Protein-gold nanoparticle interactions and their possible impact on biomedical applications

Liu, Jingying; Peng, Qiang

doi:10.1016/j.actbio.2017.03.055

Cited by 152 publications

(86 citation statements)

References 147 publications

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“…However, this effect also has some drawbacks; when NPs are dispersed in biological fluids, diverse types of biomolecules (mainly proteins) readily adsorb onto the NPs forming the so-called protein corona (PC), which may dramatically change their biological identity, thereby affecting the uptake process and potentially blocking their targeting capabilities. 43 Approaches to improve cell uptake of NPs Modulation of NP physicochemical properties: Although endocytic uptake is the norm for a broad range of NPs, various structural features should be considered, including size, shape and surface chemistry. 41 Although there is no consensus regarding an optimum size that maximizes the level of cellular uptake, it seems clear that e.g.…”

Section: Strain-promoted Thiolmentioning

confidence: 99%

Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size

2018

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The primary function of the cell membrane is to protect cells from their surroundings. This entails a strict regulation on controlling the exchange of matter between the cell and its environment. A key factor when considering potential biological applications of a particular chemical structure has to do with its ability to internalize into cells. Molecules that can readily cross cell membranes are frequently needed in biological research and medicine, since most therapeutic entities are designed to modulate intracellular components. However, the design of molecules that do not penetrate cells is also relevant toward, for example, extracellular contrast agents, which are most widely used in clinical diagnosis. Small molecules have occupied the forefront of biomedical research until recently, but the past few decades have seen an increasing use of larger chemical structures, such as proteins or nanoparticles, leading to unprecedented and often unexpectedly novel research. Great achievements have been made toward understanding the rules that govern cellular uptake, which show that cell internalization of molecules is largely affected by their size. For example, macromolecules such as proteins and nucleic acids are usually unable to internalize cells. Intriguingly, in the case of nanoparticles, larger sizes seem to facilitate internalization via endocytic pathways, through which the particles remain trapped in lysosomes and endosomes. In this Account, we aimed at presenting our personal view of how different chemical structures behave in terms of cell internalization due to their size, ranging from small drugs to large nanoparticles. We first introduce the properties of cell membranes and the main mechanisms involved in cellular uptake. We then discuss the cellular internalization of molecules, distinguishing between those with molecular weights below 1 kDa and biological macromolecules such as proteins and nucleic acids. In the last section, we review the biological behavior of nanoparticles, with a special emphasis on plasmonic nanoparticles, which feature a high potential in the biomedical field. For each group of chemical structures, we discuss the parameters affecting their cellular internalization but also strategies that can be applied to achieve the desired intracellular delivery. Particular attention is paid to approaches that allow conditional regulation of the cell internalization process using external triggers, such as activable cell penetrating peptides, due to the impact that these systems may have in drug delivery and sensing applications. The Account ends with a "Conclusions and Outlook" section, where general lessons and future directions toward further advancements are briefly presented.

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Section: Strain-promoted Thiolmentioning

confidence: 99%

Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size

2018

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“…As an example, citrate‐stabilized AuNPs have been reported to be colloidally stabilized in the presence of proteins, but at the same time, AuNPs were reported to undergo aggregation in protein‐containing cultivation medium . Protein adsorption has been prominently reported for AuNPs . In many cases, the incubation of citrate‐stabilized AuNPs, e.g., with fetal bovine serum (FBS) containing medium, results in the increase of particle size as well as in the occurrence of some particle aggregates, which can be proven by different physicochemical methods .…”

Section: Particle Properties and Resulting Implications On Particle–cmentioning

confidence: 99%

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

2018

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between individual uptake mechanisms for respective nanoparticle systems. Cells internalizing nanoparticles respond to the nanoparticles and different intracellular processes will be initiated, possibly resulting in the impairment of the cellular key functions. [4] Even though these investigations provide data with statistical relevance, they can merely provide an overall picture of the uptake mechanism, as issues of different uptake mechanisms in anisotropic nanoparticle suspensions are barely assessable. Simultaneously, the influence of the biological environment, e.g., the presence of serum proteins, salts, or altered pH conditions, may have a severe impact on the particle properties, which can entail various biological implications. As such, the role of high resolution imaging techniques, which provide a clear evidence for the nanoparticles' fate in the cellular environment, [5] their localization and targeting to different cellular organelles, as well as evidence for uptake and release mechanisms, have gained increasing importance. In particular, transmission electron microscopy (TEM) investigations provide attractive possibilities to study the interaction of particles with biological barriers, i.e., the extracellular membrane or the nuclear membrane, their accumulation into cellular organelles or their spatial distribution on the scale of individual nanoparticles [6] (e.g., uptake as individual nanoparticles or in form of large clusters, etc.). In addition TEM can simultaneously image also the cellular membranes and organelles to determine respective morphological changes [7] upon nanoparticle interaction. [8][9][10][11] Thus, attractive possibilities to acquire additional information on the particle fate inside the cell emerge from TEM investigations.It can be stated here, that the utilization of electron microscopy has been conducted during the last decades [5] and, in view of some extent electron microscopy investigations of particle uptake, can be regarded in this sense as a routinely applied technique. However, frequently TEM is only utilized to characterize the nanoparticle system or to prove particle internalization. Only a fraction of reported studies utilize TEM analysis to reveal details of nanoparticle uptake and internalization or even deduce uptake pathways from these studies. This might be related to the fact that sometimes the interpretation of TEM data is not straightforward but a number of experimental limitations have to be taken into account for such studies. In a recent review, the advantages and disadvantages of TEM investigations in the context of the The internalization of nanoparticles into mammalian cells relies on a complex interplay of several parameters, which enable and dictate uptake and fate of nanoparticles in the cellular system. Due to the complexity of the involved processes, a careful experimental design has to be developed to elucidate peculiarities of the uptake processes of new nanoparticle systems into cells. Individual parameters can be hardly considered alone but h...

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“…First, due to physicochemical properties such as a large specific surface area and high adsorption capability, suspended nanoparticles can easily interact with proteins in saliva to form a nanoparticle–protein complex in the oral environment, and thereby may affect the formation of the acquired pellicle . The formation of this protein corona affects the structure and biological function of the protein molecule, and also changes the biological behavior of the nanoparticle.…”

Section: Antibiofilm Mechanisms Of Nanoparticles At Different Stages mentioning

confidence: 99%

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

Wang

Yuan

et al. 2019

Adv Healthcare Materials

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Due to their excellent size, designability, and outstanding targeted antibacterial effects, nanoparticles have become a potential option for controlling oral biofilm‐related infections. However, the formation of an oral biofilm is a dynamic process, and factors affecting the performance of antibiofilm treatments are complex. As such, when examining the existing literature on the antibiofilm effects of nanoparticles, attention should be paid to the specific mechanisms of action at different stages of oral biofilm formation, as well as relevant influencing factors, in order to achieve an objective and comprehensive evaluation. This review is intended to detail the antibacterial mechanisms of nanoparticles during the four stages of the formation of oral biofilms: 1) acquired film formation; 2) bacterial adhesion; 3) early biofilm development; and 4) biofilm maturation. In addition, factors influencing the antibiofilm properties of nanoparticles are summarized from the aspects of nanoparticles themselves, biofilm models, and host factors. The limitations of current research and possible trends for future research are also discussed. In summary, nanoparticles are a promising antioral biofilm strategy. It is hoped that this review can serve as a reference and inspire ideas for further research on the application of nanoparticles for effectively targeting and treating oral biofilms.

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Protein-gold nanoparticle interactions and their possible impact on biomedical applications

Cited by 152 publications

References 147 publications

Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size

Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

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