How Red Blood Cells Process Nitric Oxide

Gladwin, Mark T.

doi:10.1161/circulationaha.116.024752

Cited by 7 publications

(3 citation statements)

References 13 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The functions of hemoglobin (HB) and myoglobin (MB) in the cardiovascular system are relatively well understood and include O 2 transport and storage, and NO and nitrite (NO 2 - ) signaling 1, 2, 21, 26, 27 . Their expression is not restricted to erythrocytes (for HB) or cardiomyocytes and striated skeletal muscle cells (for MB) but also extends to endothelial and vascular smooth muscle (VSM) cells 1, 2 .…”

Section: Discussionmentioning

confidence: 99%

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling

Jourd’heuil

Reilly

et al. 2017

ATVB

View full text Add to dashboard Cite

Objective The role of hemoglobin and myoglobin in the cardiovascular system is well established, yet other globins in this context are poorly characterized. Here, we examined the expression and function of cytoglobin (CYGB) during vascular injury. Approach and Results We characterized CYGB content in intact vessels and primary vascular smooth muscle (VSM) cells and used two different vascular injury models to examine the functional significance of CYGB in vivo. We found that CYGB was strongly expressed in medial arterial VSM and human veins. In vitro and in vivo studies indicated that CYGB was lost upon VSM cell de-differentiation. In the rat balloon angioplasty model, site-targeted delivery of adenovirus encoding shRNA specific for CYGB prevented its re-expression and decreased neointima formation. Similarly, four weeks after complete ligation of the left common carotid, Cygb knockout mice displayed little to no evidence of neointimal hyperplasia in contrast to their wild-type littermates. Mechanistic studies in the rat indicated that this was primarily associated with increased medial cell loss, TUNEL staining, and caspase-3 activation, all indicative of prolonged apoptosis. In vitro, CYGB could be re-expressed upon VSM stimulation with cytokines and hypoxia and loss of CYGB sensitized human and rat aortic VSM cells to apoptosis. This was reversed upon antioxidant treatment or NOS2 inhibition. Conclusions These results indicate that CYGB is expressed in vessels primarily in differentiated medial vascular smooth muscle cells where it regulates neointima formation and inhibits apoptosis after injury.

show abstract

Section: Discussionmentioning

confidence: 99%

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling

Jourd’heuil

Reilly

et al. 2017

ATVB

View full text Add to dashboard Cite

show abstract

“…However, taking into consideration these results, we cannot exclude that NO is responsible for the decrease in platelet aggregation. It is possible that direct increase in NO release from blood cells (e.g., from nitroso-hemoglobin (Hb-NO)) [ 8 , 16 ] is responsible for modification of aggregation. However, in our study, we did not determine the level of this fraction of NO.…”

Section: Discussionmentioning

confidence: 99%

Low‐Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers

Rola

Doroszko

Szahidewicz-Krupska

et al. 2017

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Aim The goal of the study is to develop a model allowing to investigate precisely the effect of low-level laser therapy (LLLT) on platelet aggregation and to verify the hypothesis regarding the role of the nitric oxide (NO) bioavailability and platelet activation markers in modulating platelet aggregation. Methods A total of 41 healthy volunteers at the age of 21–45 years were investigated. At first, platelet aggregation in response to three agonists (TRAP, ADP, and collagen) was evaluated following previous exposure to different doses of laser radiation (λ = 662 nm) to assess the dose-response effect. Subsequently, plasma levels of platelet activation markers (PF4—platelet factor-4 and sP-selectin) as well as the substrate for nitric oxide synthase, L-arginine, and its competitive inhibitors (ADMA—asymmetric dimethylarginine and SDMA—symmetric dimethylarginine) were measured. Results All doses of laser irradiation significantly reduced the aggregation. However, the most pronounced effect was observed for 19.7 J/cm2. No significant differences in the levels of platelet activation markers nor in the nitric-oxide-metabolic-pathway compounds between analyzed groups were noted. Conclusions We have demonstrated in the established in vitro experimental model that the LLLT in a reproducible manner decreases the whole blood platelet aggregation regardless of the NO bioavailability or changes in the platelet activation markers.

show abstract

“…In addition, compared with normal human hemoglobin, HBOC has a lower affinity for oxygen, which improves tissue oxygenation 21 . However, previous studies have found that HBOCs have side effects, such as oxidative stress, vascular activity, coagulation dysfunction, and nephrotoxicity 22,23 (Table 2). To reduce the side effects of HBOCs, researchers have conducted a series of animal experiments to determine the suitable dosage and application time.…”

Section: New Oxygen-carrying Plasmamentioning

confidence: 99%

Characteristics of New Oxygen-Carrying Plasma and Its Application Prospects in the Treatment of Severe Acute Pancreatitis

et al. 2023

View full text Add to dashboard Cite

Oxygen-carrying plasma, a new type of colloid substitute, is composed of hydroxyethyl starch and acellular hemoglobin-based oxygen carriers. It can supplement colloidal osmotic pressure and rapidly improve the body's oxygen supply. The resuscitation effect of the new oxygen-carrying plasma in animal shock models is better than that of hydroxyethyl starch or hemoglobin-based oxygen carriers alone. It can reduce the histopathological damage and mortality associated with severe acute pancreatitis, and it is expected to become an interesting treatment method for severe acute pancreatitis. This article reviews the characteristics of the new oxygen-carrying plasma, its role in fluid resuscitation, and its application prospects in the treatment of severe acute pancreatitis.

show abstract

How Red Blood Cells Process Nitric Oxide

Cited by 7 publications

References 13 publications

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling

Low‐Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers

Characteristics of New Oxygen-Carrying Plasma and Its Application Prospects in the Treatment of Severe Acute Pancreatitis

Contact Info

Product

Resources

About