Low‐Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers

Abstract

Aim The goal of the study is to develop a model allowing to investigate precisely the effect of low-level laser therapy (LLLT) on platelet aggregation and to verify the hypothesis regarding the role of the nitric oxide (NO) bioavailability and platelet activation markers in modulating platelet aggregation. Methods A total of 41 healthy volunteers at the age of 21–45 years were investigated. At first, platelet aggregation in response to three agonists (TRAP, ADP, and collagen) was evaluated following previous exposure to different doses of laser radiation (λ = 662 nm) to assess the dose-response effect. Subsequently, plasma levels of platelet activation markers (PF4—platelet factor-4 and sP-selectin) as well as the substrate for nitric oxide synthase, L-arginine, and its competitive inhibitors (ADMA—asymmetric dimethylarginine and SDMA—symmetric dimethylarginine) were measured. Results All doses of laser irradiation significantly reduced the aggregation. However, the most pronounced effect was observed for 19.7 J/cm2. No significant differences in the levels of platelet activation markers nor in the nitric-oxide-metabolic-pathway compounds between analyzed groups were noted. Conclusions We have demonstrated in the established in vitro experimental model that the LLLT in a reproducible manner decreases the whole blood platelet aggregation regardless of the NO bioavailability or changes in the platelet activation markers.

Low-Level Light Therapy Protects Red Blood Cells Against Oxidative Stress and Hemolysis During Extracorporeal Circulation

“…The inhibitory effect was reversible up to 1 h after the termination of irradiation ( Brill et al, 2000 ). More recently, Rola et al (2017) confirmed decreased whole blood aggregation after LLLT exposure by the mechanisms independent of the NO metabolism and without significant effect on the release of platelet activation markers. It is important because platelet activation due to contact with biomaterial of ECC or alternating shear stress results in P-selectin expression, which binds to P-selectin glycoprotein ligand-1 in the plasma membranes of leukocytes.…”

Therapeutic potential of intranasal photobiomodulation therapy for neurological and neuropsychiatric disorders: a narrative review

“…In addition to the described changes in tissue oxygenation and modifications of the RBC structure, the systemic effect of nostril-based i-PBMT via irradiation of blood cells could be directly related to the mechanism of hemostasis modulation. On one side, reversible suppression of the sensitivity of platelets to different agonists, and reduced activity of enzymes in the arachidonic acid cascade in a dose-dependent manner of PBMT was reported (Brill et al, 2000;Rola et al, 2017;Drohomirecka et al, 2018) leading to the conclusion that the PBMT could prevent platelets from undergoing apoptosis and prolong their lifespan under pathological conditions (Yang et al, 2016;Zhang et al, 2016Zhang et al, , 2018. Evidence has shown that the mitochondrial respiratory rate and ATP levels are impaired in the blood platelets of patients with depression (Hroudová et al, 2013).…”

Uso de autólogos associados á fotobiomodulação em harmonização orofacial de paciente fumante: relato de caso

“…Sistemicamente o paciente submetido ao estresse físico, devido à punção para colheita do sangue e posteriormente na aplicação do plasma rico em fibrinas na face, pode apresentar alteração dos batimentos, frequência cardíaca e da pressão arterial. Neste contexto, podemos incluir os benefícios da irradiação transcutânea do sangue, estudada amplamente na prevenção das doenças cardiovasculares e em seus efeitos antioxidantes para aumento da expectativa de vida [11][12][13] .…”