House dust mite sensitivity in atopic children with Dectin-1 rs7309123 polymorphism

Abstract

Introduction: Sensitization to house dust mites is common in patients with atopic dermatitis. Dectin-1 polymorphism can affect the recognition of house dust mite allergens in the skin. Aim: To investigate the prevalence of sensitization to house dust mites in children with atopic dermatitis and the role of Dectin-1 rs7309123 polymorphism in the development of sensitivity to house dust mites. Material and methods:The study included 101 patients with atopic dermatitis. Genotyping of the Dectin-1 rs7309123 was performed in the patient group using real-time PCR. Measurements of sIgE to dust mites were performed by Western blotting. Results: Sensitization to house dust mites was found in 48.5% of the children. The presence of sIgE to HDM in patients with AD was significantly associated with concomitant allergic rhinitis (OR = 2.593 (1.096-6.133)) and in the group of children with both concomitant allergic diseases ). There was statistically significant difference in the distribution of genotypes in patients with elevated serum IgE to HDM and among the children with normal levels for CG and GG). Conclusions:The susceptibility to dust mites among children with atopic dermatitis is 48.5%. Dectin-1 is involved in the development of HDM-induced allergic inflammation in skin because polymorphism rs7309123 of the Dectin-1 gene may affect percutaneous sensitization to HDM in patients with AD, and the inhibition of signalling pathways of Dectin-1 may be an efficient therapeutic strategy.