How do Nanoparticle Size and Protein Charge Affect Gold Nanoparticle-Protein Interactions?

Fitzkee, Nicholas C.; Wang, Ailin; Woods, Karen E.; Perera, Randika

doi:10.1016/j.bpj.2015.11.2832

Cited by 2 publications

(1 citation statement)

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“…Several studies on the characterization of amyloid fibrils and globular proteins were performed using SERS (Deyoung et al, 1993; Flynn et al, 2018; Kurouski, Handen, et al, 2015; Ortiz et al, 2007; Pauling, 1953). The introduction of a reproducible SERS substrate (Figure 3b) and an understanding of the protein–nanoparticle interactions can lead to a robust and ultrasensitive tool for proteomic studies (Chiti & Dobson, 2009; Fitzkee et al, 2016; Ghosh & Panicker, 2021; Karthigeyan et al, 2014; Koh et al, 2009; Lynch & Dawson, 2008; Mahmoudi et al, 2011; Siddhanta et al, 2012; Tavakol et al, 2018; Yadav & Siddhanta, 2022; You et al, 2005).…”

Section: Pers‐based Techniques For Studying Protein Aggregatesmentioning

confidence: 99%

Raman spectroscopy and its plasmon‐enhanced counterparts: A toolbox to probe protein dynamics and aggregation

Dhillon

Sharma

Yadav

et al. 2023

WIREs Nanomed Nanobiotechnol

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Protein unfolding and aggregation are often correlated with numerous diseases such as Alzheimer's, Parkinson's, Huntington's, and other debilitating neurological disorders. Such adverse events consist of a plethora of competing mechanisms, particularly interactions that control the stability and cooperativity of the process. However, it remains challenging to probe the molecular mechanism of protein dynamics such as aggregation, and monitor them in real‐time under physiological conditions. Recently, Raman spectroscopy and its plasmon‐enhanced counterparts, such as surface‐enhanced Raman spectroscopy (SERS) and tip‐enhanced Raman spectroscopy (TERS), have emerged as sensitive analytical tools that have the potential to perform molecular studies of functional groups and are showing significant promise in probing events related to protein aggregation. We summarize the fundamental working principles of Raman, SERS, and TERS as nondestructive, easy‐to‐perform, and fast tools for probing protein dynamics and aggregation. Finally, we highlight the utility of these techniques for the analysis of vibrational spectra of aggregation of proteins from various sources such as tissues, pathogens, food, biopharmaceuticals, and lastly, biological fouling to retrieve precise chemical information, which can be potentially translated to practical applications and point‐of‐care (PoC) devices.This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Diagnostic Tools > Diagnostic Nanodevices Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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