How industrial bacterial cultures can be kept stable over time

Wassenaar, Trudy M.; Zimmermann, Kurt

doi:10.1111/lam.13309

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…This is already seen in the high‐level original developments such as those listed in Voigt (2020). These examples demonstrate that we are on the verge of an enormous number of applications based on SynBio in vivo metabolic engineering and material improvement of extant microbes, notwithstanding the variation associated with inevitable evolution of all living organisms (Wassenaar and Zimmermann, 2020).…”

Section: A Future For In Vivo Microbial Studiesmentioning

confidence: 99%

In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology

Danchin

2021

Microbial Biotechnology

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Living systems are studied using three complementary approaches: living cells, cell-free systems and computer-mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico studies. This design-build-test-learn iteration loop cycle between experiments and analyses combines together physiology, genetics, biochemistry and bioinformatics in a way that keeps going forward. Because computeraided approaches are not directly constrained by the material nature of the entities of interest, we illustrate here how this virtuous cycle allows researchers to explore chemistry which is foreign to that present in extant life, from whole chassis to novel metabolic cycles. Particular emphasis is placed on the importance of evolution.

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Section: A Future For In Vivo Microbial Studiesmentioning

confidence: 99%

In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology

Danchin

2021

Microbial Biotechnology

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“…Bacterial culture stability is an important issue for industrial bacterial cultures. Therefore, preservation methods of long-term stored stocks culture and production procedure of bacteria culture is very important to be addressed (Wassenaar & Zimmermann, 2020).…”

Section: Optimization Of Medium Concentration On Chitinase Productionmentioning

confidence: 99%

Optimization of chitinase production from bacillus cereus smg 1.1 using response surface methodology

Widodo

Puspita²,

Ustadi³

2022

teknosains

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Shrimp shell waste are potential to be processed further into value-added products, such as N-acetylglucosamine (GlcNAc). In the production of GlcNAc, biological approach is preferred and environmentally friendly to chemical treatment. Chitinase is an enzyme that plays a vital role in bioprocessing of shrimp shell waste into GlcNAc. Previously, Bacillus cereus SMG 1.1 was isolated from fermented shrimp paste (terasi) and showed the ability to produce chitinase. This study was designed to determine the optimum medium for the production of chitinase from B. cereus SMG 1.1 through the Response Surface Method (RSM) using a factorial design with 15 treatments. The optimization of the media was carried out by determining the factors that influence the production of chitinase through the Plackett-Burman design followed by optimization of the concentration of the media through the Box-Behnken design. The test was designed to assess the effect of the independent variables on chitinase activity. Placket Burman analysis shows that colloidal chitin, fructose, and MgSO4.5H2O were the significant components in the medium affecting the production of chitinase by B. cereus SMG 1.1. Box-Behnken analysis developed a linear model capable of predicting the response. The highest response value was achieved at a concentration of 0.75% fructose, 1.5% colloidal chitin, and 0.075% MgSO4.5H2O resulted in optimum chitinase activity of 0.0016 U/ml.

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Potential probiotic lactobacilli strains isolated from artisanal Mexican Cocido cheese: evidence-based biosafety and probiotic action-related traits on in vitro tests

et al. 2023

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How industrial bacterial cultures can be kept stable over time

Cited by 3 publications

References 29 publications

In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology

In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology

Optimization of chitinase production from bacillus cereus smg 1.1 using response surface methodology

Potential probiotic lactobacilli strains isolated from artisanal Mexican Cocido cheese: evidence-based biosafety and probiotic action-related traits on in vitro tests

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