House Dust Mite Induces Expression of Intercellular Adhesion Molecule-1 in EoL-1 Human Eosinophilic Leukemic Cells

Abstract

The house dust mite (HDM) is considered to be the most common indoor allergen associated with bronchial asthma. In this study, we investigated whether crude extract of the HDM Dermatophagoides farinae could activate human eosinophilic leukemic cells (EoL-1) to induce upregulation of cell-surface adhesion molecules. When EoL-1 cells were incubated with D. farinae extract, expression of intercellular adhesion molecule-1 (ICAM-1) significantly increased on the cell surfaces compared to cells incubated with medium alone. In contrast, surface expression of CD11b and CD49d in EoL-1 cells was not affected by D. farinae extract. In addition, pretreatment of cells with NF-κB inhibitor (MG-132) or JNK inhibitor (SP600125) significantly inhibited ICAM-1 expression promoted by HDM extract. However, neither p38 MAP kinase inhibitor nor MEK inhibitor prevented HDM-induced ICAM-1 expression in EoL-1 cells. These results suggest that crude extract of D. farinae induces ICAM-1 expression in EoL-1 cells through signaling pathways involving both NF-κB and JNK.

Effects of astragaloside IV on eosinophil activation induced by house dust mite allergen

“…Flow cytometric analysis confirmed previous findings that Der p 1 induces the expression of ICAM-1 and CCR3 by eosinophils (9,10). NF-κB has been suggested to be involved in the Der p 1-induced expression of ICAM-1 and CCR3 by eosinophils.…”

“…In vitro, the allergen activates NF-κB in human eosinophils, as shown by the upregulation of the expression of adhesion molecules (9,10) and the production of inflammatory cytokines (10,11), and selectively recruits airway dendritic cells upon the activation of the airway epithelium (12). Furthermore, the recurrent exposure of newborn monkeys to the house dust mite (HDM) aeroallergen has been found to result in the significant recruitment of eosinophils into the airway mucosa (13).…”