HOTAIR rs7958904 polymorphism is associated with increased cervical cancer risk in a Chinese population

Abstract

Previously, we have identified single nucleotide polymorphisms (SNPs) rs7958904 and rs4759314 in long non-coding RNA HOX transcript antisense RNA (HOTAIR) were significantly associated with risk of colorectal and gastric cancer, respectively. Here, we aimed to investigate the association between HOTAIR SNPs and cervical cancer (CC) susceptibility. A total of 1209 cases and 1348 controls were enrolled for association study and genotyped with TaqMan allelic discrimination method. The Cancer Genome Atlas (TCGA) database was utilized for in vivo analysis of allele-specific HOTAIR expression. MTT assay was employed for evaluation of allele-specific cell proliferation. The rs7958904 CC genotype was related to an increased risk of cervical cancer compared with the GG/GC genotypes (OR = 1.57, 95% CI = 1.10–2.25). TCGA database showed the CC tissues with rs7958904 CC genotype had higher HOTAIR expression than those with GG genotype (P = 0.046). MTT assay demonstrated a growth-promoting role of rs7958904 C allele on CC cells. Further functional studies on the effect of rs7958904 on biological behavior of CC cells are needed to confirm and extend our findings. In conclusion, HOTAIR rs7958904 might influence CC susceptibility through modulation of CC cell proliferation, and could serve as a diagnostic biomarker.

HOTAIR: An Oncogenic Long Non-Coding RNA in Human Cancer

“…The analysis using TCGA data indicated that CC tissues with the rs7958904 CC genotype exhibited higher HOTAIR expression compared with those carrying the GG genotype. Thus, HOTAIR rs7958904 could influence CC susceptibility, and HOTAIR could be used as a diagnostic biomarker [100]. Another report demonstrated that the genetic variant exhibited an alteration in secondary structure propensities and a gain of a miR-22 binding site in HOTAIR, which was found to be concordant with the overexpression of miR-22 in low HOTAIR cases, compared with that in the control.…”

HOTAIR gene polymorphisms contribute to increased neuroblastoma susceptibility in Chinese children

“…66 The rs4759314 A>G polymorphism, located in TFBS, was associated with gastric cancer and EOC, 39 and the rs7958904 G>C polymorphism, located in exon 6 of HOTAIR, 67 was associated with colorectal cancer, EOC, osteosarcoma, 39 and cervical cancer. 67 Along with the previous reports cited above, the results of the current study suggest that the genetic variation of HOTAIR is complex and depends on cancer types. The inconsistent, even opposite, effects on the risk of the same cancer type may reflect the complexity of HOTAIR function.…”

“…The rs920778 A>G polymorphism, located in the intronic enhancer of HOTAIR , had a genotype‐specific effect on HOTAIR expression, and was associated with breast cancer and cervical cancer . The rs4759314 A>G polymorphism, located in TFBS, was associated with gastric cancer and EOC, and the rs7958904 G>C polymorphism, located in exon 6 of HOTAIR , was associated with colorectal cancer, EOC, osteosarcoma, and cervical cancer . Along with the previous reports cited above, the results of the current study suggest that the genetic variation of HOTAIR is complex and depends on cancer types.…”

Clinical Value of Long Noncoding RNA HOTAIR as a Novel Biomarker in Digestive Cancers: A Meta-Analysis

“…Jin et al demonstrated that HOTAIR rs7958904 might influence cervical cancer susceptibility through modulation of cervical cancer cell proliferation and could serve as a diagnostic biomarker. 48 It has been reported that the predisposition for cervical cancer might be associated with one kind of single nucleotide polymorphism (SNP) in HOTAIR (rs920778). 49 Thus, it is important and necessary to combine these published data through meta-analysis and evaluate the association between expression of HOTAIR and prognosis as well as clinicopathological characteristics in patients with digestive cancer.…”