in detail. Particle separation is realized utilizing different separation criteria. The size-based separation of red (RBC) and white (WBC) blood cells is demonstrated in which cells get focused in different streams based on their dimension difference. Cells with different deformabilities get stretched in the viscoelastic flow, leading to the change of focusing streams, and this property is harnessed to separate red blood cells infected with the malaria parasite, Plasmodium falciparum. The achieved results promote our understanding of particle movement in the high viscoelastic flow and enable new particle manipulation and separation processes for sample treatment in biofluids.

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“…However, discrepancies in the atomic force microscopy could arise due to cantilever calibration errors of 10-15%, speed of indentation, type of probe used, the probed area, and probed timescale [2]. Similarly, each technique is developed under the trade-off relationship between information content and the throughput [96,102].…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Narrow-Gap Rheometry: A Novel Method for Measuring Cell Mechanics

Bashir¹,

Suhyang

Jung

et al. 2022

Cells

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The viscoelastic properties of a cell cytoskeleton contain abundant information about the state of a cell. Cells show a response to a specific environment or an administered drug through changes in their viscoelastic properties. Studies of single cells have shown that chemical agents that interact with the cytoskeleton can alter mechanical cell properties and suppress mitosis. This envisions using rheological measurements as a non-specific tool for drug development, the pharmacological screening of new drug agents, and to optimize dosage. Although there exists a number of sophisticated methods for studying mechanical properties of single cells, studying concentration dependencies is difficult and cumbersome with these methods: large cell-to-cell variations demand high repetition rates to obtain statistically significant data. Furthermore, method-induced changes in the cell mechanics cannot be excluded when working in a nonlinear viscoelastic range. To address these issues, we not only compared narrow-gap rheometry with commonly used single cell techniques, such as atomic force microscopy and microfluidic-based approaches, but we also compared existing cell monolayer studies used to estimate cell mechanical properties. This review provides insight for whether and how narrow-gap rheometer could be used as an efficient drug screening tool, which could further improve our current understanding of the mechanical issues present in the treatment of human diseases.

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On-chip cell manipulation and applications to deformability measurements

Cited by 12 publications

References 91 publications

A Review of Single-Cell Adhesion Force Kinetics and Applications

A Review of Single-Cell Adhesion Force Kinetics and Applications

Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow

Narrow-Gap Rheometry: A Novel Method for Measuring Cell Mechanics

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