House dust mites and allergy.

Frankland, A. W.

doi:10.1136/adc.47.253.327

Cited by 11 publications

(3 citation statements)

References 15 publications

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“…Dermatophagoides farinae is one of the major components house dust (14)(15)(16)(17). In particular, it has been reported that the protein contained their deposits and shells causes allergy like bronchial asthma and allergic rhinitis (18,19). Therefore, rapid and sensitive methods are required to detect these allergenic substances.…”

Section: Resultsmentioning

confidence: 99%

A Fiber-Optic Fluorometric Immunoassay for Determination of Dermatophagoides Farinae Allergen (Der f1)

et al. 2008

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Immunoassay methods are generally used for measuring of allergenic substances. However, they need special facilities, cumbersome manipulation and taking a lot of time to get results. In this work, rapid and easy immunoassay using optical fiber system was established, which detected fluorescence of an immune complex formed by allergen and fluorescently labeled antibody. The immune complex was bound at the optical fiber probe surface, and excitation light was transmitted into the probe. Then evanescent wave was generated and excited fluorescent molecules, finally, fluorescent light was detected by a photo diode. Dermatophagoides farinae allergen (Der f1) was measured and cyanine 5 (Cy5) was used as fluorescent molecule. The fiber-optic immunoassay could detect and quantify Der f1. The measurement range was from 1.95ng/ml to 250 ng/ml, and the result was the equivalent of ELISA. The measurement time was 15min/sample.

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Section: Resultsmentioning

confidence: 99%

A Fiber-Optic Fluorometric Immunoassay for Determination of Dermatophagoides Farinae Allergen (Der f1)

et al. 2008

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“…No commercial extracts were used. We made up all allergenic extracts whether pollens, foods, animals or moulds, but when mites rather than moulds came to be recognised as an important allergen in the dust of damp houses (Frankland, 1972(Frankland, , 1991Frankland and E1 Hefny, 1971;Frankland et al, 1970), these were obtained commercially. Throughout the year much time was spent in the laboratory making up extracts other than pollen to test and treat patients.…”

Section: The Pollen Farmmentioning

confidence: 99%

Aerobiology and allergy — an autobiography

Frankland¹

1996

Aerobiologia

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“…Der f1 is derived from Dermatophagoides farinae, so-called house dust mite, and it is one of the most important indoor allergen (Chapman et al 1987a, b;Platts-Mills and Chapman 1987;Platts-Mills et al 1992;Platts-Mills and Weck 1989;Voorhost et al 1964;Stephenson 1991;Frankland 1972;Cunnington 1971;Takeda et al 1993). The immunoassay method for Der f1 using the fiber-optic fluorescence immunoassay system was established and enabled to measure Der f1 with rapidity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1)

Miyajima

Itabashi

Koshida

et al. 2011

Environ Monit Assess

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Immunoassay methods are generally used for measuring of allergenic substances. However, they need special facilities, skilled handling, and time-consuming procedure. In this work, a fiber-optic immunoassay system which could measure allergen by fluorescent intensities of immune complexes formed by allergens and fluorescently labeled antibodies was established. Immune complexes absorbed on the optical fiber probe surface, and excitation light was injected into the probe, then evanescent field is created in the proximity of the probe. The fluorophores were excited by the evanescent light, and fluorescence was detected by a photo diode. The target allergen detected by our system was Der f1 derived from Dermatophagoides farinae that is one of the house dust mite and major source of inhaled allergens. The fluorophore used labeling on detecting antibody was cyanine 5. The system enabled to detect and quantitatively determine of Der f1. The measurement range was from 0.24 to 250 ng/ml, and the result competes with ELISA. The measurement time was 16 min/sample. The immunoassay system was applied to measurement of Der f1 from actual dust samples. Calculated values of Der f1 showed good correlations between the fiber-optic fluoroimmunoassay and ELISA.

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House dust mites and allergy.

Cited by 11 publications

References 15 publications

A Fiber-Optic Fluorometric Immunoassay for Determination of Dermatophagoides Farinae Allergen (Der f1)

A Fiber-Optic Fluorometric Immunoassay for Determination of Dermatophagoides Farinae Allergen (Der f1)

Aerobiology and allergy — an autobiography

Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1)

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