Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from &lt;b&gt;&lt;i&gt;Dermatophagoides pteronyssinus&lt;/i&gt;&lt;/b&gt; and &lt;b&gt;&lt;i&gt;Blomia tropicalis&lt;/i&gt;&lt;/b&gt;

Lahiani, Sadjia; Dumez, Marie-Eve; Khemili, Souad; Bitam, Idir; Gilis, Dimitri; Galleni, Moreno

doi:10.1159/000492871

Cited by 9 publications

(5 citation statements)

References 34 publications

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“…In addition, the fine mapping of epitopes of Der p 5 was performed, and the amino acids involved in IgE binding were also determined in our laboratory. Indeed, the amino acid sequence ( 90 D-M 108 ) exhibited strong IgE binding; aromatic and hydrophobic residues are recognized as the most critical [1], [27].…”

Section: Discussionmentioning

confidence: 99%

Der p 5 allergen from house dust mite: first epitope mapping of rabbit IgG blocking antibodies

Lahiani

Dumez

Bitam

et al. 2019

New Microbes and New Infections

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Der p 5 is one of the important house dust mite allergens in Algeria; this allergen is frequently recognized by patients with allergic asthma. However, there is no information on its IgG–binding epitopes. In the present study, rabbits were immunized with recombinant Der p 5 allergen, and serum samples were obtained. Recognition of linear IgG epitopes of Der p 5 was determined using synthesized peptides derived from the allergen sequence. The results showed that serum from immunized rabbits recognized three linear epitopes from Der p 5 ( 28 EDKKHDYQNEFDFLLMERIHEQIK 43 ), ( 37 IHEQIKKGELALFYLQEQ 55 ) and ( 92 LMQRKDLDIFEQYNLEMAKKS 112 ). More interestingly, we observed that the 92 L-S 112 amino acid sequence is well recognized by both IgE and IgG antibodies. Der p 5 stimulates the synthesis of specific IgG antibodies which recognize common but also novel epitopes compared to IgE antibody binding. Indeed, the potential to induce IgG antibodies can be used to inhibit human IgE binding to allergens which may be part of the mechanism of action of specific immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Der p 5 allergen from house dust mite: first epitope mapping of rabbit IgG blocking antibodies

Lahiani

Dumez

Bitam

et al. 2019

New Microbes and New Infections

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“…[18][19][20][21] The major linear IgE epitopes of Der p 5 and Blo t 5 are involved in species-specific recognition. 22 Traditionally, OVA, an egg allergen, has been used in animal asthma models (5pc6), but has been almost replaced by respiratory allergens, such as Dermatophagoides pteronyssinus [23][24][25] and Blomia tropicalis. [26][27][28] And a new mouse model of severe asthma caused by Blo t 5 has been developed, which results in extensive lung inflammation, a strong adoptive and host Th2 response, airway remodeling, and the formation of IL4/IL-13-dependent iBALT.…”

Section: Introductionmentioning

confidence: 99%

Studies on HBcAg-rBlo t 5-21 Fusion Protein Vaccine That Alleviates Blomia tropicalis Airway Inflammation

Pei¹,

Xiao²,

Sang³

et al. 2022

JIR

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Background In tropical and subtropical areas, allergens from the dust mite species Blomia tropicalis are common causes of allergic rhinitis and asthma. Blomia tropicalis has two main allergens: Blo t 5 and Blo t 21. Aim To generate a chimeric virus-like particle containing HBcAg, Blo t 5 and Blo t 21 that can treat allergies caused by Blomia tropicalis . Methods To produce allergic asthma in mice, prokaryotic expression and purification of Blomia tropicalis allergens rBlo t 5, rBlo t 21, and recombinant fusion allergen rBlo t 5–21 were utilized in the study. We created a hepatitis B core antigen (HBcAg) and rBlo t 5–21 fusion prokaryotic expression plasmid. HBcAg-rBlo t 5–21 was purified after expression and tested by transmission electron microscopy (TEM). Furthermore, the protein HBcAg-rBlo t 5–21 was employed as a protein vaccination. Results In allergy-induced mouse model experiments, the fusion allergen rBlo t 5–21 was more effective than the individual allergens rBlo t 5 and rBlo t 21 at inducing allergy. We found that vaccinating allergic mice with the recombinant fusion protein vaccine HBcAg-rBlo t 5–21 alleviated allergy symptoms elicited by the rBlo t 5–21 allergen. Vaccination with HBcAg-rBlo t 5–21 resulted in a decrease in total serum IgE levels, suppression of anaphylaxis, and reduction of inflammatory cell infiltration into lung tissue as compared to the PBS group. Conclusion HBcAg-rBlo t 5–21, a protein vaccine containing both the hepatitis B core antigen and the Blomia tropicalis fusion allergen rBlo t 5–21, could be a suitable vaccination for preventing allergy disorders caused by Blomia tropicalis .

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“…2,5,7,8 Several allergenic molecules have been identified in the extract of Blomia tropicalis and the most important of them is the group 5 allergen, Blo t 5 (14 KDa). [9][10][11] Contamination of food during storage by mites may cause significant loss of product quality, since these insects feed on the germ of the grains causing a decrease in the germination viability of seeds, besides losses in the nutritional composition. 12,13 Many elements with pathogenic properties, including allergenic contaminants, can be present in foods.…”

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confidence: 99%

A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

Alves

Notário

Correia

et al. 2023

J. Electrochem. Soc.

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The present study focuses on the first electrochemical immunosensor built with polymeric mats enriched with nanomaterials, targeting the dust mite protein, for the detection of allergens in flour sources with important implications in allergic reactions. As a proof-of-concept, we have used the Blo t 5 allergen from Blomia tropicalis (target) and established electrochemical parameters to recognize and detect the specific allergen using a polyclonal immunoglobulin (Ig) Y (probe). Detection was performed in a portable potentiostat (EmStat) using a graphite screen-printed electrode. For that, the nanofibrous mats of poly(lactic acid)/poly(ethylene glycol), and carbon nanotubes were placed in contact with the surface of the electrodes. The functionalization that occurs through the deposition of the polymer was induced through the passage of current. Subsequently, the modification was validated by cyclic voltammetry (CV) readings and ferri/ferrocyanide was used as a redox indicator in CV analyses. The novel immunosensor was able to discriminate between allergen-contaminated and uncontaminated farinaceous samples. The immunosensor displays high sensitivity detecting up to 50 µg/ml in the calibration curve with a linear response between 5 and 500 µg/ml. The new biosensor shows great potential in the detection of farinaceous contaminants in laboratories, food factories and in the field.

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Cross-Reactivity between Major IgE Epitopes of Family 5 Allergens from <b><i>Dermatophagoides pteronyssinus</i></b> and <b><i>Blomia tropicalis</i></b>

Cited by 9 publications

References 34 publications

Der p 5 allergen from house dust mite: first epitope mapping of rabbit IgG blocking antibodies

Der p 5 allergen from house dust mite: first epitope mapping of rabbit IgG blocking antibodies

Studies on HBcAg-rBlo t 5-21 Fusion Protein Vaccine That Alleviates Blomia tropicalis Airway Inflammation

A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

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