House dust allergy and immunotherapy

Thomas, Wayne R.

doi:10.4161/hv.20812

Cited by 34 publications

(35 citation statements)

References 153 publications

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“…3-A), aqueous HDM allergens are indeed major trigger of allergy among the Kapampangans, consistent with previous studies showing that 95% of the 210 Filipino allergic subjects are sensitized with allergens from any of the three species of HDMs [13]. The high incidence of elevated IgE reactivity greater than 100 ng/mL or 42 IU/mL indicates that HDM allergens are clinically potent indices of IgE production that can lead to severe nasal allergic reaction as implicated by earlier studies [14].…”

Section: Figsupporting

confidence: 74%

Allergenicity Profile of Fifteen Aqueous Allergen Extracts to Selected Allergic Individuals from Pampanga, Philippines

Totaan¹

2017

IJASTR

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Allergies are the most common disorders of the immune system, estimated to affect about 20% of the general population. In the local setting, there is a dearth of information on the sensitization profiles of allergic individuals from Pampanga, Philippines to different allergen sources, thus, evaluation of the total and allergen-specific IgE levels of 100 sera samples from allergic asthma, rhinitis and dermatitis" individuals and 100 sera from non-allergic subjects to fifteen aqueous protein extracts were performed. The aqueous protein extracts prepared from three house dust mite species, three species of cockroaches, two species of fungi, pollens from two species of plants, peanut and four seafoods were quantitated using the BioRad Protein Assay. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed multiple bands ranging from 10-250 kDa. Enzyme-linked immunosorbent assay (ELISA) revealed significantly higher mean total serum IgE levels among the allergic groups than non-allergic subjects (p<0.0001). Unpaired t-test results indicated that except for the fungal, tree pollen and crab allergens, there is an extremely significant difference between the mean allergen-specific IgE of allergic subjects compared to the non-allergic subjects for each of the other eleven aqueous allergen extracts, taken separately. Of the allergic subjects, 72.00% exhibited multiple sensitizations to the fifteen aqueous allergen extracts. This study provides the allergenicity profile of "Kapampangan" allergic individuals which could pave the way for the design of highly specific and accurate diagnostic protocols for allergies among the local patients and may serve as an important basis for immunotherapy.

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Section: Figsupporting

confidence: 74%

Allergenicity Profile of Fifteen Aqueous Allergen Extracts to Selected Allergic Individuals from Pampanga, Philippines

Totaan¹

2017

IJASTR

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“…These potent allergens account for 50–60 % of anti-house dust mite IgE antibodies in allergic subjects [7]. The group 1 allergens, Der p 1 and Der f 1, are cysteine proteases.…”

Section: Introductionmentioning

confidence: 99%

Indoor Allergens and Allergic Respiratory Disease

Pomés

Chapman

Wünschmann

2016

Curr Allergy Asthma Rep

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Purpose of review The purpose of this review is to evaluate the most recent findings on indoor allergens and their impact on allergic diseases. Recent findings Indoor allergens are present inside buildings (home, work environment, school), and given the chronic nature of the exposures, indoor allergies tend to be associated with the development of asthma. The most common indoor allergens are derived from dust mites, cockroaches, mammals (including wild rodents and pets), and fungi. The advent of molecular biology and proteomics has led to the identification, cloning, and expression of new indoor allergens, which have facilitated research to elucidate their role in allergic diseases. This review is an update on new allergens and their molecular features, together with the most recent reports on their avoidance for allergy prevention and their use for diagnosis and treatment. Summary Research progress on indoor allergens will result in the development of new diagnostic tools and design of coherent strategies for immunotherapy.

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“…Several studies show the importance of group 5 allergens in respiratory diseases [9, 11-14]. Others show that Der p 5 is strongly related to the development of asthma in allergic patients [3, 15, 16] and that significantly higher concentrations of specific IgE (sIgE) to Der p 5 can be observed in asthmatic patients. Furthermore, Der p 5 is secreted in very small quantities, and the number of mite-allergic patients specifically recognizing Der p 5 is disproportionately high considering its low expression level [5].…”

Section: Introductionmentioning

confidence: 99%

Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from Dermatophagoides pteronyssinus and Blomia tropicalis

Lahiani¹,

Dumez²,

Khemili³

et al. 2018

Int Arch Allergy Immunol

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Background: The aim of this work was to understand the molecular features that trigger the cross-reactivity observed between Der p 5 from Dermatophagoides pteronyssinus, Blo t 5 from Blomia tropicalis, and Der f 5 from D. farinae. Methods: We collected serum from 60 house dust mite (HDM)-allergic patients residing in the Dellys area of Boumerdès province in northern Algeria. The presence of specific IgE to Der p 5, Der f 5, and Blo t 5 was analyzed. We performed in silico analysis of the structure of the different allergens in order to identify epitopes that can elicit the cross-reactivity of the sera. Synthetic peptides corresponding to the linear epitope sequence of Der p 5, Der f 5, and Blo t 5 were used to evaluate its implication in the cross-reactivity between the allergens. We also modified the sequence of the conformational epitope of Der p 5 by site-directed mutagenesis to mimic Blo t 5. Results: Several sera of patients allergic to HDM contained specific IgE antibodies to Der p 5 and Blo t 5. We demonstrated that the linear epitope of Der p 5 and Blo t 5 is not involved in the cross-reactivity of the sera. Furthermore, mutations introduced in the sequence of Der p 5 to mimic Blo t 5 could not modulate the cross-reactivity between them. Conclusions: The major linear IgE epitopes of Der p 5 and Blo t 5 are involved in species-specific recognition. Our results may be useful for the development of a hypoallergenic vaccine against HDM group 5 allergens.

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House dust allergy and immunotherapy

Cited by 34 publications

References 153 publications

Allergenicity Profile of Fifteen Aqueous Allergen Extracts to Selected Allergic Individuals from Pampanga, Philippines

Allergenicity Profile of Fifteen Aqueous Allergen Extracts to Selected Allergic Individuals from Pampanga, Philippines

Indoor Allergens and Allergic Respiratory Disease

Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from <b><i>Dermatophagoides pteronyssinus</i></b> and <b><i>Blomia tropicalis</i></b>

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