How the Pacific Oyster Responds to Ocean Acidification: Development and Application of a Meta-Analysis Based Adverse Outcome Pathway

Ducker, James H.; Falkenberg, Laura J.

doi:10.3389/fmars.2020.597441

Cited by 12 publications

(7 citation statements)

References 83 publications

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“…We further show that this reduction in shell parameters was not related to metabolic depression or to changes in net calcification or global gene expression. In contrast, a recent metaanalysis on C. gigas suggests that OA induces a reduction in shell thickness that is related to an overall transcriptional change inducing metabolic depression and alteration of calcification rate (Ducker & Falkenberg, 2020). Here we revisit these assumptions and find in agreement with other prior studies that no metabolic disruptions occurred while shell strength and thickness decreased at pH 7.…”

Section: Accepted Articlesupporting

confidence: 86%

“…In contrast, a recent meta‐analysis on C . gigas suggests that OA induces a reduction in shell thickness that is related to an overall transcriptional change inducing metabolic depression and alteration of calcification rate (Ducker & Falkenberg, 2020). Here, we revisit these assumptions and find in agreement with other prior studies that no metabolic disruptions occurred while shell strength and thickness decreased at pH 7.3–7.7 (Timmins‐Schiffman et al, 2014; Wright et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Here we determine the reaction norm of juvenile Pacific oyster Crassostrea gigas , one of the most cultivated invertebrate species in the world (FAO, 2020), over a wide range of pHs for macro‐physiological traits, membrane lipids, and gene expression. Although the impacts of OA on this model species have been intensively studied using scenario approaches, there is currently no consensus on its robustness to low pH (Ducker & Falkenberg, 2020). The establishment of reaction norm with biochemical and molecular data is a novel approach that provides new insights on the sensitivity of marine calcifiers to OA.…”

Section: Introductionmentioning

confidence: 99%

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Revisiting tolerance to ocean acidification: Insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Lutier

Poi

Gazeau

et al. 2022

Global Change Biology

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Studies on the impact of ocean acidification on marine organisms involve exposing organisms to future acidification scenarios which has limited relevance for coastal calcifiers living in a mosaic of habitats. Identification of tipping points beyond which detrimental effects are observed is a widely generalizable proxy of acidification susceptibility at the populational level. This approach is limited to a handful of studies that focus on only a few macro-physiological traits, thus overlooking the whole organism response. Here we develop a framework to analyze the broad macro-physiological and molecular responses over a wide pH range in juvenile oyster. We identify low tipping points for physiological traits at pH 7.3-6.9 that coincide with a major reshuffling in membrane lipids and transcriptome. In contrast, a drop in pH affects shell parameters above tipping points, likely impacting animal fitness. These findings were made possible by the development of an innovative methodology to synthesize and identify the main patterns of variations in large -omic datasets, fitting them to pH and identifying molecular tipping-points. We propose the broad application of our framework to the assessment of effects of global change on other organisms.

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Section: Accepted Articlesupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Revisiting tolerance to ocean acidification: Insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Lutier

Poi

Gazeau

et al. 2022

Global Change Biology

View full text Add to dashboard Cite

show abstract

“…We further show that this reduction in shell parameters was not related to metabolic depression or to changes in net calcification or global gene expression. In contrast, a recent meta-analysis on C. gigas suggests that OA induces a reduction in shell thickness that is related to an overall transcriptional change inducing metabolic depression and alteration of calcification rate (Ducker & Falkenberg, 2020). Here we revisit these assumptions and find in agreement with other prior studies that no metabolic disruptions occurred while shell strength decreased at pH 7.3-7.7 (NBS scale) (Timmins-Schiffman et al, 2014;Wright et al, 2018).…”

Section: Discussionsupporting

confidence: 85%

“…Although the impacts of OA on this model species have been intensively studied using scenario approaches, there is currently no consensus on its robustness to low pH (Ducker & Falkenberg, 2020). The establishment of reaction norm with biochemical and molecular data is a novel approach that provide new insights on the sensitivity of marine calcifiers to OA.…”

Section: Introductionmentioning

confidence: 99%

Revisiting tolerance to ocean acidification: insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Lutier

Poi

Gazeau

et al. 2021

Preprint

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Studies on the impact of ocean acidification on marine organisms involve exposing organisms to future acidification scenarios as projected for open ocean, which has limited relevance for coastal calcifiers. Characterization of reaction norms across a range of pH and identification of tipping points beyond which detrimental effects are observed has been limited and focus on only a few macro-physiological traits. Here we filled this knowledge gap by developing a framework to analyze the broad macro-physiological and molecular responses over a wide pH range of juvenile Pacific oyster, a model species for which the tolerance threshold to acidification remains unknown. We identify low tipping points for physiological traits at pH 7.3-6.9 that coincide with a major reshuffling in membrane lipids and transcriptome. In contrast, shell parameters exhibit effects with pH drop well before tipping points, likely impacting animal fitness. These findings were made possible by the development of an innovative methodology to synthesize and identify the main patterns of variations in large -omic datasets, fit them to pH and identify molecular tipping-points. We propose the application of our framework broadly to the assessment of effects of global change on other organisms.

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Quantification and characterization of microplastics ingested by mangrove oysters across West Africa

Mahu,

Vanderpuye-Orgle,

Boateng

et al. 2024

Environ Sci Pollut Res

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Microplastic ingestion by marine organisms presents a challenge to both ecosystem functioning and human health. We characterized microplastic abundance, shape, size, and polymer types ingested by the West African mangrove oyster, Crassostrea tulipa (Lamarck, 1819) sampled from estuaries and lagoons from the Gambia, Sierra Leone, Ghana, Benin, and Nigeria using optical microscopy and Fourier transform infrared (FTIR) techniques. A total of 780 microplastics were isolated in the whole tissues of the 250 oysters (n = 50 oysters per country). The abundance and distribution of microplastics in the oysters followed the pattern: the Gambia > Ghana > Sierra Leone > Nigeria > Benin. The Tanbi wetlands in the Gambia recorded the highest average of 10.50 ± 6.69 per oyster while the Ouidah lagoon in Benin recorded the lowest average of 1.80 ± 1.90 per oyster. Overall, microplastic numbers varied significantly (p < 0.05) among the five countries. Microfibers, particularly those within 1001–5000 μm size, dominated the total microplastic count with a few fragments and films. No spherical microplastics were isolated in the oysters. In the Sierra Leone and Benin oysters, fragments and films were absent in the samples. Microplastic between the 1001 and 5000 μm size class dominated the counts, followed by 501–1000 μm, 101–500 μm, and 51–100 μm. Five polymer groups namely polyethylene, polyester, nylon, polypropylene, and polyamide were identified across the five countries, with polyethylene occurring in oysters from all five countries and polyester occurring in all but the oysters from Nigeria. This diversity of polymers suggests varied sources of microplastics ingested by the studied oysters. The absence of microspheres across the five supports findings from other studies that they are the least ingested and highly egested by the oysters. Graphical Abstract

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How the Pacific Oyster Responds to Ocean Acidification: Development and Application of a Meta-Analysis Based Adverse Outcome Pathway

Cited by 12 publications

References 83 publications

Revisiting tolerance to ocean acidification: Insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Revisiting tolerance to ocean acidification: Insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Revisiting tolerance to ocean acidification: insights from a new framework combining physiological and molecular tipping points of Pacific oyster

Quantification and characterization of microplastics ingested by mangrove oysters across West Africa

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